A new method to isolate large numbers of rabbit osteoclasts and osteoclast-like cells
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A new method to isolate large numbers of rabbit osteoclasts and osteoclast-like cells : application to the characterization of serum response element binding proteins during osteoclast differentiation. / David, J P; Neff, L; Chen, Y; Rincon, M; Horne, W C; Baron, R.
in: J BONE MINER RES, Jahrgang 13, Nr. 11, 01.11.1998, S. 1730-8.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - A new method to isolate large numbers of rabbit osteoclasts and osteoclast-like cells
T2 - application to the characterization of serum response element binding proteins during osteoclast differentiation
AU - David, J P
AU - Neff, L
AU - Chen, Y
AU - Rincon, M
AU - Horne, W C
AU - Baron, R
PY - 1998/11/1
Y1 - 1998/11/1
N2 - We have developed a new method that allows the purification of large numbers of both authentic osteoclasts (OCs) and in vitro differentiated osteoclast-like cells (OCLs) from rabbits. We characterized the OCLs in terms of the expression of different phenotypic markers of OC differentiation and their ability to resorb bone. The method provides a system for performing biochemical and molecular studies of OC differentiation and function in a single species. We used this system to characterize the effect of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) on the expression of proteins that bind to the serum response element (SRE) of the c-fos promoter. We found that OCLs and OCs displayed similar SRE-binding activities, including the serum response factor (SRF). This pattern is established in a time-dependent and cell-specific manner in response to long-term treatment of rabbit bone marrow by 1,25(OH)2D3. Thus, 1,25(OH)2D3 can modulate SRF and/or SRF-related protein. This finding may contribute to understanding the role of c-Fos in the regulation of OC differentiation.
AB - We have developed a new method that allows the purification of large numbers of both authentic osteoclasts (OCs) and in vitro differentiated osteoclast-like cells (OCLs) from rabbits. We characterized the OCLs in terms of the expression of different phenotypic markers of OC differentiation and their ability to resorb bone. The method provides a system for performing biochemical and molecular studies of OC differentiation and function in a single species. We used this system to characterize the effect of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) on the expression of proteins that bind to the serum response element (SRE) of the c-fos promoter. We found that OCLs and OCs displayed similar SRE-binding activities, including the serum response factor (SRF). This pattern is established in a time-dependent and cell-specific manner in response to long-term treatment of rabbit bone marrow by 1,25(OH)2D3. Thus, 1,25(OH)2D3 can modulate SRF and/or SRF-related protein. This finding may contribute to understanding the role of c-Fos in the regulation of OC differentiation.
KW - Animals
KW - Animals, Newborn
KW - Blood Proteins
KW - Bone Resorption
KW - Calcitonin
KW - Calcitriol
KW - Cell Differentiation
KW - Cell Separation
KW - Coculture Techniques
KW - Immunohistochemistry
KW - Osteoclasts
KW - Proto-Oncogene Proteins c-fos
KW - Rabbits
U2 - 10.1359/jbmr.1998.13.11.1730
DO - 10.1359/jbmr.1998.13.11.1730
M3 - SCORING: Journal article
C2 - 9797482
VL - 13
SP - 1730
EP - 1738
JO - J BONE MINER RES
JF - J BONE MINER RES
SN - 0884-0431
IS - 11
ER -