Role of delta-like-4/Notch in the formation and wiring of the lymphatic network in zebrafish
Abstract
OBJECTIVE: To study whether Notch signaling, which regulates cell fate decisions and vessel morphogenesis, controls lymphatic development.
METHODS AND RESULTS: In zebrafish embryos, sprouts from the axial vein have lymphangiogenic potential because they give rise to the first lymphatics. Knockdown of delta-like-4 (Dll4) or its receptors Notch-1b or Notch-6 in zebrafish impaired lymphangiogenesis. Dll4/Notch silencing reduced the number of sprouts producing the string of parchordal lymphangioblasts; instead, sprouts connecting to the intersomitic vessels were formed. At a later phase, Notch silencing impaired navigation of lymphatic intersomitic vessels along their arterial templates.
CONCLUSIONS: These studies imply critical roles for Notch signaling in the formation and wiring of the lymphatic network.
Bibliografische Daten
Originalsprache | Englisch |
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ISSN | 1079-5642 |
DOIs | |
Status | Veröffentlicht - 09.2010 |
Extern publiziert | Ja |
PubMed | 20466977 |
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