A modified approach for programmed electrical stimulation in mice: Inducibility of ventricular arrhythmias
Standard
A modified approach for programmed electrical stimulation in mice: Inducibility of ventricular arrhythmias. / Clasen, Lukas; Eickholt, Christian; Angendohr, Stephan; Jungen, Christiane; Shin, Dong-In; Donner, Birgit; Fürnkranz, Alexander; Kelm, Malte; Klöcker, Nikolaj; Meyer, Christian; Makimoto, Hisaki.
In: PLOS ONE, Vol. 13, No. 8, e0201910, 2018.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - A modified approach for programmed electrical stimulation in mice: Inducibility of ventricular arrhythmias
AU - Clasen, Lukas
AU - Eickholt, Christian
AU - Angendohr, Stephan
AU - Jungen, Christiane
AU - Shin, Dong-In
AU - Donner, Birgit
AU - Fürnkranz, Alexander
AU - Kelm, Malte
AU - Klöcker, Nikolaj
AU - Meyer, Christian
AU - Makimoto, Hisaki
PY - 2018
Y1 - 2018
N2 - BACKGROUND: Electrophysiological studies in mice, the prevailing model organism in the field of basic cardiovascular research, are impeded by the low yield of programmed electrical stimulation (PES).OBJECTIVE: To investigate a modified approach for ventricular arrhythmia (VA) induction and a novel scoring system in mice.METHOD: A systematic review of literature on current methods for PES in mice searching the PubMed database revealed that VA inducibility was low and ranged widely (4.6 ± 10.7%). Based on this literature review, a modified PES protocol with 3 to 10 extrastimuli was developed and tested in comparison to the conventional PES protocol using up to 3 extrastimuli in anesthetized wildtype mice (C57BL/6J, n = 12). Induced VA, classified according to the Lambeth Convention, were assessed by established arrhythmia scores as well as a novel arrhythmia score based on VA duration.RESULTS: PES with the modified approach raised both the occurrence and the duration of VA compared to conventional PES (0% vs 50%; novel VA score p = 0.0002). Particularly, coupling of >6 extrastimuli raised the induction of VA. Predominantly, premature ventricular complexes (n = 6) and ventricular tachycardia <1s (n = 4) were observed. Repeated PES after adrenergic stimulation using isoprenaline resulted in enhanced induction of ventricular tachycardia <1s in both protocols.CONCLUSION: Our findings suggest that the presented approach of modified PES enables effective induction and quantification of VA in wildtype mice and may well be suited to document and evaluate detailed VA characteristics in mice.
AB - BACKGROUND: Electrophysiological studies in mice, the prevailing model organism in the field of basic cardiovascular research, are impeded by the low yield of programmed electrical stimulation (PES).OBJECTIVE: To investigate a modified approach for ventricular arrhythmia (VA) induction and a novel scoring system in mice.METHOD: A systematic review of literature on current methods for PES in mice searching the PubMed database revealed that VA inducibility was low and ranged widely (4.6 ± 10.7%). Based on this literature review, a modified PES protocol with 3 to 10 extrastimuli was developed and tested in comparison to the conventional PES protocol using up to 3 extrastimuli in anesthetized wildtype mice (C57BL/6J, n = 12). Induced VA, classified according to the Lambeth Convention, were assessed by established arrhythmia scores as well as a novel arrhythmia score based on VA duration.RESULTS: PES with the modified approach raised both the occurrence and the duration of VA compared to conventional PES (0% vs 50%; novel VA score p = 0.0002). Particularly, coupling of >6 extrastimuli raised the induction of VA. Predominantly, premature ventricular complexes (n = 6) and ventricular tachycardia <1s (n = 4) were observed. Repeated PES after adrenergic stimulation using isoprenaline resulted in enhanced induction of ventricular tachycardia <1s in both protocols.CONCLUSION: Our findings suggest that the presented approach of modified PES enables effective induction and quantification of VA in wildtype mice and may well be suited to document and evaluate detailed VA characteristics in mice.
KW - Animals
KW - Arrhythmias, Cardiac/etiology
KW - Disease Models, Animal
KW - Electric Stimulation/adverse effects
KW - Heart Ventricles/physiopathology
KW - Male
KW - Mice
KW - Mice, Inbred C57BL
KW - Tachycardia, Ventricular/etiology
KW - Ventricular Fibrillation/etiology
KW - Ventricular Flutter/etiology
U2 - 10.1371/journal.pone.0201910
DO - 10.1371/journal.pone.0201910
M3 - SCORING: Journal article
C2 - 30133474
VL - 13
JO - PLOS ONE
JF - PLOS ONE
SN - 1932-6203
IS - 8
M1 - e0201910
ER -