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3D In vitro model of a functional epidermal permeability barrier from human embryonic stem cells and induced pluripotent stem cells

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3D In vitro model of a functional epidermal permeability barrier from human embryonic stem cells and induced pluripotent stem cells. / Petrova, Anastasia; Celli, Anna; Jacquet, Laureen; Dafou, Dimitra; Crumrine, Debra; Hupe, Melanie; Arno, Matthew; Hobbs, Carl; Cvoro, Aleksandra; Karagiannis, Panagiotis; Devito, Liani; Sun, Richard; Adame, Lillian C; Vaughan, Robert; McGrath, John A; Mauro, Theodora M; Ilic, Dusko.

In: STEM CELL REP, Vol. 2, No. 5, 06.05.2014, p. 675-89.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Petrova, A, Celli, A, Jacquet, L, Dafou, D, Crumrine, D, Hupe, M, Arno, M, Hobbs, C, Cvoro, A, Karagiannis, P, Devito, L, Sun, R, Adame, LC, Vaughan, R, McGrath, JA, Mauro, TM & Ilic, D 2014, '3D In vitro model of a functional epidermal permeability barrier from human embryonic stem cells and induced pluripotent stem cells', STEM CELL REP, vol. 2, no. 5, pp. 675-89. https://doi.org/10.1016/j.stemcr.2014.03.009

APA

Petrova, A., Celli, A., Jacquet, L., Dafou, D., Crumrine, D., Hupe, M., Arno, M., Hobbs, C., Cvoro, A., Karagiannis, P., Devito, L., Sun, R., Adame, L. C., Vaughan, R., McGrath, J. A., Mauro, T. M., & Ilic, D. (2014). 3D In vitro model of a functional epidermal permeability barrier from human embryonic stem cells and induced pluripotent stem cells. STEM CELL REP, 2(5), 675-89. https://doi.org/10.1016/j.stemcr.2014.03.009

Vancouver

Petrova A, Celli A, Jacquet L, Dafou D, Crumrine D, Hupe M et al. 3D In vitro model of a functional epidermal permeability barrier from human embryonic stem cells and induced pluripotent stem cells. STEM CELL REP. 2014 May 6;2(5):675-89. https://doi.org/10.1016/j.stemcr.2014.03.009

Bibtex

@article{beba9d0fb41544638232b305694bc397,
title = "3D In vitro model of a functional epidermal permeability barrier from human embryonic stem cells and induced pluripotent stem cells",
abstract = "Cornification and epidermal barrier defects are associated with a number of clinically diverse skin disorders. However, a suitable in vitro model for studying normal barrier function and barrier defects is still lacking. Here, we demonstrate the generation of human epidermal equivalents (HEEs) from human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). HEEs are structurally similar to native epidermis, with a functional permeability barrier. We exposed a pure population of hESC/iPSC-derived keratinocytes, whose transcriptome corresponds to the gene signature of normal primary human keratinocytes (NHKs), to a sequential high-to-low humidity environment in an air/liquid interface culture. The resulting HEEs had all of the cellular strata of the human epidermis, with skin barrier properties similar to those of normal skin. Such HEEs generated from disease-specific iPSCs will be an invaluable tool not only for dissecting molecular mechanisms that lead to epidermal barrier defects but also for drug development and screening. ",
keywords = "Cell Culture Techniques, Cell Differentiation, Cells, Cultured, Cellular Reprogramming, DNA Methylation, Embryonic Stem Cells/cytology, Epidermis/metabolism, Epithelial-Mesenchymal Transition, Humans, Induced Pluripotent Stem Cells/cytology, Keratin-14/genetics, Keratinocytes/cytology, Models, Biological, Permeability, Principal Component Analysis, Teratoma/pathology, Transcription Factors/genetics, Transcriptome",
author = "Anastasia Petrova and Anna Celli and Laureen Jacquet and Dimitra Dafou and Debra Crumrine and Melanie Hupe and Matthew Arno and Carl Hobbs and Aleksandra Cvoro and Panagiotis Karagiannis and Liani Devito and Richard Sun and Adame, {Lillian C} and Robert Vaughan and McGrath, {John A} and Mauro, {Theodora M} and Dusko Ilic",
year = "2014",
month = may,
day = "6",
doi = "10.1016/j.stemcr.2014.03.009",
language = "English",
volume = "2",
pages = "675--89",
journal = "STEM CELL REP",
issn = "2213-6711",
publisher = "Cell Press",
number = "5",

}

RIS

TY - JOUR

T1 - 3D In vitro model of a functional epidermal permeability barrier from human embryonic stem cells and induced pluripotent stem cells

AU - Petrova, Anastasia

AU - Celli, Anna

AU - Jacquet, Laureen

AU - Dafou, Dimitra

AU - Crumrine, Debra

AU - Hupe, Melanie

AU - Arno, Matthew

AU - Hobbs, Carl

AU - Cvoro, Aleksandra

AU - Karagiannis, Panagiotis

AU - Devito, Liani

AU - Sun, Richard

AU - Adame, Lillian C

AU - Vaughan, Robert

AU - McGrath, John A

AU - Mauro, Theodora M

AU - Ilic, Dusko

PY - 2014/5/6

Y1 - 2014/5/6

N2 - Cornification and epidermal barrier defects are associated with a number of clinically diverse skin disorders. However, a suitable in vitro model for studying normal barrier function and barrier defects is still lacking. Here, we demonstrate the generation of human epidermal equivalents (HEEs) from human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). HEEs are structurally similar to native epidermis, with a functional permeability barrier. We exposed a pure population of hESC/iPSC-derived keratinocytes, whose transcriptome corresponds to the gene signature of normal primary human keratinocytes (NHKs), to a sequential high-to-low humidity environment in an air/liquid interface culture. The resulting HEEs had all of the cellular strata of the human epidermis, with skin barrier properties similar to those of normal skin. Such HEEs generated from disease-specific iPSCs will be an invaluable tool not only for dissecting molecular mechanisms that lead to epidermal barrier defects but also for drug development and screening.

AB - Cornification and epidermal barrier defects are associated with a number of clinically diverse skin disorders. However, a suitable in vitro model for studying normal barrier function and barrier defects is still lacking. Here, we demonstrate the generation of human epidermal equivalents (HEEs) from human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). HEEs are structurally similar to native epidermis, with a functional permeability barrier. We exposed a pure population of hESC/iPSC-derived keratinocytes, whose transcriptome corresponds to the gene signature of normal primary human keratinocytes (NHKs), to a sequential high-to-low humidity environment in an air/liquid interface culture. The resulting HEEs had all of the cellular strata of the human epidermis, with skin barrier properties similar to those of normal skin. Such HEEs generated from disease-specific iPSCs will be an invaluable tool not only for dissecting molecular mechanisms that lead to epidermal barrier defects but also for drug development and screening.

KW - Cell Culture Techniques

KW - Cell Differentiation

KW - Cells, Cultured

KW - Cellular Reprogramming

KW - DNA Methylation

KW - Embryonic Stem Cells/cytology

KW - Epidermis/metabolism

KW - Epithelial-Mesenchymal Transition

KW - Humans

KW - Induced Pluripotent Stem Cells/cytology

KW - Keratin-14/genetics

KW - Keratinocytes/cytology

KW - Models, Biological

KW - Permeability

KW - Principal Component Analysis

KW - Teratoma/pathology

KW - Transcription Factors/genetics

KW - Transcriptome

U2 - 10.1016/j.stemcr.2014.03.009

DO - 10.1016/j.stemcr.2014.03.009

M3 - SCORING: Journal article

C2 - 24936454

VL - 2

SP - 675

EP - 689

JO - STEM CELL REP

JF - STEM CELL REP

SN - 2213-6711

IS - 5

ER -