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A new method to isolate large numbers of rabbit osteoclasts and osteoclast-like cells

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A new method to isolate large numbers of rabbit osteoclasts and osteoclast-like cells : application to the characterization of serum response element binding proteins during osteoclast differentiation. / David, J P; Neff, L; Chen, Y; Rincon, M; Horne, W C; Baron, R.

In: J BONE MINER RES, Vol. 13, No. 11, 01.11.1998, p. 1730-8.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

David, JP, Neff, L, Chen, Y, Rincon, M, Horne, WC & Baron, R 1998, 'A new method to isolate large numbers of rabbit osteoclasts and osteoclast-like cells: application to the characterization of serum response element binding proteins during osteoclast differentiation', J BONE MINER RES, vol. 13, no. 11, pp. 1730-8. https://doi.org/10.1359/jbmr.1998.13.11.1730

APA

David, J. P., Neff, L., Chen, Y., Rincon, M., Horne, W. C., & Baron, R. (1998). A new method to isolate large numbers of rabbit osteoclasts and osteoclast-like cells: application to the characterization of serum response element binding proteins during osteoclast differentiation. J BONE MINER RES, 13(11), 1730-8. https://doi.org/10.1359/jbmr.1998.13.11.1730

Vancouver

David JP, Neff L, Chen Y, Rincon M, Horne WC, Baron R. A new method to isolate large numbers of rabbit osteoclasts and osteoclast-like cells: application to the characterization of serum response element binding proteins during osteoclast differentiation. J BONE MINER RES. 1998 Nov 1;13(11):1730-8. https://doi.org/10.1359/jbmr.1998.13.11.1730

Bibtex

@article{f6bfbb7d02424823ab5839788bc18ca9,
title = "A new method to isolate large numbers of rabbit osteoclasts and osteoclast-like cells: application to the characterization of serum response element binding proteins during osteoclast differentiation",
abstract = "We have developed a new method that allows the purification of large numbers of both authentic osteoclasts (OCs) and in vitro differentiated osteoclast-like cells (OCLs) from rabbits. We characterized the OCLs in terms of the expression of different phenotypic markers of OC differentiation and their ability to resorb bone. The method provides a system for performing biochemical and molecular studies of OC differentiation and function in a single species. We used this system to characterize the effect of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) on the expression of proteins that bind to the serum response element (SRE) of the c-fos promoter. We found that OCLs and OCs displayed similar SRE-binding activities, including the serum response factor (SRF). This pattern is established in a time-dependent and cell-specific manner in response to long-term treatment of rabbit bone marrow by 1,25(OH)2D3. Thus, 1,25(OH)2D3 can modulate SRF and/or SRF-related protein. This finding may contribute to understanding the role of c-Fos in the regulation of OC differentiation.",
keywords = "Animals, Animals, Newborn, Blood Proteins, Bone Resorption, Calcitonin, Calcitriol, Cell Differentiation, Cell Separation, Coculture Techniques, Immunohistochemistry, Osteoclasts, Proto-Oncogene Proteins c-fos, Rabbits",
author = "David, {J P} and L Neff and Y Chen and M Rincon and Horne, {W C} and R Baron",
year = "1998",
month = nov,
day = "1",
doi = "10.1359/jbmr.1998.13.11.1730",
language = "English",
volume = "13",
pages = "1730--8",
journal = "J BONE MINER RES",
issn = "0884-0431",
publisher = "Wiley-Blackwell",
number = "11",

}

RIS

TY - JOUR

T1 - A new method to isolate large numbers of rabbit osteoclasts and osteoclast-like cells

T2 - application to the characterization of serum response element binding proteins during osteoclast differentiation

AU - David, J P

AU - Neff, L

AU - Chen, Y

AU - Rincon, M

AU - Horne, W C

AU - Baron, R

PY - 1998/11/1

Y1 - 1998/11/1

N2 - We have developed a new method that allows the purification of large numbers of both authentic osteoclasts (OCs) and in vitro differentiated osteoclast-like cells (OCLs) from rabbits. We characterized the OCLs in terms of the expression of different phenotypic markers of OC differentiation and their ability to resorb bone. The method provides a system for performing biochemical and molecular studies of OC differentiation and function in a single species. We used this system to characterize the effect of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) on the expression of proteins that bind to the serum response element (SRE) of the c-fos promoter. We found that OCLs and OCs displayed similar SRE-binding activities, including the serum response factor (SRF). This pattern is established in a time-dependent and cell-specific manner in response to long-term treatment of rabbit bone marrow by 1,25(OH)2D3. Thus, 1,25(OH)2D3 can modulate SRF and/or SRF-related protein. This finding may contribute to understanding the role of c-Fos in the regulation of OC differentiation.

AB - We have developed a new method that allows the purification of large numbers of both authentic osteoclasts (OCs) and in vitro differentiated osteoclast-like cells (OCLs) from rabbits. We characterized the OCLs in terms of the expression of different phenotypic markers of OC differentiation and their ability to resorb bone. The method provides a system for performing biochemical and molecular studies of OC differentiation and function in a single species. We used this system to characterize the effect of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) on the expression of proteins that bind to the serum response element (SRE) of the c-fos promoter. We found that OCLs and OCs displayed similar SRE-binding activities, including the serum response factor (SRF). This pattern is established in a time-dependent and cell-specific manner in response to long-term treatment of rabbit bone marrow by 1,25(OH)2D3. Thus, 1,25(OH)2D3 can modulate SRF and/or SRF-related protein. This finding may contribute to understanding the role of c-Fos in the regulation of OC differentiation.

KW - Animals

KW - Animals, Newborn

KW - Blood Proteins

KW - Bone Resorption

KW - Calcitonin

KW - Calcitriol

KW - Cell Differentiation

KW - Cell Separation

KW - Coculture Techniques

KW - Immunohistochemistry

KW - Osteoclasts

KW - Proto-Oncogene Proteins c-fos

KW - Rabbits

U2 - 10.1359/jbmr.1998.13.11.1730

DO - 10.1359/jbmr.1998.13.11.1730

M3 - SCORING: Journal article

C2 - 9797482

VL - 13

SP - 1730

EP - 1738

JO - J BONE MINER RES

JF - J BONE MINER RES

SN - 0884-0431

IS - 11

ER -