A clonotypic Vγ4Jγ1/Vδ5Dδ2Jδ1 innate γδ T-cell population restricted to the CCR6⁺CD27⁻ subset
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A clonotypic Vγ4Jγ1/Vδ5Dδ2Jδ1 innate γδ T-cell population restricted to the CCR6⁺CD27⁻ subset. / Kashani, Elham; Föhse, Lisa; Raha, Solaiman; Sandrock, Inga; Oberdörfer, Linda; Koenecke, Christian; Suerbaum, Sebastian; Weiss, Siegfried; Prinz, Immo.
In: NAT COMMUN, Vol. 6, 13.03.2015, p. 6477.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - A clonotypic Vγ4Jγ1/Vδ5Dδ2Jδ1 innate γδ T-cell population restricted to the CCR6⁺CD27⁻ subset
AU - Kashani, Elham
AU - Föhse, Lisa
AU - Raha, Solaiman
AU - Sandrock, Inga
AU - Oberdörfer, Linda
AU - Koenecke, Christian
AU - Suerbaum, Sebastian
AU - Weiss, Siegfried
AU - Prinz, Immo
PY - 2015/3/13
Y1 - 2015/3/13
N2 - Here we investigate the TCR repertoire of mouse Vγ4(+) γδ T cells in correlation with their developmental origin and homeostasis. By deep sequencing we identify a high frequency of straight Vδ5Dδ2Jδ1 germline rearrangements without P- and N-nucleotides within the otherwise highly diverse Trd repertoire of Vγ4(+) cells. This sequence is infrequent in CCR6(-)CD27(+) cells, but abundant among CCR6(+)CD27(-) γδ T cells. Using an inducible Rag1 knock-in mouse model, we show that γδ T cells generated in the adult thymus rarely contain this germline-rearranged Vδ5Dδ2Jδ1 sequence, confirming its fetal origin. Single-cell analysis and deep sequencing of the Trg locus reveal a dominant CDR3 junctional motif that completes the TCR repertoire of invariant Vγ4(+)Vδ5(+) cells. In conclusion, this study identifies an innate subset of fetal thymus-derived γδ T cells with an invariant Vγ4(+)Vδ5(+) TCR that is restricted to the CCR6(+)CD27(-) subset of γδ T cells.
AB - Here we investigate the TCR repertoire of mouse Vγ4(+) γδ T cells in correlation with their developmental origin and homeostasis. By deep sequencing we identify a high frequency of straight Vδ5Dδ2Jδ1 germline rearrangements without P- and N-nucleotides within the otherwise highly diverse Trd repertoire of Vγ4(+) cells. This sequence is infrequent in CCR6(-)CD27(+) cells, but abundant among CCR6(+)CD27(-) γδ T cells. Using an inducible Rag1 knock-in mouse model, we show that γδ T cells generated in the adult thymus rarely contain this germline-rearranged Vδ5Dδ2Jδ1 sequence, confirming its fetal origin. Single-cell analysis and deep sequencing of the Trg locus reveal a dominant CDR3 junctional motif that completes the TCR repertoire of invariant Vγ4(+)Vδ5(+) cells. In conclusion, this study identifies an innate subset of fetal thymus-derived γδ T cells with an invariant Vγ4(+)Vδ5(+) TCR that is restricted to the CCR6(+)CD27(-) subset of γδ T cells.
KW - Amino Acid Motifs
KW - Amino Acid Sequence
KW - Animals
KW - Cell Movement
KW - Germ-Line Mutation
KW - Green Fluorescent Proteins/metabolism
KW - Immunity, Innate
KW - Mice
KW - Mice, Inbred C57BL
KW - Mice, Transgenic
KW - Molecular Sequence Data
KW - Phenotype
KW - Polymerase Chain Reaction
KW - Receptors, Antigen, T-Cell, gamma-delta/genetics
KW - Receptors, CCR6/metabolism
KW - Sequence Analysis, DNA
KW - Single-Cell Analysis
KW - T-Lymphocyte Subsets/cytology
KW - Thymus Gland/metabolism
KW - Tissue Distribution
KW - Tumor Necrosis Factor Receptor Superfamily, Member 7/metabolism
U2 - 10.1038/ncomms7477
DO - 10.1038/ncomms7477
M3 - SCORING: Journal article
C2 - 25765849
VL - 6
SP - 6477
JO - NAT COMMUN
JF - NAT COMMUN
SN - 2041-1723
ER -