A direct repeat in the cellular retinol-binding protein type II gene confers differential regulation by RXR and RAR
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A direct repeat in the cellular retinol-binding protein type II gene confers differential regulation by RXR and RAR. / Mangelsdorf, D J; Umesono, K; Kliewer, S A; Borgmeyer, U; Ong, E S; Evans, R M.
In: CELL, Vol. 66, No. 3, 09.08.1991, p. 555-61.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - A direct repeat in the cellular retinol-binding protein type II gene confers differential regulation by RXR and RAR
AU - Mangelsdorf, D J
AU - Umesono, K
AU - Kliewer, S A
AU - Borgmeyer, U
AU - Ong, E S
AU - Evans, R M
PY - 1991/8/9
Y1 - 1991/8/9
N2 - The vitamin A derivative retinoic acid exerts its effects on transcription through two distinct classes of nuclear receptors, the retinoic acid receptor (RAR) and the retinoid X receptor (RXR). We provide evidence that expression of the gene for cellular retinol-binding protein type II (CRBPII), a key protein in the intestinal absorption of vitamin A, is dramatically up-regulated by retinoic acid in the presence of RXR but not RAR. This regulation is conferred through a specific cis element in the CRBPII promoter that contains five nearly perfect tandem repeats of the sequence AGGTCA spaced by a single nucleotide. The discovery of this new RX response element provides a means for distinguishing between the two retinoid receptor systems and suggests that an RXR-mediated pathway exists for modulating vitamin A metabolism.
AB - The vitamin A derivative retinoic acid exerts its effects on transcription through two distinct classes of nuclear receptors, the retinoic acid receptor (RAR) and the retinoid X receptor (RXR). We provide evidence that expression of the gene for cellular retinol-binding protein type II (CRBPII), a key protein in the intestinal absorption of vitamin A, is dramatically up-regulated by retinoic acid in the presence of RXR but not RAR. This regulation is conferred through a specific cis element in the CRBPII promoter that contains five nearly perfect tandem repeats of the sequence AGGTCA spaced by a single nucleotide. The discovery of this new RX response element provides a means for distinguishing between the two retinoid receptor systems and suggests that an RXR-mediated pathway exists for modulating vitamin A metabolism.
KW - Animals
KW - Base Sequence
KW - Carrier Proteins
KW - Cell Line
KW - DNA Mutational Analysis
KW - DNA-Binding Proteins
KW - Gene Expression Regulation
KW - Genes
KW - In Vitro Techniques
KW - Molecular Sequence Data
KW - Oligonucleotides
KW - Promoter Regions, Genetic
KW - Protein Binding
KW - RNA, Messenger
KW - Receptors, Retinoic Acid
KW - Regulatory Sequences, Nucleic Acid
KW - Retinol-Binding Proteins
KW - Retinol-Binding Proteins, Cellular
KW - Transcription Factors
KW - Transcription, Genetic
KW - Tretinoin
M3 - SCORING: Journal article
C2 - 1651173
VL - 66
SP - 555
EP - 561
JO - CELL
JF - CELL
SN - 0092-8674
IS - 3
ER -