A novel fluorescent pH probe for expression in plants
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A novel fluorescent pH probe for expression in plants. / Schulte, Alexander; Lorenzen, Inken; Böttcher, Markus; Plieth, Christoph.
in: PLANT METHODS, Jahrgang 2, 01.01.2006, S. 7.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - A novel fluorescent pH probe for expression in plants
AU - Schulte, Alexander
AU - Lorenzen, Inken
AU - Böttcher, Markus
AU - Plieth, Christoph
PY - 2006/1/1
Y1 - 2006/1/1
N2 - BACKGROUND: The pH is an important parameter controlling many metabolic and signalling pathways in living cells. Recombinant fluorescent pH indicators (pHluorins) have come into vogue for monitoring cellular pH. They are derived from the most popular Aequorea victoria GFP (Av-GFP). Here, we present a novel fluorescent pH reporter protein from the orange seapen Ptilosarcus gurneyi (Pt-GFP) and compare its properties with pHluorins for expression and use in plants.RESULTS: pHluorins have a higher pH-sensitivity. However, Pt-GFP has a broader pH-responsiveness, an excellent dynamic ratio range and a better acid stability. We demonstrate how Pt-GFP expressing Arabidopsis thaliana report cytosolic pH-clamp and changes of cytosolic pH in the response to anoxia and salt-stress.CONCLUSION: Pt-GFP appears to be the better choice when used for in vivo-recording of cellular pH in plants.
AB - BACKGROUND: The pH is an important parameter controlling many metabolic and signalling pathways in living cells. Recombinant fluorescent pH indicators (pHluorins) have come into vogue for monitoring cellular pH. They are derived from the most popular Aequorea victoria GFP (Av-GFP). Here, we present a novel fluorescent pH reporter protein from the orange seapen Ptilosarcus gurneyi (Pt-GFP) and compare its properties with pHluorins for expression and use in plants.RESULTS: pHluorins have a higher pH-sensitivity. However, Pt-GFP has a broader pH-responsiveness, an excellent dynamic ratio range and a better acid stability. We demonstrate how Pt-GFP expressing Arabidopsis thaliana report cytosolic pH-clamp and changes of cytosolic pH in the response to anoxia and salt-stress.CONCLUSION: Pt-GFP appears to be the better choice when used for in vivo-recording of cellular pH in plants.
U2 - 10.1186/1746-4811-2-7
DO - 10.1186/1746-4811-2-7
M3 - SCORING: Journal article
C2 - 16600023
VL - 2
SP - 7
JO - PLANT METHODS
JF - PLANT METHODS
SN - 1746-4811
ER -