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Subtype-specific expression and genetic alterations of the chemokinereceptor gene CXCR4 in medulloblastomas

Standard

Subtype-specific expression and genetic alterations of the chemokinereceptor gene CXCR4 in medulloblastomas. / Schüller, Ulrich; Koch, Arend; Hartmann, Wolfgang; Garrè, Maria Luisa; Goodyer, Cynthia G; Cama, Armando; Sörensen, Niels; Wiestler, Otmar D; Pietsch, Torsten.

in: INT J CANCER, Jahrgang 117, Nr. 1, 20.10.2005, S. 82-9.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Schüller, U, Koch, A, Hartmann, W, Garrè, ML, Goodyer, CG, Cama, A, Sörensen, N, Wiestler, OD & Pietsch, T 2005, 'Subtype-specific expression and genetic alterations of the chemokinereceptor gene CXCR4 in medulloblastomas', INT J CANCER, Jg. 117, Nr. 1, S. 82-9. https://doi.org/10.1002/ijc.21116

APA

Schüller, U., Koch, A., Hartmann, W., Garrè, M. L., Goodyer, C. G., Cama, A., Sörensen, N., Wiestler, O. D., & Pietsch, T. (2005). Subtype-specific expression and genetic alterations of the chemokinereceptor gene CXCR4 in medulloblastomas. INT J CANCER, 117(1), 82-9. https://doi.org/10.1002/ijc.21116

Vancouver

Schüller U, Koch A, Hartmann W, Garrè ML, Goodyer CG, Cama A et al. Subtype-specific expression and genetic alterations of the chemokinereceptor gene CXCR4 in medulloblastomas. INT J CANCER. 2005 Okt 20;117(1):82-9. https://doi.org/10.1002/ijc.21116

Bibtex

@article{06a864c7ed1f4367baad9cb51ab3e23b,
title = "Subtype-specific expression and genetic alterations of the chemokinereceptor gene CXCR4 in medulloblastomas",
abstract = "Recent findings indicate that the chemokine receptor Cxcr4 is essential for normal development of the cerebellar cortex. As medulloblastomas (MBs), the most common malignant brain tumors of childhood, are believed to arise from neuronal cerebellar precursors, we asked whether there is a potential role for Cxcr4 in the pathogenesis of MB. RT-PCR and immunohistochemistry revealed expression of Cxcr4 in different variants of MBs. Whereas 18/20 classic MBs showed very low levels of CXCR4 mRNA, high amounts were expressed in 17/18 desmoplastic and 6/7 extensively nodular MBs. In addition, a significant correlation of high CXCR4 mRNA levels and presence of the neurotrophin receptor p75NTR or expression of ATOH1 and GLI1 suggests that CXCR4 is a reliable marker for tumors derived from the cerebellar external granular layer. Because Cxcr4 is important for migration and cell cycle control of granular precursors, we screened for mutations in the coding region by SSCP and gene sequencing. In a series of 90 MBs and 8 MB cell lines, we found one germline and one somatic mutation resulting in amino acid substitutions in the first (Ile53Leu) and second (Asp97Asn) transmembrane regions, respectively. These data suggest that Cxcr4 may be involved in the pathogenesis of MBs.",
keywords = "Adolescent, Adult, Amino Acid Substitution, Basic Helix-Loop-Helix Transcription Factors, Cerebellar Neoplasms, Child, Child, Preschool, DNA, DNA Mutational Analysis, DNA-Binding Proteins, Gene Expression Regulation, Neoplastic, Humans, Infant, Medulloblastoma, Middle Aged, Mutation, Nerve Tissue Proteins, Oncogene Proteins, Polymorphism, Single-Stranded Conformational, RNA, RNA, Messenger, Receptor, Nerve Growth Factor, Receptors, CXCR4, Receptors, Nerve Growth Factor, Reverse Transcriptase Polymerase Chain Reaction, Trans-Activators, Transcription Factors, Tumor Cells, Cultured, Zinc Finger Protein GLI1, Comparative Study, Journal Article, Multicenter Study, Research Support, Non-U.S. Gov't",
author = "Ulrich Sch{\"u}ller and Arend Koch and Wolfgang Hartmann and Garr{\`e}, {Maria Luisa} and Goodyer, {Cynthia G} and Armando Cama and Niels S{\"o}rensen and Wiestler, {Otmar D} and Torsten Pietsch",
note = "Copyright (c) 2005 Wiley-Liss, Inc.",
year = "2005",
month = oct,
day = "20",
doi = "10.1002/ijc.21116",
language = "English",
volume = "117",
pages = "82--9",
journal = "INT J CANCER",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "1",

}

RIS

TY - JOUR

T1 - Subtype-specific expression and genetic alterations of the chemokinereceptor gene CXCR4 in medulloblastomas

AU - Schüller, Ulrich

AU - Koch, Arend

AU - Hartmann, Wolfgang

AU - Garrè, Maria Luisa

AU - Goodyer, Cynthia G

AU - Cama, Armando

AU - Sörensen, Niels

AU - Wiestler, Otmar D

AU - Pietsch, Torsten

N1 - Copyright (c) 2005 Wiley-Liss, Inc.

PY - 2005/10/20

Y1 - 2005/10/20

N2 - Recent findings indicate that the chemokine receptor Cxcr4 is essential for normal development of the cerebellar cortex. As medulloblastomas (MBs), the most common malignant brain tumors of childhood, are believed to arise from neuronal cerebellar precursors, we asked whether there is a potential role for Cxcr4 in the pathogenesis of MB. RT-PCR and immunohistochemistry revealed expression of Cxcr4 in different variants of MBs. Whereas 18/20 classic MBs showed very low levels of CXCR4 mRNA, high amounts were expressed in 17/18 desmoplastic and 6/7 extensively nodular MBs. In addition, a significant correlation of high CXCR4 mRNA levels and presence of the neurotrophin receptor p75NTR or expression of ATOH1 and GLI1 suggests that CXCR4 is a reliable marker for tumors derived from the cerebellar external granular layer. Because Cxcr4 is important for migration and cell cycle control of granular precursors, we screened for mutations in the coding region by SSCP and gene sequencing. In a series of 90 MBs and 8 MB cell lines, we found one germline and one somatic mutation resulting in amino acid substitutions in the first (Ile53Leu) and second (Asp97Asn) transmembrane regions, respectively. These data suggest that Cxcr4 may be involved in the pathogenesis of MBs.

AB - Recent findings indicate that the chemokine receptor Cxcr4 is essential for normal development of the cerebellar cortex. As medulloblastomas (MBs), the most common malignant brain tumors of childhood, are believed to arise from neuronal cerebellar precursors, we asked whether there is a potential role for Cxcr4 in the pathogenesis of MB. RT-PCR and immunohistochemistry revealed expression of Cxcr4 in different variants of MBs. Whereas 18/20 classic MBs showed very low levels of CXCR4 mRNA, high amounts were expressed in 17/18 desmoplastic and 6/7 extensively nodular MBs. In addition, a significant correlation of high CXCR4 mRNA levels and presence of the neurotrophin receptor p75NTR or expression of ATOH1 and GLI1 suggests that CXCR4 is a reliable marker for tumors derived from the cerebellar external granular layer. Because Cxcr4 is important for migration and cell cycle control of granular precursors, we screened for mutations in the coding region by SSCP and gene sequencing. In a series of 90 MBs and 8 MB cell lines, we found one germline and one somatic mutation resulting in amino acid substitutions in the first (Ile53Leu) and second (Asp97Asn) transmembrane regions, respectively. These data suggest that Cxcr4 may be involved in the pathogenesis of MBs.

KW - Adolescent

KW - Adult

KW - Amino Acid Substitution

KW - Basic Helix-Loop-Helix Transcription Factors

KW - Cerebellar Neoplasms

KW - Child

KW - Child, Preschool

KW - DNA

KW - DNA Mutational Analysis

KW - DNA-Binding Proteins

KW - Gene Expression Regulation, Neoplastic

KW - Humans

KW - Infant

KW - Medulloblastoma

KW - Middle Aged

KW - Mutation

KW - Nerve Tissue Proteins

KW - Oncogene Proteins

KW - Polymorphism, Single-Stranded Conformational

KW - RNA

KW - RNA, Messenger

KW - Receptor, Nerve Growth Factor

KW - Receptors, CXCR4

KW - Receptors, Nerve Growth Factor

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Trans-Activators

KW - Transcription Factors

KW - Tumor Cells, Cultured

KW - Zinc Finger Protein GLI1

KW - Comparative Study

KW - Journal Article

KW - Multicenter Study

KW - Research Support, Non-U.S. Gov't

U2 - 10.1002/ijc.21116

DO - 10.1002/ijc.21116

M3 - SCORING: Journal article

C2 - 15880586

VL - 117

SP - 82

EP - 89

JO - INT J CANCER

JF - INT J CANCER

SN - 0020-7136

IS - 1

ER -