The HIV-1 regulatory proteins Tat and Rev are frequently targeted by cytotoxic T lymphocytes derived from HIV-1-infected individuals
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The HIV-1 regulatory proteins Tat and Rev are frequently targeted by cytotoxic T lymphocytes derived from HIV-1-infected individuals. / Addo, M M; Altfeld, M; Rosenberg, Eric S.; Eldridge, Robert L; Philips, Mary N.; Habeeb, Kathleen; Khatri, Ashok; Brander, Christian; Robbins, Gregory K.; Mazzara, Gail P.; Goulder, Phillip J; Walker, Bruce D.; HIV Controller Study Collaboration.
in: P NATL ACAD SCI USA, Jahrgang 98, Nr. 4, 13.02.2001, S. 1781-6.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - The HIV-1 regulatory proteins Tat and Rev are frequently targeted by cytotoxic T lymphocytes derived from HIV-1-infected individuals
AU - Addo, M M
AU - Altfeld, M
AU - Rosenberg, Eric S.
AU - Eldridge, Robert L
AU - Philips, Mary N.
AU - Habeeb, Kathleen
AU - Khatri, Ashok
AU - Brander, Christian
AU - Robbins, Gregory K.
AU - Mazzara, Gail P.
AU - Goulder, Phillip J
AU - Walker, Bruce D.
AU - HIV Controller Study Collaboration
PY - 2001/2/13
Y1 - 2001/2/13
N2 - The HIV-1 regulatory proteins Rev and Tat are expressed early in the virus life cycle and thus may be important targets for the immune control of HIV-1-infection and for effective vaccines. However, the extent to which these proteins are targeted in natural HIV-1 infection as well as precise epitopes targeted by human cytotoxic T lymphocytes (CTL) remain to be defined. In the present study, 57 HIV-1-infected individuals were screened for responses against Tat and Rev by using overlapping peptides spanning the entire Tat and Rev proteins. CD8+ T cell responses against Tat and Rev were found in up to 19 and 37% of HIV-1-infected individuals, respectively, indicating that these regulatory proteins are important targets for HIV-1-specific CTL. Despite the small size of these proteins, multiple CTL epitopes were identified in each. These data indicate that Tat and Rev are frequently targeted by CTL in natural HIV-1 infection and may be important targets for HIV vaccines.
AB - The HIV-1 regulatory proteins Rev and Tat are expressed early in the virus life cycle and thus may be important targets for the immune control of HIV-1-infection and for effective vaccines. However, the extent to which these proteins are targeted in natural HIV-1 infection as well as precise epitopes targeted by human cytotoxic T lymphocytes (CTL) remain to be defined. In the present study, 57 HIV-1-infected individuals were screened for responses against Tat and Rev by using overlapping peptides spanning the entire Tat and Rev proteins. CD8+ T cell responses against Tat and Rev were found in up to 19 and 37% of HIV-1-infected individuals, respectively, indicating that these regulatory proteins are important targets for HIV-1-specific CTL. Despite the small size of these proteins, multiple CTL epitopes were identified in each. These data indicate that Tat and Rev are frequently targeted by CTL in natural HIV-1 infection and may be important targets for HIV vaccines.
KW - CD8-Positive T-Lymphocytes/immunology
KW - Epitopes, T-Lymphocyte/immunology
KW - Gene Products, rev/immunology
KW - Gene Products, tat/immunology
KW - HIV Infections/blood
KW - HIV-1/immunology
KW - Humans
KW - Longitudinal Studies
KW - Peptides/immunology
KW - T-Lymphocytes, Cytotoxic/immunology
KW - rev Gene Products, Human Immunodeficiency Virus
KW - tat Gene Products, Human Immunodeficiency Virus
U2 - 10.1073/pnas.98.4.1781
DO - 10.1073/pnas.98.4.1781
M3 - SCORING: Journal article
C2 - 11172028
VL - 98
SP - 1781
EP - 1786
JO - P NATL ACAD SCI USA
JF - P NATL ACAD SCI USA
SN - 0027-8424
IS - 4
ER -