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A modified approach for programmed electrical stimulation in mice: Inducibility of ventricular arrhythmias

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A modified approach for programmed electrical stimulation in mice: Inducibility of ventricular arrhythmias. / Clasen, Lukas; Eickholt, Christian; Angendohr, Stephan; Jungen, Christiane; Shin, Dong-In; Donner, Birgit; Fürnkranz, Alexander; Kelm, Malte; Klöcker, Nikolaj; Meyer, Christian; Makimoto, Hisaki.

in: PLOS ONE, Jahrgang 13, Nr. 8, e0201910, 2018.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Clasen, L, Eickholt, C, Angendohr, S, Jungen, C, Shin, D-I, Donner, B, Fürnkranz, A, Kelm, M, Klöcker, N, Meyer, C & Makimoto, H 2018, 'A modified approach for programmed electrical stimulation in mice: Inducibility of ventricular arrhythmias', PLOS ONE, Jg. 13, Nr. 8, e0201910. https://doi.org/10.1371/journal.pone.0201910

APA

Clasen, L., Eickholt, C., Angendohr, S., Jungen, C., Shin, D-I., Donner, B., Fürnkranz, A., Kelm, M., Klöcker, N., Meyer, C., & Makimoto, H. (2018). A modified approach for programmed electrical stimulation in mice: Inducibility of ventricular arrhythmias. PLOS ONE, 13(8), [e0201910]. https://doi.org/10.1371/journal.pone.0201910

Vancouver

Clasen L, Eickholt C, Angendohr S, Jungen C, Shin D-I, Donner B et al. A modified approach for programmed electrical stimulation in mice: Inducibility of ventricular arrhythmias. PLOS ONE. 2018;13(8). e0201910. https://doi.org/10.1371/journal.pone.0201910

Bibtex

@article{9b61956f9e3a4aa1997a75b2a639c24e,
title = "A modified approach for programmed electrical stimulation in mice: Inducibility of ventricular arrhythmias",
abstract = "BACKGROUND: Electrophysiological studies in mice, the prevailing model organism in the field of basic cardiovascular research, are impeded by the low yield of programmed electrical stimulation (PES).OBJECTIVE: To investigate a modified approach for ventricular arrhythmia (VA) induction and a novel scoring system in mice.METHOD: A systematic review of literature on current methods for PES in mice searching the PubMed database revealed that VA inducibility was low and ranged widely (4.6 ± 10.7%). Based on this literature review, a modified PES protocol with 3 to 10 extrastimuli was developed and tested in comparison to the conventional PES protocol using up to 3 extrastimuli in anesthetized wildtype mice (C57BL/6J, n = 12). Induced VA, classified according to the Lambeth Convention, were assessed by established arrhythmia scores as well as a novel arrhythmia score based on VA duration.RESULTS: PES with the modified approach raised both the occurrence and the duration of VA compared to conventional PES (0% vs 50%; novel VA score p = 0.0002). Particularly, coupling of >6 extrastimuli raised the induction of VA. Predominantly, premature ventricular complexes (n = 6) and ventricular tachycardia <1s (n = 4) were observed. Repeated PES after adrenergic stimulation using isoprenaline resulted in enhanced induction of ventricular tachycardia <1s in both protocols.CONCLUSION: Our findings suggest that the presented approach of modified PES enables effective induction and quantification of VA in wildtype mice and may well be suited to document and evaluate detailed VA characteristics in mice.",
keywords = "Animals, Arrhythmias, Cardiac/etiology, Disease Models, Animal, Electric Stimulation/adverse effects, Heart Ventricles/physiopathology, Male, Mice, Mice, Inbred C57BL, Tachycardia, Ventricular/etiology, Ventricular Fibrillation/etiology, Ventricular Flutter/etiology",
author = "Lukas Clasen and Christian Eickholt and Stephan Angendohr and Christiane Jungen and Dong-In Shin and Birgit Donner and Alexander F{\"u}rnkranz and Malte Kelm and Nikolaj Kl{\"o}cker and Christian Meyer and Hisaki Makimoto",
year = "2018",
doi = "10.1371/journal.pone.0201910",
language = "English",
volume = "13",
journal = "PLOS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "8",

}

RIS

TY - JOUR

T1 - A modified approach for programmed electrical stimulation in mice: Inducibility of ventricular arrhythmias

AU - Clasen, Lukas

AU - Eickholt, Christian

AU - Angendohr, Stephan

AU - Jungen, Christiane

AU - Shin, Dong-In

AU - Donner, Birgit

AU - Fürnkranz, Alexander

AU - Kelm, Malte

AU - Klöcker, Nikolaj

AU - Meyer, Christian

AU - Makimoto, Hisaki

PY - 2018

Y1 - 2018

N2 - BACKGROUND: Electrophysiological studies in mice, the prevailing model organism in the field of basic cardiovascular research, are impeded by the low yield of programmed electrical stimulation (PES).OBJECTIVE: To investigate a modified approach for ventricular arrhythmia (VA) induction and a novel scoring system in mice.METHOD: A systematic review of literature on current methods for PES in mice searching the PubMed database revealed that VA inducibility was low and ranged widely (4.6 ± 10.7%). Based on this literature review, a modified PES protocol with 3 to 10 extrastimuli was developed and tested in comparison to the conventional PES protocol using up to 3 extrastimuli in anesthetized wildtype mice (C57BL/6J, n = 12). Induced VA, classified according to the Lambeth Convention, were assessed by established arrhythmia scores as well as a novel arrhythmia score based on VA duration.RESULTS: PES with the modified approach raised both the occurrence and the duration of VA compared to conventional PES (0% vs 50%; novel VA score p = 0.0002). Particularly, coupling of >6 extrastimuli raised the induction of VA. Predominantly, premature ventricular complexes (n = 6) and ventricular tachycardia <1s (n = 4) were observed. Repeated PES after adrenergic stimulation using isoprenaline resulted in enhanced induction of ventricular tachycardia <1s in both protocols.CONCLUSION: Our findings suggest that the presented approach of modified PES enables effective induction and quantification of VA in wildtype mice and may well be suited to document and evaluate detailed VA characteristics in mice.

AB - BACKGROUND: Electrophysiological studies in mice, the prevailing model organism in the field of basic cardiovascular research, are impeded by the low yield of programmed electrical stimulation (PES).OBJECTIVE: To investigate a modified approach for ventricular arrhythmia (VA) induction and a novel scoring system in mice.METHOD: A systematic review of literature on current methods for PES in mice searching the PubMed database revealed that VA inducibility was low and ranged widely (4.6 ± 10.7%). Based on this literature review, a modified PES protocol with 3 to 10 extrastimuli was developed and tested in comparison to the conventional PES protocol using up to 3 extrastimuli in anesthetized wildtype mice (C57BL/6J, n = 12). Induced VA, classified according to the Lambeth Convention, were assessed by established arrhythmia scores as well as a novel arrhythmia score based on VA duration.RESULTS: PES with the modified approach raised both the occurrence and the duration of VA compared to conventional PES (0% vs 50%; novel VA score p = 0.0002). Particularly, coupling of >6 extrastimuli raised the induction of VA. Predominantly, premature ventricular complexes (n = 6) and ventricular tachycardia <1s (n = 4) were observed. Repeated PES after adrenergic stimulation using isoprenaline resulted in enhanced induction of ventricular tachycardia <1s in both protocols.CONCLUSION: Our findings suggest that the presented approach of modified PES enables effective induction and quantification of VA in wildtype mice and may well be suited to document and evaluate detailed VA characteristics in mice.

KW - Animals

KW - Arrhythmias, Cardiac/etiology

KW - Disease Models, Animal

KW - Electric Stimulation/adverse effects

KW - Heart Ventricles/physiopathology

KW - Male

KW - Mice

KW - Mice, Inbred C57BL

KW - Tachycardia, Ventricular/etiology

KW - Ventricular Fibrillation/etiology

KW - Ventricular Flutter/etiology

U2 - 10.1371/journal.pone.0201910

DO - 10.1371/journal.pone.0201910

M3 - SCORING: Journal article

C2 - 30133474

VL - 13

JO - PLOS ONE

JF - PLOS ONE

SN - 1932-6203

IS - 8

M1 - e0201910

ER -