Vasoactive diadenosine polyphosphates in human placenta
Standard
Vasoactive diadenosine polyphosphates in human placenta : possible candidates in the pathophysiology of pre-eclampsia? / Jankowski, J; Yoon, M S; Stephan, N; Zidek, W; Schlüter, H.
in: J HYPERTENS, Jahrgang 19, Nr. 3 Pt 2, 03.2001, S. 567-73.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Vasoactive diadenosine polyphosphates in human placenta
T2 - possible candidates in the pathophysiology of pre-eclampsia?
AU - Jankowski, J
AU - Yoon, M S
AU - Stephan, N
AU - Zidek, W
AU - Schlüter, H
PY - 2001/3
Y1 - 2001/3
N2 - BACKGROUND: One hypothesis of the pathophysiology of pre-eclampsia is that placentally derived, yet unidentified, vasoactive factors are released into the maternal circulation, causing hypertension.OBJECTIVE: To determine if diadenosine polyphosphates, new potent vasoconstrictors, are present in human placenta.METHODS AND RESULTS: Human placental tissue was homogenated and fractionated by size-exclusion chromatography, affinity chromatography, anion-exchange chromatography and reversed-phase chromatography. In fractions purified to homogeneity, diadenosine diphosphate, diadenosine triphosphate, diadenosine tetraphosphate, diadenosine pentaphosphate, diadenosine hexaphosphate and diadenosine heptaphosphate were identified by matrix-assisted laser desorption/ionization mass spectrometry, retention-time comparison and enzymatic cleavage analysis.CONCLUSIONS: The presence of diadenosine polyphosphates in human placenta makes them possible candidates for involvement in the pathophysiology of pre-eclampsia. However, their contribution to the pathophysiology of eclampsia requires substantiation in further studies.
AB - BACKGROUND: One hypothesis of the pathophysiology of pre-eclampsia is that placentally derived, yet unidentified, vasoactive factors are released into the maternal circulation, causing hypertension.OBJECTIVE: To determine if diadenosine polyphosphates, new potent vasoconstrictors, are present in human placenta.METHODS AND RESULTS: Human placental tissue was homogenated and fractionated by size-exclusion chromatography, affinity chromatography, anion-exchange chromatography and reversed-phase chromatography. In fractions purified to homogeneity, diadenosine diphosphate, diadenosine triphosphate, diadenosine tetraphosphate, diadenosine pentaphosphate, diadenosine hexaphosphate and diadenosine heptaphosphate were identified by matrix-assisted laser desorption/ionization mass spectrometry, retention-time comparison and enzymatic cleavage analysis.CONCLUSIONS: The presence of diadenosine polyphosphates in human placenta makes them possible candidates for involvement in the pathophysiology of pre-eclampsia. However, their contribution to the pathophysiology of eclampsia requires substantiation in further studies.
KW - Blood Vessels
KW - Chromatography
KW - Dinucleoside Phosphates
KW - Female
KW - Humans
KW - Placenta
KW - Pre-Eclampsia
KW - Pregnancy
KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
M3 - SCORING: Journal article
C2 - 11327631
VL - 19
SP - 567
EP - 573
JO - J HYPERTENS
JF - J HYPERTENS
SN - 0263-6352
IS - 3 Pt 2
ER -