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A novel fluorescent pH probe for expression in plants

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A novel fluorescent pH probe for expression in plants. / Schulte, Alexander; Lorenzen, Inken; Böttcher, Markus; Plieth, Christoph.

In: PLANT METHODS, Vol. 2, 01.01.2006, p. 7.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Schulte, A, Lorenzen, I, Böttcher, M & Plieth, C 2006, 'A novel fluorescent pH probe for expression in plants', PLANT METHODS, vol. 2, pp. 7. https://doi.org/10.1186/1746-4811-2-7

APA

Schulte, A., Lorenzen, I., Böttcher, M., & Plieth, C. (2006). A novel fluorescent pH probe for expression in plants. PLANT METHODS, 2, 7. https://doi.org/10.1186/1746-4811-2-7

Vancouver

Schulte A, Lorenzen I, Böttcher M, Plieth C. A novel fluorescent pH probe for expression in plants. PLANT METHODS. 2006 Jan 1;2:7. https://doi.org/10.1186/1746-4811-2-7

Bibtex

@article{139655534b554bce9f8c8afe3096a10c,
title = "A novel fluorescent pH probe for expression in plants",
abstract = "BACKGROUND: The pH is an important parameter controlling many metabolic and signalling pathways in living cells. Recombinant fluorescent pH indicators (pHluorins) have come into vogue for monitoring cellular pH. They are derived from the most popular Aequorea victoria GFP (Av-GFP). Here, we present a novel fluorescent pH reporter protein from the orange seapen Ptilosarcus gurneyi (Pt-GFP) and compare its properties with pHluorins for expression and use in plants.RESULTS: pHluorins have a higher pH-sensitivity. However, Pt-GFP has a broader pH-responsiveness, an excellent dynamic ratio range and a better acid stability. We demonstrate how Pt-GFP expressing Arabidopsis thaliana report cytosolic pH-clamp and changes of cytosolic pH in the response to anoxia and salt-stress.CONCLUSION: Pt-GFP appears to be the better choice when used for in vivo-recording of cellular pH in plants.",
author = "Alexander Schulte and Inken Lorenzen and Markus B{\"o}ttcher and Christoph Plieth",
year = "2006",
month = jan,
day = "1",
doi = "10.1186/1746-4811-2-7",
language = "English",
volume = "2",
pages = "7",
journal = "PLANT METHODS",
issn = "1746-4811",
publisher = "BioMed Central Ltd.",

}

RIS

TY - JOUR

T1 - A novel fluorescent pH probe for expression in plants

AU - Schulte, Alexander

AU - Lorenzen, Inken

AU - Böttcher, Markus

AU - Plieth, Christoph

PY - 2006/1/1

Y1 - 2006/1/1

N2 - BACKGROUND: The pH is an important parameter controlling many metabolic and signalling pathways in living cells. Recombinant fluorescent pH indicators (pHluorins) have come into vogue for monitoring cellular pH. They are derived from the most popular Aequorea victoria GFP (Av-GFP). Here, we present a novel fluorescent pH reporter protein from the orange seapen Ptilosarcus gurneyi (Pt-GFP) and compare its properties with pHluorins for expression and use in plants.RESULTS: pHluorins have a higher pH-sensitivity. However, Pt-GFP has a broader pH-responsiveness, an excellent dynamic ratio range and a better acid stability. We demonstrate how Pt-GFP expressing Arabidopsis thaliana report cytosolic pH-clamp and changes of cytosolic pH in the response to anoxia and salt-stress.CONCLUSION: Pt-GFP appears to be the better choice when used for in vivo-recording of cellular pH in plants.

AB - BACKGROUND: The pH is an important parameter controlling many metabolic and signalling pathways in living cells. Recombinant fluorescent pH indicators (pHluorins) have come into vogue for monitoring cellular pH. They are derived from the most popular Aequorea victoria GFP (Av-GFP). Here, we present a novel fluorescent pH reporter protein from the orange seapen Ptilosarcus gurneyi (Pt-GFP) and compare its properties with pHluorins for expression and use in plants.RESULTS: pHluorins have a higher pH-sensitivity. However, Pt-GFP has a broader pH-responsiveness, an excellent dynamic ratio range and a better acid stability. We demonstrate how Pt-GFP expressing Arabidopsis thaliana report cytosolic pH-clamp and changes of cytosolic pH in the response to anoxia and salt-stress.CONCLUSION: Pt-GFP appears to be the better choice when used for in vivo-recording of cellular pH in plants.

U2 - 10.1186/1746-4811-2-7

DO - 10.1186/1746-4811-2-7

M3 - SCORING: Journal article

C2 - 16600023

VL - 2

SP - 7

JO - PLANT METHODS

JF - PLANT METHODS

SN - 1746-4811

ER -