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A Modular Framework for Post-Processing and Analysis of Fluorescence Microscopy Image Sequences of Subcellular Calcium Dynamics

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A Modular Framework for Post-Processing and Analysis of Fluorescence Microscopy Image Sequences of Subcellular Calcium Dynamics. / Schetelig, Daniel; Wolf, Insa; Diercks, Björn-Philipp; Fliegert, Ralf; Guse, Andreas; Schlaefer, Alexander; Werner, Rene.

Bildverarbeitung für die Medizin 2015. ed. / Heinz Handels; Thomas Martin Deserno; Hans-Peter Meinzer; Thomas Tolxdorff. 1. ed. Berlin Heidelberg : Springer, 2015. p. 401-406 (Informatik Aktuell).

Research output: SCORING: Contribution to book/anthology › SCORING: Contribution to collected editions/anthologies › Research › peer-review

Harvard

Schetelig, D, Wolf, I, Diercks, B-P, Fliegert, R, Guse, A, Schlaefer, A & Werner, R 2015, A Modular Framework for Post-Processing and Analysis of Fluorescence Microscopy Image Sequences of Subcellular Calcium Dynamics. in H Handels, TM Deserno, H-P Meinzer & T Tolxdorff (eds), Bildverarbeitung für die Medizin 2015. 1 edn, Informatik Aktuell, Springer, Berlin Heidelberg, pp. 401-406. https://doi.org/10.1007/978-3-662-46224-9_69

APA

Schetelig, D., Wolf, I., Diercks, B-P., Fliegert, R., Guse, A., Schlaefer, A., & Werner, R. (2015). A Modular Framework for Post-Processing and Analysis of Fluorescence Microscopy Image Sequences of Subcellular Calcium Dynamics. In H. Handels, T. M. Deserno, H-P. Meinzer, & T. Tolxdorff (Eds.), Bildverarbeitung für die Medizin 2015 (1 ed., pp. 401-406). (Informatik Aktuell). Springer. https://doi.org/10.1007/978-3-662-46224-9_69

Vancouver

Schetelig D, Wolf I, Diercks B-P, Fliegert R, Guse A, Schlaefer A et al. A Modular Framework for Post-Processing and Analysis of Fluorescence Microscopy Image Sequences of Subcellular Calcium Dynamics. In Handels H, Deserno TM, Meinzer H-P, Tolxdorff T, editors, Bildverarbeitung für die Medizin 2015. 1 ed. Berlin Heidelberg: Springer. 2015. p. 401-406. (Informatik Aktuell). https://doi.org/10.1007/978-3-662-46224-9_69

Bibtex

@inbook{a1cdca70087c473291bd9f9419d099cc,
title = "A Modular Framework for Post-Processing and Analysis of Fluorescence Microscopy Image Sequences of Subcellular Calcium Dynamics",
abstract = "Calcium (Ca2+) signaling is essential for activation of Tlymphocytes and can be understood as fundamental on-switch for the adaptive immune system. The activation is supposed to start by initial spatially and temporally localized Ca2+ signals. Imaging and analysis of these signals require high spatio-temporal resolution fluorescence microscopy – which, in turn, results in the need for an efficient and reliable post-processing and analysis workflow of the acquired image data. Started with a well established but time-consuming post-processing process, we report on our efforts to automatize and optimize it. The efforts led to a modular post-processing and analysis framework, which is presented. In addition, the influence of instances of the main blocks of the framework (e.g. bleaching correction, deconvolution) on Ca2+ dynamics analysis measures is evaluated.",
author = "Daniel Schetelig and Insa Wolf and Bj{\"o}rn-Philipp Diercks and Ralf Fliegert and Andreas Guse and Alexander Schlaefer and Rene Werner",
year = "2015",
doi = "10.1007/978-3-662-46224-9_69",
language = "English",
isbn = "978-3-662-46223-2",
series = "Informatik Aktuell",
publisher = "Springer",
pages = "401--406",
editor = "Heinz Handels and Deserno, {Thomas Martin} and Hans-Peter Meinzer and Thomas Tolxdorff",
booktitle = "Bildverarbeitung f{\"u}r die Medizin 2015",
address = "Germany",
edition = "1",

}

RIS

TY - CHAP

T1 - A Modular Framework for Post-Processing and Analysis of Fluorescence Microscopy Image Sequences of Subcellular Calcium Dynamics

AU - Schetelig, Daniel

AU - Wolf, Insa

AU - Diercks, Björn-Philipp

AU - Fliegert, Ralf

AU - Guse, Andreas

AU - Schlaefer, Alexander

AU - Werner, Rene

PY - 2015

Y1 - 2015

N2 - Calcium (Ca2+) signaling is essential for activation of Tlymphocytes and can be understood as fundamental on-switch for the adaptive immune system. The activation is supposed to start by initial spatially and temporally localized Ca2+ signals. Imaging and analysis of these signals require high spatio-temporal resolution fluorescence microscopy – which, in turn, results in the need for an efficient and reliable post-processing and analysis workflow of the acquired image data. Started with a well established but time-consuming post-processing process, we report on our efforts to automatize and optimize it. The efforts led to a modular post-processing and analysis framework, which is presented. In addition, the influence of instances of the main blocks of the framework (e.g. bleaching correction, deconvolution) on Ca2+ dynamics analysis measures is evaluated.

AB - Calcium (Ca2+) signaling is essential for activation of Tlymphocytes and can be understood as fundamental on-switch for the adaptive immune system. The activation is supposed to start by initial spatially and temporally localized Ca2+ signals. Imaging and analysis of these signals require high spatio-temporal resolution fluorescence microscopy – which, in turn, results in the need for an efficient and reliable post-processing and analysis workflow of the acquired image data. Started with a well established but time-consuming post-processing process, we report on our efforts to automatize and optimize it. The efforts led to a modular post-processing and analysis framework, which is presented. In addition, the influence of instances of the main blocks of the framework (e.g. bleaching correction, deconvolution) on Ca2+ dynamics analysis measures is evaluated.

U2 - 10.1007/978-3-662-46224-9_69

DO - 10.1007/978-3-662-46224-9_69

M3 - SCORING: Contribution to collected editions/anthologies

SN - 978-3-662-46223-2

T3 - Informatik Aktuell

SP - 401

EP - 406

BT - Bildverarbeitung für die Medizin 2015

A2 - Handels, Heinz

A2 - Deserno, Thomas Martin

A2 - Meinzer, Hans-Peter

A2 - Tolxdorff, Thomas

PB - Springer

CY - Berlin Heidelberg

ER -