A Dual-Antigen Enzyme-Linked Immunosorbent Assay Allows the Assessment of Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Seroprevalence in a Low-Transmission Setting
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A Dual-Antigen Enzyme-Linked Immunosorbent Assay Allows the Assessment of Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Seroprevalence in a Low-Transmission Setting. / Hicks, Sarah M; Pohl, Kai; Neeman, Teresa; McNamara, Hayley A; Parsons, Kate M; He, Jin-Shu; Ali, Sidra A; Nazir, Samina; Rowntree, Louise C; Nguyen, Thi H O; Kedzierska, Katherine; Doolan, Denise L; Vinuesa, Carola G; Cook, Matthew C; Coatsworth, Nicholas; Myles, Paul S; Kurth, Florian; Sander, Leif E; Mann, Graham J; Gruen, Russell L; George, Amee J; Gardiner, Elizabeth E; Cockburn, Ian A; SARS-CoV-2 Testing in Elective Surgery Collaborators.
in: J INFECT DIS, Jahrgang 223, Nr. 1, 04.01.2021, S. 10-14.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - A Dual-Antigen Enzyme-Linked Immunosorbent Assay Allows the Assessment of Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Seroprevalence in a Low-Transmission Setting
AU - Hicks, Sarah M
AU - Pohl, Kai
AU - Neeman, Teresa
AU - McNamara, Hayley A
AU - Parsons, Kate M
AU - He, Jin-Shu
AU - Ali, Sidra A
AU - Nazir, Samina
AU - Rowntree, Louise C
AU - Nguyen, Thi H O
AU - Kedzierska, Katherine
AU - Doolan, Denise L
AU - Vinuesa, Carola G
AU - Cook, Matthew C
AU - Coatsworth, Nicholas
AU - Myles, Paul S
AU - Kurth, Florian
AU - Sander, Leif E
AU - Mann, Graham J
AU - Gruen, Russell L
AU - George, Amee J
AU - Gardiner, Elizabeth E
AU - Cockburn, Ian A
AU - SARS-CoV-2 Testing in Elective Surgery Collaborators
N1 - © The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.
PY - 2021/1/4
Y1 - 2021/1/4
N2 - Estimates of seroprevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies have been hampered by inadequate assay sensitivity and specificity. Using an enzyme-linked immunosorbent assay-based approach that combines data about immunoglobulin G responses to both the nucleocapsid and spike receptor binding domain antigens, we show that excellent sensitivity and specificity can be achieved. We used this assay to assess the frequency of virus-specific antibodies in a cohort of elective surgery patients in Australia and estimated seroprevalence in Australia to be 0.28% (95% Confidence Interval, 0-1.15%). These data confirm the low level of transmission of SARS-CoV-2 in Australia before July 2020 and validate the specificity of our assay.
AB - Estimates of seroprevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies have been hampered by inadequate assay sensitivity and specificity. Using an enzyme-linked immunosorbent assay-based approach that combines data about immunoglobulin G responses to both the nucleocapsid and spike receptor binding domain antigens, we show that excellent sensitivity and specificity can be achieved. We used this assay to assess the frequency of virus-specific antibodies in a cohort of elective surgery patients in Australia and estimated seroprevalence in Australia to be 0.28% (95% Confidence Interval, 0-1.15%). These data confirm the low level of transmission of SARS-CoV-2 in Australia before July 2020 and validate the specificity of our assay.
KW - Antibodies, Viral/analysis
KW - Antigens, Viral/immunology
KW - Australia
KW - COVID-19/diagnosis
KW - Coronavirus Nucleocapsid Proteins/immunology
KW - Enzyme-Linked Immunosorbent Assay
KW - Humans
KW - Immunoglobulin G/analysis
KW - Phosphoproteins/immunology
KW - Sensitivity and Specificity
KW - Seroepidemiologic Studies
KW - Spike Glycoprotein, Coronavirus/immunology
U2 - 10.1093/infdis/jiaa623
DO - 10.1093/infdis/jiaa623
M3 - SCORING: Journal article
C2 - 33009908
VL - 223
SP - 10
EP - 14
JO - J INFECT DIS
JF - J INFECT DIS
SN - 0022-1899
IS - 1
ER -