The neuropeptide head activator is a high-affinity ligand for the orphan G-protein-coupled receptor GPR37.
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The neuropeptide head activator is a high-affinity ligand for the orphan G-protein-coupled receptor GPR37. / Rezgaoui, Meriem; Süsens, Ute; Ignatov, Atanas; Gelderblom, Mathias; Glassmeier, Günter; Franke, Inga; Urny, Jens; Imai, Yuzuru; Takahashi, Ryosuke; Schaller, H Chica.
in: J CELL SCI, Jahrgang 119, Nr. 3, 3, 2006, S. 542-549.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - The neuropeptide head activator is a high-affinity ligand for the orphan G-protein-coupled receptor GPR37.
AU - Rezgaoui, Meriem
AU - Süsens, Ute
AU - Ignatov, Atanas
AU - Gelderblom, Mathias
AU - Glassmeier, Günter
AU - Franke, Inga
AU - Urny, Jens
AU - Imai, Yuzuru
AU - Takahashi, Ryosuke
AU - Schaller, H Chica
PY - 2006
Y1 - 2006
N2 - The neuropeptide head activator (HA) is a mitogen for mammalian cell lines of neuronal or neuroendocrine origin. HA signalling is mediated by a G-protein-coupled receptor (GPCR). Orphan GPCRs with homology to peptide receptors were screened for HA interaction. Electrophysiological recordings in frog oocytes and in mammalian cell lines as well as Ca(2+) mobilisation assays revealed nanomolar affinities of HA to GPR37. HA signal transduction through GPR37 was mediated by an inhibitory G protein and required Ca(2+) influx through a channel of the transient receptor potential (TRP) family. It also required activation of Ca(2+)-dependent calmodulin kinase and phosphoinositide 3-kinase. Respective inhibitors blocked HA signalling and HA-induced mitosis in GPR37-expressing cells. HA treatment resulted in internalisation of GPR37. Overexpression of GPR37 led to aggregate formation, retention of the receptor in the cytoplasm and low survival rates of transfected cells, confirming the notion that misfolded GPR37 contributes to cell death, as observed in Parkinson's disease.
AB - The neuropeptide head activator (HA) is a mitogen for mammalian cell lines of neuronal or neuroendocrine origin. HA signalling is mediated by a G-protein-coupled receptor (GPCR). Orphan GPCRs with homology to peptide receptors were screened for HA interaction. Electrophysiological recordings in frog oocytes and in mammalian cell lines as well as Ca(2+) mobilisation assays revealed nanomolar affinities of HA to GPR37. HA signal transduction through GPR37 was mediated by an inhibitory G protein and required Ca(2+) influx through a channel of the transient receptor potential (TRP) family. It also required activation of Ca(2+)-dependent calmodulin kinase and phosphoinositide 3-kinase. Respective inhibitors blocked HA signalling and HA-induced mitosis in GPR37-expressing cells. HA treatment resulted in internalisation of GPR37. Overexpression of GPR37 led to aggregate formation, retention of the receptor in the cytoplasm and low survival rates of transfected cells, confirming the notion that misfolded GPR37 contributes to cell death, as observed in Parkinson's disease.
M3 - SCORING: Zeitschriftenaufsatz
VL - 119
SP - 542
EP - 549
JO - J CELL SCI
JF - J CELL SCI
SN - 0021-9533
IS - 3
M1 - 3
ER -