Research ExplorerPublicationsA Dual-Antigen Enzyme-Linked Immunosorbent Assay Allows the ...

A Dual-Antigen Enzyme-Linked Immunosorbent Assay Allows the Assessment of Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Seroprevalence in a Low-Transmission Setting

Standard

A Dual-Antigen Enzyme-Linked Immunosorbent Assay Allows the Assessment of Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Seroprevalence in a Low-Transmission Setting. / Hicks, Sarah M; Pohl, Kai; Neeman, Teresa; McNamara, Hayley A; Parsons, Kate M; He, Jin-Shu; Ali, Sidra A; Nazir, Samina; Rowntree, Louise C; Nguyen, Thi H O; Kedzierska, Katherine; Doolan, Denise L; Vinuesa, Carola G; Cook, Matthew C; Coatsworth, Nicholas; Myles, Paul S; Kurth, Florian; Sander, Leif E; Mann, Graham J; Gruen, Russell L; George, Amee J; Gardiner, Elizabeth E; Cockburn, Ian A; SARS-CoV-2 Testing in Elective Surgery Collaborators.

In: J INFECT DIS, Vol. 223, No. 1, 04.01.2021, p. 10-14.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Hicks, SM, Pohl, K, Neeman, T, McNamara, HA, Parsons, KM, He, J-S, Ali, SA, Nazir, S, Rowntree, LC, Nguyen, THO, Kedzierska, K, Doolan, DL, Vinuesa, CG, Cook, MC, Coatsworth, N, Myles, PS, Kurth, F, Sander, LE, Mann, GJ, Gruen, RL, George, AJ, Gardiner, EE, Cockburn, IA & SARS-CoV-2 Testing in Elective Surgery Collaborators 2021, 'A Dual-Antigen Enzyme-Linked Immunosorbent Assay Allows the Assessment of Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Seroprevalence in a Low-Transmission Setting', J INFECT DIS, vol. 223, no. 1, pp. 10-14. https://doi.org/10.1093/infdis/jiaa623

APA

Hicks, S. M., Pohl, K., Neeman, T., McNamara, H. A., Parsons, K. M., He, J-S., Ali, S. A., Nazir, S., Rowntree, L. C., Nguyen, T. H. O., Kedzierska, K., Doolan, D. L., Vinuesa, C. G., Cook, M. C., Coatsworth, N., Myles, P. S., Kurth, F., Sander, L. E., Mann, G. J., ... SARS-CoV-2 Testing in Elective Surgery Collaborators (2021). A Dual-Antigen Enzyme-Linked Immunosorbent Assay Allows the Assessment of Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Seroprevalence in a Low-Transmission Setting. J INFECT DIS, 223(1), 10-14. https://doi.org/10.1093/infdis/jiaa623

Vancouver

Hicks SM, Pohl K, Neeman T, McNamara HA, Parsons KM, He J-S et al. A Dual-Antigen Enzyme-Linked Immunosorbent Assay Allows the Assessment of Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Seroprevalence in a Low-Transmission Setting. J INFECT DIS. 2021 Jan 4;223(1):10-14. https://doi.org/10.1093/infdis/jiaa623

Bibtex

@article{aab36ea17ed84b96b9f824b63b08f377,
title = "A Dual-Antigen Enzyme-Linked Immunosorbent Assay Allows the Assessment of Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Seroprevalence in a Low-Transmission Setting",
abstract = "Estimates of seroprevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies have been hampered by inadequate assay sensitivity and specificity. Using an enzyme-linked immunosorbent assay-based approach that combines data about immunoglobulin G responses to both the nucleocapsid and spike receptor binding domain antigens, we show that excellent sensitivity and specificity can be achieved. We used this assay to assess the frequency of virus-specific antibodies in a cohort of elective surgery patients in Australia and estimated seroprevalence in Australia to be 0.28% (95% Confidence Interval, 0-1.15%). These data confirm the low level of transmission of SARS-CoV-2 in Australia before July 2020 and validate the specificity of our assay.",
keywords = "Antibodies, Viral/analysis, Antigens, Viral/immunology, Australia, COVID-19/diagnosis, Coronavirus Nucleocapsid Proteins/immunology, Enzyme-Linked Immunosorbent Assay, Humans, Immunoglobulin G/analysis, Phosphoproteins/immunology, Sensitivity and Specificity, Seroepidemiologic Studies, Spike Glycoprotein, Coronavirus/immunology",
author = "Hicks, {Sarah M} and Kai Pohl and Teresa Neeman and McNamara, {Hayley A} and Parsons, {Kate M} and Jin-Shu He and Ali, {Sidra A} and Samina Nazir and Rowntree, {Louise C} and Nguyen, {Thi H O} and Katherine Kedzierska and Doolan, {Denise L} and Vinuesa, {Carola G} and Cook, {Matthew C} and Nicholas Coatsworth and Myles, {Paul S} and Florian Kurth and Sander, {Leif E} and Mann, {Graham J} and Gruen, {Russell L} and George, {Amee J} and Gardiner, {Elizabeth E} and Cockburn, {Ian A} and {SARS-CoV-2 Testing in Elective Surgery Collaborators}",
note = "{\textcopyright} The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.",
year = "2021",
month = jan,
day = "4",
doi = "10.1093/infdis/jiaa623",
language = "English",
volume = "223",
pages = "10--14",
journal = "J INFECT DIS",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "1",

}

RIS

TY - JOUR

T1 - A Dual-Antigen Enzyme-Linked Immunosorbent Assay Allows the Assessment of Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Seroprevalence in a Low-Transmission Setting

AU - Hicks, Sarah M

AU - Pohl, Kai

AU - Neeman, Teresa

AU - McNamara, Hayley A

AU - Parsons, Kate M

AU - He, Jin-Shu

AU - Ali, Sidra A

AU - Nazir, Samina

AU - Rowntree, Louise C

AU - Nguyen, Thi H O

AU - Kedzierska, Katherine

AU - Doolan, Denise L

AU - Vinuesa, Carola G

AU - Cook, Matthew C

AU - Coatsworth, Nicholas

AU - Myles, Paul S

AU - Kurth, Florian

AU - Sander, Leif E

AU - Mann, Graham J

AU - Gruen, Russell L

AU - George, Amee J

AU - Gardiner, Elizabeth E

AU - Cockburn, Ian A

AU - SARS-CoV-2 Testing in Elective Surgery Collaborators

N1 - © The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.

PY - 2021/1/4

Y1 - 2021/1/4

N2 - Estimates of seroprevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies have been hampered by inadequate assay sensitivity and specificity. Using an enzyme-linked immunosorbent assay-based approach that combines data about immunoglobulin G responses to both the nucleocapsid and spike receptor binding domain antigens, we show that excellent sensitivity and specificity can be achieved. We used this assay to assess the frequency of virus-specific antibodies in a cohort of elective surgery patients in Australia and estimated seroprevalence in Australia to be 0.28% (95% Confidence Interval, 0-1.15%). These data confirm the low level of transmission of SARS-CoV-2 in Australia before July 2020 and validate the specificity of our assay.

AB - Estimates of seroprevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies have been hampered by inadequate assay sensitivity and specificity. Using an enzyme-linked immunosorbent assay-based approach that combines data about immunoglobulin G responses to both the nucleocapsid and spike receptor binding domain antigens, we show that excellent sensitivity and specificity can be achieved. We used this assay to assess the frequency of virus-specific antibodies in a cohort of elective surgery patients in Australia and estimated seroprevalence in Australia to be 0.28% (95% Confidence Interval, 0-1.15%). These data confirm the low level of transmission of SARS-CoV-2 in Australia before July 2020 and validate the specificity of our assay.

KW - Antibodies, Viral/analysis

KW - Antigens, Viral/immunology

KW - Australia

KW - COVID-19/diagnosis

KW - Coronavirus Nucleocapsid Proteins/immunology

KW - Enzyme-Linked Immunosorbent Assay

KW - Humans

KW - Immunoglobulin G/analysis

KW - Phosphoproteins/immunology

KW - Sensitivity and Specificity

KW - Seroepidemiologic Studies

KW - Spike Glycoprotein, Coronavirus/immunology

U2 - 10.1093/infdis/jiaa623

DO - 10.1093/infdis/jiaa623

M3 - SCORING: Journal article

C2 - 33009908

VL - 223

SP - 10

EP - 14

JO - J INFECT DIS

JF - J INFECT DIS

SN - 0022-1899

IS - 1

ER -