The murine AE4 promoter predominantly drives type B intercalated cell specific transcription.
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The murine AE4 promoter predominantly drives type B intercalated cell specific transcription. / Hentschke, Moritz; Hentschke, Suna; Borgmeyer, Uwe; Hübner, Christian; Kurth, Ingo.
in: HISTOCHEM CELL BIOL, 2009.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - The murine AE4 promoter predominantly drives type B intercalated cell specific transcription.
AU - Hentschke, Moritz
AU - Hentschke, Suna
AU - Borgmeyer, Uwe
AU - Hübner, Christian
AU - Kurth, Ingo
PY - 2009
Y1 - 2009
N2 - AE4 is an anion exchanger almost exclusively expressed in the collecting ducts of the kidney. This very restricted expression prompted us to analyze its transcription in more detail. 5' RACE yielded alternative transcriptional start sites that are predicted to code for N-terminal protein variants. Comparison of the 5' genomic sequence between species identified a transcriptionally active region with three conserved spans. In transgenic mice beta-galactosidase expression driven by this fragment resembled endogenous AE4 expression and was predominantly restricted to type B intercalated cells. Hence this promoter could prove useful to target type B intercalated cells by genetic approaches.
AB - AE4 is an anion exchanger almost exclusively expressed in the collecting ducts of the kidney. This very restricted expression prompted us to analyze its transcription in more detail. 5' RACE yielded alternative transcriptional start sites that are predicted to code for N-terminal protein variants. Comparison of the 5' genomic sequence between species identified a transcriptionally active region with three conserved spans. In transgenic mice beta-galactosidase expression driven by this fragment resembled endogenous AE4 expression and was predominantly restricted to type B intercalated cells. Hence this promoter could prove useful to target type B intercalated cells by genetic approaches.
M3 - SCORING: Zeitschriftenaufsatz
JO - HISTOCHEM CELL BIOL
JF - HISTOCHEM CELL BIOL
SN - 0948-6143
ER -