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A clonotypic Vγ4Jγ1/Vδ5Dδ2Jδ1 innate γδ T-cell population restricted to the CCR6⁺CD27⁻ subset

Standard

A clonotypic Vγ4Jγ1/Vδ5Dδ2Jδ1 innate γδ T-cell population restricted to the CCR6⁺CD27⁻ subset. / Kashani, Elham; Föhse, Lisa; Raha, Solaiman; Sandrock, Inga; Oberdörfer, Linda; Koenecke, Christian; Suerbaum, Sebastian; Weiss, Siegfried; Prinz, Immo.

in: NAT COMMUN, Jahrgang 6, 13.03.2015, S. 6477.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Kashani, E, Föhse, L, Raha, S, Sandrock, I, Oberdörfer, L, Koenecke, C, Suerbaum, S, Weiss, S & Prinz, I 2015, 'A clonotypic Vγ4Jγ1/Vδ5Dδ2Jδ1 innate γδ T-cell population restricted to the CCR6⁺CD27⁻ subset', NAT COMMUN, Jg. 6, S. 6477. https://doi.org/10.1038/ncomms7477

APA

Kashani, E., Föhse, L., Raha, S., Sandrock, I., Oberdörfer, L., Koenecke, C., Suerbaum, S., Weiss, S., & Prinz, I. (2015). A clonotypic Vγ4Jγ1/Vδ5Dδ2Jδ1 innate γδ T-cell population restricted to the CCR6⁺CD27⁻ subset. NAT COMMUN, 6, 6477. https://doi.org/10.1038/ncomms7477

Vancouver

Kashani E, Föhse L, Raha S, Sandrock I, Oberdörfer L, Koenecke C et al. A clonotypic Vγ4Jγ1/Vδ5Dδ2Jδ1 innate γδ T-cell population restricted to the CCR6⁺CD27⁻ subset. NAT COMMUN. 2015 Mär 13;6:6477. https://doi.org/10.1038/ncomms7477

Bibtex

@article{9f16125a47e54a14b3a782a28da128f9,
title = "A clonotypic Vγ4Jγ1/Vδ5Dδ2Jδ1 innate γδ T-cell population restricted to the CCR6⁺CD27⁻ subset",
abstract = "Here we investigate the TCR repertoire of mouse Vγ4(+) γδ T cells in correlation with their developmental origin and homeostasis. By deep sequencing we identify a high frequency of straight Vδ5Dδ2Jδ1 germline rearrangements without P- and N-nucleotides within the otherwise highly diverse Trd repertoire of Vγ4(+) cells. This sequence is infrequent in CCR6(-)CD27(+) cells, but abundant among CCR6(+)CD27(-) γδ T cells. Using an inducible Rag1 knock-in mouse model, we show that γδ T cells generated in the adult thymus rarely contain this germline-rearranged Vδ5Dδ2Jδ1 sequence, confirming its fetal origin. Single-cell analysis and deep sequencing of the Trg locus reveal a dominant CDR3 junctional motif that completes the TCR repertoire of invariant Vγ4(+)Vδ5(+) cells. In conclusion, this study identifies an innate subset of fetal thymus-derived γδ T cells with an invariant Vγ4(+)Vδ5(+) TCR that is restricted to the CCR6(+)CD27(-) subset of γδ T cells. ",
keywords = "Amino Acid Motifs, Amino Acid Sequence, Animals, Cell Movement, Germ-Line Mutation, Green Fluorescent Proteins/metabolism, Immunity, Innate, Mice, Mice, Inbred C57BL, Mice, Transgenic, Molecular Sequence Data, Phenotype, Polymerase Chain Reaction, Receptors, Antigen, T-Cell, gamma-delta/genetics, Receptors, CCR6/metabolism, Sequence Analysis, DNA, Single-Cell Analysis, T-Lymphocyte Subsets/cytology, Thymus Gland/metabolism, Tissue Distribution, Tumor Necrosis Factor Receptor Superfamily, Member 7/metabolism",
author = "Elham Kashani and Lisa F{\"o}hse and Solaiman Raha and Inga Sandrock and Linda Oberd{\"o}rfer and Christian Koenecke and Sebastian Suerbaum and Siegfried Weiss and Immo Prinz",
year = "2015",
month = mar,
day = "13",
doi = "10.1038/ncomms7477",
language = "English",
volume = "6",
pages = "6477",
journal = "NAT COMMUN",
issn = "2041-1723",
publisher = "NATURE PUBLISHING GROUP",

}

RIS

TY - JOUR

T1 - A clonotypic Vγ4Jγ1/Vδ5Dδ2Jδ1 innate γδ T-cell population restricted to the CCR6⁺CD27⁻ subset

AU - Kashani, Elham

AU - Föhse, Lisa

AU - Raha, Solaiman

AU - Sandrock, Inga

AU - Oberdörfer, Linda

AU - Koenecke, Christian

AU - Suerbaum, Sebastian

AU - Weiss, Siegfried

AU - Prinz, Immo

PY - 2015/3/13

Y1 - 2015/3/13

N2 - Here we investigate the TCR repertoire of mouse Vγ4(+) γδ T cells in correlation with their developmental origin and homeostasis. By deep sequencing we identify a high frequency of straight Vδ5Dδ2Jδ1 germline rearrangements without P- and N-nucleotides within the otherwise highly diverse Trd repertoire of Vγ4(+) cells. This sequence is infrequent in CCR6(-)CD27(+) cells, but abundant among CCR6(+)CD27(-) γδ T cells. Using an inducible Rag1 knock-in mouse model, we show that γδ T cells generated in the adult thymus rarely contain this germline-rearranged Vδ5Dδ2Jδ1 sequence, confirming its fetal origin. Single-cell analysis and deep sequencing of the Trg locus reveal a dominant CDR3 junctional motif that completes the TCR repertoire of invariant Vγ4(+)Vδ5(+) cells. In conclusion, this study identifies an innate subset of fetal thymus-derived γδ T cells with an invariant Vγ4(+)Vδ5(+) TCR that is restricted to the CCR6(+)CD27(-) subset of γδ T cells.

AB - Here we investigate the TCR repertoire of mouse Vγ4(+) γδ T cells in correlation with their developmental origin and homeostasis. By deep sequencing we identify a high frequency of straight Vδ5Dδ2Jδ1 germline rearrangements without P- and N-nucleotides within the otherwise highly diverse Trd repertoire of Vγ4(+) cells. This sequence is infrequent in CCR6(-)CD27(+) cells, but abundant among CCR6(+)CD27(-) γδ T cells. Using an inducible Rag1 knock-in mouse model, we show that γδ T cells generated in the adult thymus rarely contain this germline-rearranged Vδ5Dδ2Jδ1 sequence, confirming its fetal origin. Single-cell analysis and deep sequencing of the Trg locus reveal a dominant CDR3 junctional motif that completes the TCR repertoire of invariant Vγ4(+)Vδ5(+) cells. In conclusion, this study identifies an innate subset of fetal thymus-derived γδ T cells with an invariant Vγ4(+)Vδ5(+) TCR that is restricted to the CCR6(+)CD27(-) subset of γδ T cells.

KW - Amino Acid Motifs

KW - Amino Acid Sequence

KW - Animals

KW - Cell Movement

KW - Germ-Line Mutation

KW - Green Fluorescent Proteins/metabolism

KW - Immunity, Innate

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Transgenic

KW - Molecular Sequence Data

KW - Phenotype

KW - Polymerase Chain Reaction

KW - Receptors, Antigen, T-Cell, gamma-delta/genetics

KW - Receptors, CCR6/metabolism

KW - Sequence Analysis, DNA

KW - Single-Cell Analysis

KW - T-Lymphocyte Subsets/cytology

KW - Thymus Gland/metabolism

KW - Tissue Distribution

KW - Tumor Necrosis Factor Receptor Superfamily, Member 7/metabolism

U2 - 10.1038/ncomms7477

DO - 10.1038/ncomms7477

M3 - SCORING: Journal article

C2 - 25765849

VL - 6

SP - 6477

JO - NAT COMMUN

JF - NAT COMMUN

SN - 2041-1723

ER -