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A flexible and versatile system for multi-color fiber photometry and optogenetic manipulation

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A flexible and versatile system for multi-color fiber photometry and optogenetic manipulation. / Formozov, Andrey; Dieter, Alexander; Wiegert, J Simon.

in: CELL REP METHODS, Jahrgang 3, Nr. 3, 27.03.2023, S. 100418.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Formozov, A, Dieter, A & Wiegert, JS 2023, 'A flexible and versatile system for multi-color fiber photometry and optogenetic manipulation', CELL REP METHODS, Jg. 3, Nr. 3, S. 100418. https://doi.org/10.1016/j.crmeth.2023.100418

APA

Formozov, A., Dieter, A., & Wiegert, J. S. (2023). A flexible and versatile system for multi-color fiber photometry and optogenetic manipulation. CELL REP METHODS, 3(3), 100418. https://doi.org/10.1016/j.crmeth.2023.100418

Vancouver

Formozov A, Dieter A, Wiegert JS. A flexible and versatile system for multi-color fiber photometry and optogenetic manipulation. CELL REP METHODS. 2023 Mär 27;3(3):100418. https://doi.org/10.1016/j.crmeth.2023.100418

Bibtex

@article{40a1492d108142aa888df2381aa00702,
title = "A flexible and versatile system for multi-color fiber photometry and optogenetic manipulation",
abstract = "Here, we present simultaneous fiber photometry recordings and optogenetic stimulation based on a multimode fused fiber coupler for both light delivery and collection without the need for dichroic beam splitters. In combination with a multi-color light source and appropriate optical filters, our approach offers remarkable flexibility in experimental design and facilitates the exploration of new molecular tools in vivo at minimal cost. We demonstrate straightforward re-configuration of the setup to operate with green, red, and near-infrared calcium indicators with or without simultaneous optogenetic stimulation and further explore the multi-color photometry capabilities of the system. The ease of assembly, operation, characterization, and customization of this platform holds the potential to foster the development of experimental strategies for multi-color fused fiber photometry combined with optogenetics far beyond its current state.",
author = "Andrey Formozov and Alexander Dieter and Wiegert, {J Simon}",
note = "{\textcopyright} 2023 The Author(s).",
year = "2023",
month = mar,
day = "27",
doi = "10.1016/j.crmeth.2023.100418",
language = "English",
volume = "3",
pages = "100418",
journal = "CELL REP METHODS",
issn = "2667-2375",
publisher = "Cell Press",
number = "3",

}

RIS

TY - JOUR

T1 - A flexible and versatile system for multi-color fiber photometry and optogenetic manipulation

AU - Formozov, Andrey

AU - Dieter, Alexander

AU - Wiegert, J Simon

N1 - © 2023 The Author(s).

PY - 2023/3/27

Y1 - 2023/3/27

N2 - Here, we present simultaneous fiber photometry recordings and optogenetic stimulation based on a multimode fused fiber coupler for both light delivery and collection without the need for dichroic beam splitters. In combination with a multi-color light source and appropriate optical filters, our approach offers remarkable flexibility in experimental design and facilitates the exploration of new molecular tools in vivo at minimal cost. We demonstrate straightforward re-configuration of the setup to operate with green, red, and near-infrared calcium indicators with or without simultaneous optogenetic stimulation and further explore the multi-color photometry capabilities of the system. The ease of assembly, operation, characterization, and customization of this platform holds the potential to foster the development of experimental strategies for multi-color fused fiber photometry combined with optogenetics far beyond its current state.

AB - Here, we present simultaneous fiber photometry recordings and optogenetic stimulation based on a multimode fused fiber coupler for both light delivery and collection without the need for dichroic beam splitters. In combination with a multi-color light source and appropriate optical filters, our approach offers remarkable flexibility in experimental design and facilitates the exploration of new molecular tools in vivo at minimal cost. We demonstrate straightforward re-configuration of the setup to operate with green, red, and near-infrared calcium indicators with or without simultaneous optogenetic stimulation and further explore the multi-color photometry capabilities of the system. The ease of assembly, operation, characterization, and customization of this platform holds the potential to foster the development of experimental strategies for multi-color fused fiber photometry combined with optogenetics far beyond its current state.

U2 - 10.1016/j.crmeth.2023.100418

DO - 10.1016/j.crmeth.2023.100418

M3 - SCORING: Journal article

C2 - 37056369

VL - 3

SP - 100418

JO - CELL REP METHODS

JF - CELL REP METHODS

SN - 2667-2375

IS - 3

ER -