The nucleus acts as a ruler tailoring cell responses to spatial constraints
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The nucleus acts as a ruler tailoring cell responses to spatial constraints. / Lomakin, A J; Cattin, C J; Cuvelier, D; Alraies, Z; Molina, M; Nader, G P F; Srivastava, N; Sáez, P J; Garcia-Arcos, J M; Zhitnyak, I Y; Bhargava, A; Driscoll, M K; Welf, E S; Fiolka, R; Petrie, R J; De Silva, N S; González-Granado, J M; Manel, N; Lennon-Duménil, A M; Müller, D J; Piel, M.
in: SCIENCE, Jahrgang 370, Nr. 6514, 16.10.2020.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - The nucleus acts as a ruler tailoring cell responses to spatial constraints
AU - Lomakin, A J
AU - Cattin, C J
AU - Cuvelier, D
AU - Alraies, Z
AU - Molina, M
AU - Nader, G P F
AU - Srivastava, N
AU - Sáez, P J
AU - Garcia-Arcos, J M
AU - Zhitnyak, I Y
AU - Bhargava, A
AU - Driscoll, M K
AU - Welf, E S
AU - Fiolka, R
AU - Petrie, R J
AU - De Silva, N S
AU - González-Granado, J M
AU - Manel, N
AU - Lennon-Duménil, A M
AU - Müller, D J
AU - Piel, M
N1 - Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
PY - 2020/10/16
Y1 - 2020/10/16
N2 - The microscopic environment inside a metazoan organism is highly crowded. Whether individual cells can tailor their behavior to the limited space remains unclear. In this study, we found that cells measure the degree of spatial confinement by using their largest and stiffest organelle, the nucleus. Cell confinement below a resting nucleus size deforms the nucleus, which expands and stretches its envelope. This activates signaling to the actomyosin cortex via nuclear envelope stretch-sensitive proteins, up-regulating cell contractility. We established that the tailored contractile response constitutes a nuclear ruler-based signaling pathway involved in migratory cell behaviors. Cells rely on the nuclear ruler to modulate the motive force that enables their passage through restrictive pores in complex three-dimensional environments, a process relevant to cancer cell invasion, immune responses, and embryonic development.
AB - The microscopic environment inside a metazoan organism is highly crowded. Whether individual cells can tailor their behavior to the limited space remains unclear. In this study, we found that cells measure the degree of spatial confinement by using their largest and stiffest organelle, the nucleus. Cell confinement below a resting nucleus size deforms the nucleus, which expands and stretches its envelope. This activates signaling to the actomyosin cortex via nuclear envelope stretch-sensitive proteins, up-regulating cell contractility. We established that the tailored contractile response constitutes a nuclear ruler-based signaling pathway involved in migratory cell behaviors. Cells rely on the nuclear ruler to modulate the motive force that enables their passage through restrictive pores in complex three-dimensional environments, a process relevant to cancer cell invasion, immune responses, and embryonic development.
KW - Actomyosin/metabolism
KW - Animals
KW - Cell Movement
KW - Embryonic Development
KW - HeLa Cells
KW - Humans
KW - Mechanotransduction, Cellular
KW - Mice
KW - Myosin Heavy Chains/metabolism
KW - Neoplasm Invasiveness
KW - Neoplasms/pathology
KW - Nuclear Envelope/physiology
U2 - 10.1126/science.aba2894
DO - 10.1126/science.aba2894
M3 - SCORING: Journal article
C2 - 33060332
VL - 370
JO - SCIENCE
JF - SCIENCE
SN - 0036-8075
IS - 6514
ER -