Signature of circulating microRNAs in osteoarthritis
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Signature of circulating microRNAs in osteoarthritis. / Beyer, Christian; Zampetaki, Anna; Lin, Neng-Yu; Kleyer, Arnd; Perricone, Carlo; Iagnocco, Annamaria; Distler, Alfiya; Langley, Sarah R; Gelse, Kolja; Sesselmann, Stefan; Lorenzini, Rolando; Niemeier, Andreas; Swoboda, Bernd; Distler, Jörg H W; Santer, Peter; Egger, Georg; Willeit, Johann; Mayr, Manuel; Schett, Georg; Kiechl, Stefan.
in: ANN RHEUM DIS, Jahrgang 74, Nr. 1, 01.03.2014, S. e18.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - Signature of circulating microRNAs in osteoarthritis
AU - Beyer, Christian
AU - Zampetaki, Anna
AU - Lin, Neng-Yu
AU - Kleyer, Arnd
AU - Perricone, Carlo
AU - Iagnocco, Annamaria
AU - Distler, Alfiya
AU - Langley, Sarah R
AU - Gelse, Kolja
AU - Sesselmann, Stefan
AU - Lorenzini, Rolando
AU - Niemeier, Andreas
AU - Swoboda, Bernd
AU - Distler, Jörg H W
AU - Santer, Peter
AU - Egger, Georg
AU - Willeit, Johann
AU - Mayr, Manuel
AU - Schett, Georg
AU - Kiechl, Stefan
PY - 2014/3/1
Y1 - 2014/3/1
N2 - BACKGROUND: Osteoarthritis is the most common form of arthritis and a major socioeconomic burden. Our study is the first to explore the association between serum microRNA levels and the development of severe osteoarthritis of the knee and hip joint in the general population.METHODS: We followed 816 Caucasian individuals from 1995 to 2010 and assessed joint arthroplasty as a definitive outcome of severe osteoarthritis of the knee and hip. After a microarray screen, we validated 12 microRNAs by real-time PCR in the entire cohort at baseline.RESULTS: In Cox regression analysis, three microRNAs were associated with severe knee and hip osteoarthritis. let-7e was a negative predictor for total joint arthroplasty with an adjusted HR of 0.75 (95% CI 0.58 to 0.96; p=0.021) when normalised to U6, and 0.76 (95% CI 0.6 to 0.97; p=0.026) after normalisation to the Ct average. miRNA-454 was inversely correlated with severe knee or hip osteoarthritis with an adjusted HR of 0.77 (95% CI 0.61 to 0.97; p=0.028) when normalised to U6. This correlation was lost when data were normalised to Ct average (p=0.118). Finally, miRNA-885-5p showed a trend towards a positive relationship with arthroplasty when normalised to U6 (HR 1.24; 95% CI 0.95 to 1.62; p=0.107) or to Ct average (HR 1.30; 95% CI 0.99 to 1.70; p=0.056).CONCLUSIONS: Our study is the first to identify differentially expressed circulating microRNAs in osteoarthritis patients necessitating arthroplasty in a large, population-based cohort. Among these microRNAs, let-7e emerged as potential predictor for severe knee or hip osteoarthritis.
AB - BACKGROUND: Osteoarthritis is the most common form of arthritis and a major socioeconomic burden. Our study is the first to explore the association between serum microRNA levels and the development of severe osteoarthritis of the knee and hip joint in the general population.METHODS: We followed 816 Caucasian individuals from 1995 to 2010 and assessed joint arthroplasty as a definitive outcome of severe osteoarthritis of the knee and hip. After a microarray screen, we validated 12 microRNAs by real-time PCR in the entire cohort at baseline.RESULTS: In Cox regression analysis, three microRNAs were associated with severe knee and hip osteoarthritis. let-7e was a negative predictor for total joint arthroplasty with an adjusted HR of 0.75 (95% CI 0.58 to 0.96; p=0.021) when normalised to U6, and 0.76 (95% CI 0.6 to 0.97; p=0.026) after normalisation to the Ct average. miRNA-454 was inversely correlated with severe knee or hip osteoarthritis with an adjusted HR of 0.77 (95% CI 0.61 to 0.97; p=0.028) when normalised to U6. This correlation was lost when data were normalised to Ct average (p=0.118). Finally, miRNA-885-5p showed a trend towards a positive relationship with arthroplasty when normalised to U6 (HR 1.24; 95% CI 0.95 to 1.62; p=0.107) or to Ct average (HR 1.30; 95% CI 0.99 to 1.70; p=0.056).CONCLUSIONS: Our study is the first to identify differentially expressed circulating microRNAs in osteoarthritis patients necessitating arthroplasty in a large, population-based cohort. Among these microRNAs, let-7e emerged as potential predictor for severe knee or hip osteoarthritis.
U2 - 10.1136/annrheumdis-2013-204698
DO - 10.1136/annrheumdis-2013-204698
M3 - SCORING: Journal article
C2 - 24515954
VL - 74
SP - e18
JO - ANN RHEUM DIS
JF - ANN RHEUM DIS
SN - 0003-4967
IS - 1
ER -