3D In vitro model of a functional epidermal permeability barrier from human embryonic stem cells and induced pluripotent stem cells
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3D In vitro model of a functional epidermal permeability barrier from human embryonic stem cells and induced pluripotent stem cells. / Petrova, Anastasia; Celli, Anna; Jacquet, Laureen; Dafou, Dimitra; Crumrine, Debra; Hupe, Melanie; Arno, Matthew; Hobbs, Carl; Cvoro, Aleksandra; Karagiannis, Panagiotis; Devito, Liani; Sun, Richard; Adame, Lillian C; Vaughan, Robert; McGrath, John A; Mauro, Theodora M; Ilic, Dusko.
in: STEM CELL REP, Jahrgang 2, Nr. 5, 06.05.2014, S. 675-89.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - 3D In vitro model of a functional epidermal permeability barrier from human embryonic stem cells and induced pluripotent stem cells
AU - Petrova, Anastasia
AU - Celli, Anna
AU - Jacquet, Laureen
AU - Dafou, Dimitra
AU - Crumrine, Debra
AU - Hupe, Melanie
AU - Arno, Matthew
AU - Hobbs, Carl
AU - Cvoro, Aleksandra
AU - Karagiannis, Panagiotis
AU - Devito, Liani
AU - Sun, Richard
AU - Adame, Lillian C
AU - Vaughan, Robert
AU - McGrath, John A
AU - Mauro, Theodora M
AU - Ilic, Dusko
PY - 2014/5/6
Y1 - 2014/5/6
N2 - Cornification and epidermal barrier defects are associated with a number of clinically diverse skin disorders. However, a suitable in vitro model for studying normal barrier function and barrier defects is still lacking. Here, we demonstrate the generation of human epidermal equivalents (HEEs) from human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). HEEs are structurally similar to native epidermis, with a functional permeability barrier. We exposed a pure population of hESC/iPSC-derived keratinocytes, whose transcriptome corresponds to the gene signature of normal primary human keratinocytes (NHKs), to a sequential high-to-low humidity environment in an air/liquid interface culture. The resulting HEEs had all of the cellular strata of the human epidermis, with skin barrier properties similar to those of normal skin. Such HEEs generated from disease-specific iPSCs will be an invaluable tool not only for dissecting molecular mechanisms that lead to epidermal barrier defects but also for drug development and screening.
AB - Cornification and epidermal barrier defects are associated with a number of clinically diverse skin disorders. However, a suitable in vitro model for studying normal barrier function and barrier defects is still lacking. Here, we demonstrate the generation of human epidermal equivalents (HEEs) from human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). HEEs are structurally similar to native epidermis, with a functional permeability barrier. We exposed a pure population of hESC/iPSC-derived keratinocytes, whose transcriptome corresponds to the gene signature of normal primary human keratinocytes (NHKs), to a sequential high-to-low humidity environment in an air/liquid interface culture. The resulting HEEs had all of the cellular strata of the human epidermis, with skin barrier properties similar to those of normal skin. Such HEEs generated from disease-specific iPSCs will be an invaluable tool not only for dissecting molecular mechanisms that lead to epidermal barrier defects but also for drug development and screening.
KW - Cell Culture Techniques
KW - Cell Differentiation
KW - Cells, Cultured
KW - Cellular Reprogramming
KW - DNA Methylation
KW - Embryonic Stem Cells/cytology
KW - Epidermis/metabolism
KW - Epithelial-Mesenchymal Transition
KW - Humans
KW - Induced Pluripotent Stem Cells/cytology
KW - Keratin-14/genetics
KW - Keratinocytes/cytology
KW - Models, Biological
KW - Permeability
KW - Principal Component Analysis
KW - Teratoma/pathology
KW - Transcription Factors/genetics
KW - Transcriptome
U2 - 10.1016/j.stemcr.2014.03.009
DO - 10.1016/j.stemcr.2014.03.009
M3 - SCORING: Journal article
C2 - 24936454
VL - 2
SP - 675
EP - 689
JO - STEM CELL REP
JF - STEM CELL REP
SN - 2213-6711
IS - 5
ER -