The specification of sympathetic neurotransmitter phenotype depends on gp130 cytokine receptor signaling
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The specification of sympathetic neurotransmitter phenotype depends on gp130 cytokine receptor signaling. / Geissen, M; Heller, S; Pennica, D; Ernsberger, U; Rohrer, H.
in: DEVELOPMENT, Jahrgang 125, Nr. 23, 12.1998, S. 4791-801.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - The specification of sympathetic neurotransmitter phenotype depends on gp130 cytokine receptor signaling
AU - Geissen, M
AU - Heller, S
AU - Pennica, D
AU - Ernsberger, U
AU - Rohrer, H
PY - 1998/12
Y1 - 1998/12
N2 - Sympathetic ganglia are composed of noradrenergic and cholinergic neurons. The differentiation of cholinergic sympathetic neurons is characterized by the expression of choline acetyltransferase (ChAT) and vasoactive intestinal peptide (VIP), induced in vitro by a subfamily of cytokines, including LIF, CNTF, GPA, OSM and cardiotrophin-1 (CT-1). To interfere with the function of these neuropoietic cytokines in vivo, antisense RNA for gp130, the common signal-transducing receptor subunit for neuropoietic cytokines, was expressed in chick sympathetic neurons, using retroviral vectors. A strong reduction in the number of VIP-expressing cells, but not of cells expressing ChAT or the adrenergic marker tyrosine hydroxylase (TH), was observed. These results reveal a physiological role of neuropoietic cytokines for the control of VIP expression during the development of cholinergic sympathetic neurons.
AB - Sympathetic ganglia are composed of noradrenergic and cholinergic neurons. The differentiation of cholinergic sympathetic neurons is characterized by the expression of choline acetyltransferase (ChAT) and vasoactive intestinal peptide (VIP), induced in vitro by a subfamily of cytokines, including LIF, CNTF, GPA, OSM and cardiotrophin-1 (CT-1). To interfere with the function of these neuropoietic cytokines in vivo, antisense RNA for gp130, the common signal-transducing receptor subunit for neuropoietic cytokines, was expressed in chick sympathetic neurons, using retroviral vectors. A strong reduction in the number of VIP-expressing cells, but not of cells expressing ChAT or the adrenergic marker tyrosine hydroxylase (TH), was observed. These results reveal a physiological role of neuropoietic cytokines for the control of VIP expression during the development of cholinergic sympathetic neurons.
KW - Amino Acid Sequence
KW - Animals
KW - Antigens, CD/chemistry
KW - Cells, Cultured
KW - Chick Embryo
KW - Choline O-Acetyltransferase/genetics
KW - Cytokine Receptor gp130
KW - Cytokines/physiology
KW - Ganglia, Sympathetic/cytology
KW - Gene Expression Regulation
KW - Gene Expression Regulation, Developmental
KW - Humans
KW - Membrane Glycoproteins/chemistry
KW - Molecular Sequence Data
KW - Neurons/classification
KW - Phenotype
KW - RNA, Antisense
KW - Receptors, Cytokine/physiology
KW - Recombinant Proteins/biosynthesis
KW - Retroviridae
KW - Sequence Alignment
KW - Sequence Homology, Amino Acid
KW - Signal Transduction
KW - Transfection
KW - Tyrosine 3-Monooxygenase/genetics
KW - Vasoactive Intestinal Peptide/genetics
M3 - SCORING: Journal article
C2 - 9806927
VL - 125
SP - 4791
EP - 4801
JO - DEVELOPMENT
JF - DEVELOPMENT
SN - 0950-1991
IS - 23
ER -