A flexible and versatile system for multi-color fiber photometry and optogenetic manipulation
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A flexible and versatile system for multi-color fiber photometry and optogenetic manipulation. / Formozov, Andrey; Dieter, Alexander; Wiegert, J Simon.
In: CELL REP METHODS, Vol. 3, No. 3, 27.03.2023, p. 100418.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - A flexible and versatile system for multi-color fiber photometry and optogenetic manipulation
AU - Formozov, Andrey
AU - Dieter, Alexander
AU - Wiegert, J Simon
N1 - © 2023 The Author(s).
PY - 2023/3/27
Y1 - 2023/3/27
N2 - Here, we present simultaneous fiber photometry recordings and optogenetic stimulation based on a multimode fused fiber coupler for both light delivery and collection without the need for dichroic beam splitters. In combination with a multi-color light source and appropriate optical filters, our approach offers remarkable flexibility in experimental design and facilitates the exploration of new molecular tools in vivo at minimal cost. We demonstrate straightforward re-configuration of the setup to operate with green, red, and near-infrared calcium indicators with or without simultaneous optogenetic stimulation and further explore the multi-color photometry capabilities of the system. The ease of assembly, operation, characterization, and customization of this platform holds the potential to foster the development of experimental strategies for multi-color fused fiber photometry combined with optogenetics far beyond its current state.
AB - Here, we present simultaneous fiber photometry recordings and optogenetic stimulation based on a multimode fused fiber coupler for both light delivery and collection without the need for dichroic beam splitters. In combination with a multi-color light source and appropriate optical filters, our approach offers remarkable flexibility in experimental design and facilitates the exploration of new molecular tools in vivo at minimal cost. We demonstrate straightforward re-configuration of the setup to operate with green, red, and near-infrared calcium indicators with or without simultaneous optogenetic stimulation and further explore the multi-color photometry capabilities of the system. The ease of assembly, operation, characterization, and customization of this platform holds the potential to foster the development of experimental strategies for multi-color fused fiber photometry combined with optogenetics far beyond its current state.
U2 - 10.1016/j.crmeth.2023.100418
DO - 10.1016/j.crmeth.2023.100418
M3 - SCORING: Journal article
C2 - 37056369
VL - 3
SP - 100418
JO - CELL REP METHODS
JF - CELL REP METHODS
SN - 2667-2375
IS - 3
ER -