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The natural HLA ligandome of glioblastoma stem-like cells

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The natural HLA ligandome of glioblastoma stem-like cells : antigen discovery for T cell-based immunotherapy. / Neidert, Marian Christoph; Kowalewski, Daniel Johannes; Silginer, Manuela; Kapolou, Konstantina; Backert, Linus; Freudenmann, Lena Katharina; Peper, Janet Kerstin; Marcu, Ana; Wang, Sophie Shih-Yüng; Walz, Juliane Sarah; Wolpert, Fabian; Rammensee, Hans-Georg; Henschler, Reinhard; Lamszus, Katrin; Westphal, Manfred; Roth, Patrick; Regli, Luca; Stevanović, Stefan; Weller, Michael; Eisele, Günter.

in: ACTA NEUROPATHOL, Jahrgang 135, Nr. 6, 06.2018, S. 923-938.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Neidert, MC, Kowalewski, DJ, Silginer, M, Kapolou, K, Backert, L, Freudenmann, LK, Peper, JK, Marcu, A, Wang, SS-Y, Walz, JS, Wolpert, F, Rammensee, H-G, Henschler, R, Lamszus, K, Westphal, M, Roth, P, Regli, L, Stevanović, S, Weller, M & Eisele, G 2018, 'The natural HLA ligandome of glioblastoma stem-like cells: antigen discovery for T cell-based immunotherapy', ACTA NEUROPATHOL, Jg. 135, Nr. 6, S. 923-938. https://doi.org/10.1007/s00401-018-1836-9

APA

Neidert, M. C., Kowalewski, D. J., Silginer, M., Kapolou, K., Backert, L., Freudenmann, L. K., Peper, J. K., Marcu, A., Wang, S. S-Y., Walz, J. S., Wolpert, F., Rammensee, H-G., Henschler, R., Lamszus, K., Westphal, M., Roth, P., Regli, L., Stevanović, S., Weller, M., & Eisele, G. (2018). The natural HLA ligandome of glioblastoma stem-like cells: antigen discovery for T cell-based immunotherapy. ACTA NEUROPATHOL, 135(6), 923-938. https://doi.org/10.1007/s00401-018-1836-9

Vancouver

Neidert MC, Kowalewski DJ, Silginer M, Kapolou K, Backert L, Freudenmann LK et al. The natural HLA ligandome of glioblastoma stem-like cells: antigen discovery for T cell-based immunotherapy. ACTA NEUROPATHOL. 2018 Jun;135(6):923-938. https://doi.org/10.1007/s00401-018-1836-9

Bibtex

@article{af97b785fbab49b288b3f8a679326a18,
title = "The natural HLA ligandome of glioblastoma stem-like cells: antigen discovery for T cell-based immunotherapy",
abstract = "Glioblastoma is the most frequent malignant primary brain tumor. In a hierarchical tumor model, glioblastoma stem-like cells (GSC) play a major role in tumor initiation and maintenance as well as in therapy resistance and recurrence. Thus, targeting this cellular subset may be key to effective immunotherapy. Here, we present a mass spectrometry-based analysis of HLA-presented peptidomes of GSC and glioblastoma patient specimens. Based on the analysis of patient samples (n = 9) and GSC (n = 3), we performed comparative HLA peptidome profiling against a dataset of normal human tissues. Using this immunopeptidome-centric approach we could clearly delineate a subset of naturally presented, GSC-associated HLA ligands, which might serve as highly specific targets for T cell-based immunotherapy. In total, we identified 17 antigens represented by 41 different HLA ligands showing natural and exclusive presentation both on GSC and patient samples. Importantly, in vitro immunogenicity and antigen-specific target cell killing assays suggest these peptides to be epitopes of functional CD8+ T cell responses, thus rendering them prime candidates for antigen-specific immunotherapy of glioblastoma.",
author = "Neidert, {Marian Christoph} and Kowalewski, {Daniel Johannes} and Manuela Silginer and Konstantina Kapolou and Linus Backert and Freudenmann, {Lena Katharina} and Peper, {Janet Kerstin} and Ana Marcu and Wang, {Sophie Shih-Y{\"u}ng} and Walz, {Juliane Sarah} and Fabian Wolpert and Hans-Georg Rammensee and Reinhard Henschler and Katrin Lamszus and Manfred Westphal and Patrick Roth and Luca Regli and Stefan Stevanovi{\'c} and Michael Weller and G{\"u}nter Eisele",
year = "2018",
month = jun,
doi = "10.1007/s00401-018-1836-9",
language = "English",
volume = "135",
pages = "923--938",
journal = "ACTA NEUROPATHOL",
issn = "0001-6322",
publisher = "Springer",
number = "6",

}

RIS

TY - JOUR

T1 - The natural HLA ligandome of glioblastoma stem-like cells

T2 - antigen discovery for T cell-based immunotherapy

AU - Neidert, Marian Christoph

AU - Kowalewski, Daniel Johannes

AU - Silginer, Manuela

AU - Kapolou, Konstantina

AU - Backert, Linus

AU - Freudenmann, Lena Katharina

AU - Peper, Janet Kerstin

AU - Marcu, Ana

AU - Wang, Sophie Shih-Yüng

AU - Walz, Juliane Sarah

AU - Wolpert, Fabian

AU - Rammensee, Hans-Georg

AU - Henschler, Reinhard

AU - Lamszus, Katrin

AU - Westphal, Manfred

AU - Roth, Patrick

AU - Regli, Luca

AU - Stevanović, Stefan

AU - Weller, Michael

AU - Eisele, Günter

PY - 2018/6

Y1 - 2018/6

N2 - Glioblastoma is the most frequent malignant primary brain tumor. In a hierarchical tumor model, glioblastoma stem-like cells (GSC) play a major role in tumor initiation and maintenance as well as in therapy resistance and recurrence. Thus, targeting this cellular subset may be key to effective immunotherapy. Here, we present a mass spectrometry-based analysis of HLA-presented peptidomes of GSC and glioblastoma patient specimens. Based on the analysis of patient samples (n = 9) and GSC (n = 3), we performed comparative HLA peptidome profiling against a dataset of normal human tissues. Using this immunopeptidome-centric approach we could clearly delineate a subset of naturally presented, GSC-associated HLA ligands, which might serve as highly specific targets for T cell-based immunotherapy. In total, we identified 17 antigens represented by 41 different HLA ligands showing natural and exclusive presentation both on GSC and patient samples. Importantly, in vitro immunogenicity and antigen-specific target cell killing assays suggest these peptides to be epitopes of functional CD8+ T cell responses, thus rendering them prime candidates for antigen-specific immunotherapy of glioblastoma.

AB - Glioblastoma is the most frequent malignant primary brain tumor. In a hierarchical tumor model, glioblastoma stem-like cells (GSC) play a major role in tumor initiation and maintenance as well as in therapy resistance and recurrence. Thus, targeting this cellular subset may be key to effective immunotherapy. Here, we present a mass spectrometry-based analysis of HLA-presented peptidomes of GSC and glioblastoma patient specimens. Based on the analysis of patient samples (n = 9) and GSC (n = 3), we performed comparative HLA peptidome profiling against a dataset of normal human tissues. Using this immunopeptidome-centric approach we could clearly delineate a subset of naturally presented, GSC-associated HLA ligands, which might serve as highly specific targets for T cell-based immunotherapy. In total, we identified 17 antigens represented by 41 different HLA ligands showing natural and exclusive presentation both on GSC and patient samples. Importantly, in vitro immunogenicity and antigen-specific target cell killing assays suggest these peptides to be epitopes of functional CD8+ T cell responses, thus rendering them prime candidates for antigen-specific immunotherapy of glioblastoma.

U2 - 10.1007/s00401-018-1836-9

DO - 10.1007/s00401-018-1836-9

M3 - SCORING: Journal article

C2 - 29557506

VL - 135

SP - 923

EP - 938

JO - ACTA NEUROPATHOL

JF - ACTA NEUROPATHOL

SN - 0001-6322

IS - 6

ER -