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Stable Frequencies of HLA-C*03:04/Peptide-Binding KIR2DL2/3+ Natural Killer Cells Following Vaccination

Standard

Stable Frequencies of HLA-C*03:04/Peptide-Binding KIR2DL2/3+ Natural Killer Cells Following Vaccination. / Ziegler, Maja Christiane; Grañana, Ferran Borràs; Garcia-Beltran, Wilfredo F; Schulze Zur Wiesch, Julian; Hoffmann, Christian; Rechtien, Anne; Lunemann, Sebastian; Altfeld, Marcus.

in: FRONT IMMUNOL, Jahrgang 9, 2018, S. 2361.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Ziegler, MC, Grañana, FB, Garcia-Beltran, WF, Schulze Zur Wiesch, J, Hoffmann, C, Rechtien, A, Lunemann, S & Altfeld, M 2018, 'Stable Frequencies of HLA-C*03:04/Peptide-Binding KIR2DL2/3+ Natural Killer Cells Following Vaccination', FRONT IMMUNOL, Jg. 9, S. 2361. https://doi.org/10.3389/fimmu.2018.02361

APA

Ziegler, M. C., Grañana, F. B., Garcia-Beltran, W. F., Schulze Zur Wiesch, J., Hoffmann, C., Rechtien, A., Lunemann, S., & Altfeld, M. (2018). Stable Frequencies of HLA-C*03:04/Peptide-Binding KIR2DL2/3+ Natural Killer Cells Following Vaccination. FRONT IMMUNOL, 9, 2361. https://doi.org/10.3389/fimmu.2018.02361

Vancouver

Ziegler MC, Grañana FB, Garcia-Beltran WF, Schulze Zur Wiesch J, Hoffmann C, Rechtien A et al. Stable Frequencies of HLA-C*03:04/Peptide-Binding KIR2DL2/3+ Natural Killer Cells Following Vaccination. FRONT IMMUNOL. 2018;9:2361. https://doi.org/10.3389/fimmu.2018.02361

Bibtex

@article{a00ce0d80123430fbb9dcfc9e5164183,
title = "Stable Frequencies of HLA-C*03:04/Peptide-Binding KIR2DL2/3+ Natural Killer Cells Following Vaccination",
abstract = "Inhibitory KIRs play a central role in regulating NK cell activity. KIR2DL2/3 bind to HLA-C molecules, but the modulation of these interactions by viral infections and presentation of viral epitopes is not well-understood. We investigated whether the frequencies of KIR2DL2/3+ NK cells recognizing HLA-C*03:04/viral peptide complexes were impacted by YFV vaccination or HIV-1 and HCV infection. Ex vivo HLA class I tetramer staining of primary human NK cells derived from YFV-vaccinated individuals, or HIV-1- or HCV-infected individuals revealed that the YFV/HLA-C*03:04-NS2A4-13-tetramer bound to a larger proportion of KIR2DL2/3+ NK cells compared to HIV-1/HLA-C*03:04-Gag296-304- or HCV/HLA-C*03:04-Core136-144-tetramers. The YFV/HLA-C*03:04-NS2A4-13-tetramer also exhibited a stronger avidity to KIR2DL2/3 compared to the other tested tetramers. The proportional frequencies of KIR2DL2/3+ NK cells binding to the three tested HLA-C*03:04 tetramers were identical between YFV-vaccinated individuals or HIV-1- or HCV-infected individuals, and remained stable following YFV vaccination. These data demonstrate consistent hierarchies in the frequency of primary KIR2DL2/3+ NK cells binding HLA-C*03:04/peptide complexes that were determined by the HLA-C-presented peptide and not modulated by the underlying viral infection or vaccination.",
keywords = "Flow Cytometry, HLA-C Antigens/chemistry, Humans, Killer Cells, Natural/immunology, Leukocytes, Mononuclear/immunology, Multiprotein Complexes, Peptides/chemistry, Protein Binding, Receptors, KIR2DL2/metabolism, Receptors, KIR2DL3/metabolism, Vaccination, Vaccines/immunology",
author = "Ziegler, {Maja Christiane} and Gra{\~n}ana, {Ferran Borr{\`a}s} and Garcia-Beltran, {Wilfredo F} and {Schulze Zur Wiesch}, Julian and Christian Hoffmann and Anne Rechtien and Sebastian Lunemann and Marcus Altfeld",
year = "2018",
doi = "10.3389/fimmu.2018.02361",
language = "English",
volume = "9",
pages = "2361",
journal = "FRONT IMMUNOL",
issn = "1664-3224",
publisher = "Lausanne : Frontiers Research Foundation",

}

RIS

TY - JOUR

T1 - Stable Frequencies of HLA-C*03:04/Peptide-Binding KIR2DL2/3+ Natural Killer Cells Following Vaccination

AU - Ziegler, Maja Christiane

AU - Grañana, Ferran Borràs

AU - Garcia-Beltran, Wilfredo F

AU - Schulze Zur Wiesch, Julian

AU - Hoffmann, Christian

AU - Rechtien, Anne

AU - Lunemann, Sebastian

AU - Altfeld, Marcus

PY - 2018

Y1 - 2018

N2 - Inhibitory KIRs play a central role in regulating NK cell activity. KIR2DL2/3 bind to HLA-C molecules, but the modulation of these interactions by viral infections and presentation of viral epitopes is not well-understood. We investigated whether the frequencies of KIR2DL2/3+ NK cells recognizing HLA-C*03:04/viral peptide complexes were impacted by YFV vaccination or HIV-1 and HCV infection. Ex vivo HLA class I tetramer staining of primary human NK cells derived from YFV-vaccinated individuals, or HIV-1- or HCV-infected individuals revealed that the YFV/HLA-C*03:04-NS2A4-13-tetramer bound to a larger proportion of KIR2DL2/3+ NK cells compared to HIV-1/HLA-C*03:04-Gag296-304- or HCV/HLA-C*03:04-Core136-144-tetramers. The YFV/HLA-C*03:04-NS2A4-13-tetramer also exhibited a stronger avidity to KIR2DL2/3 compared to the other tested tetramers. The proportional frequencies of KIR2DL2/3+ NK cells binding to the three tested HLA-C*03:04 tetramers were identical between YFV-vaccinated individuals or HIV-1- or HCV-infected individuals, and remained stable following YFV vaccination. These data demonstrate consistent hierarchies in the frequency of primary KIR2DL2/3+ NK cells binding HLA-C*03:04/peptide complexes that were determined by the HLA-C-presented peptide and not modulated by the underlying viral infection or vaccination.

AB - Inhibitory KIRs play a central role in regulating NK cell activity. KIR2DL2/3 bind to HLA-C molecules, but the modulation of these interactions by viral infections and presentation of viral epitopes is not well-understood. We investigated whether the frequencies of KIR2DL2/3+ NK cells recognizing HLA-C*03:04/viral peptide complexes were impacted by YFV vaccination or HIV-1 and HCV infection. Ex vivo HLA class I tetramer staining of primary human NK cells derived from YFV-vaccinated individuals, or HIV-1- or HCV-infected individuals revealed that the YFV/HLA-C*03:04-NS2A4-13-tetramer bound to a larger proportion of KIR2DL2/3+ NK cells compared to HIV-1/HLA-C*03:04-Gag296-304- or HCV/HLA-C*03:04-Core136-144-tetramers. The YFV/HLA-C*03:04-NS2A4-13-tetramer also exhibited a stronger avidity to KIR2DL2/3 compared to the other tested tetramers. The proportional frequencies of KIR2DL2/3+ NK cells binding to the three tested HLA-C*03:04 tetramers were identical between YFV-vaccinated individuals or HIV-1- or HCV-infected individuals, and remained stable following YFV vaccination. These data demonstrate consistent hierarchies in the frequency of primary KIR2DL2/3+ NK cells binding HLA-C*03:04/peptide complexes that were determined by the HLA-C-presented peptide and not modulated by the underlying viral infection or vaccination.

KW - Flow Cytometry

KW - HLA-C Antigens/chemistry

KW - Humans

KW - Killer Cells, Natural/immunology

KW - Leukocytes, Mononuclear/immunology

KW - Multiprotein Complexes

KW - Peptides/chemistry

KW - Protein Binding

KW - Receptors, KIR2DL2/metabolism

KW - Receptors, KIR2DL3/metabolism

KW - Vaccination

KW - Vaccines/immunology

U2 - 10.3389/fimmu.2018.02361

DO - 10.3389/fimmu.2018.02361

M3 - SCORING: Journal article

C2 - 30386333

VL - 9

SP - 2361

JO - FRONT IMMUNOL

JF - FRONT IMMUNOL

SN - 1664-3224

ER -