Repair of oronasal fistulas with human amniotic membrane in minipigs
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Repair of oronasal fistulas with human amniotic membrane in minipigs. / Kesting, Marco Rainer; Loeffelbein, Denys John; Classen, Mariella; Slotta-Huspenina, Julia; Hasler, Rafael Johannes; Jacobsen, Frank; Kreutzer, Kilian; Al-Benna, Sammy; Wolff, Klaus-Dietrich; Steinstraesser, Lars.
in: BRIT J ORAL MAX SURG, Jahrgang 48, Nr. 2, 03.2010, S. 131–135.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Repair of oronasal fistulas with human amniotic membrane in minipigs
AU - Kesting, Marco Rainer
AU - Loeffelbein, Denys John
AU - Classen, Mariella
AU - Slotta-Huspenina, Julia
AU - Hasler, Rafael Johannes
AU - Jacobsen, Frank
AU - Kreutzer, Kilian
AU - Al-Benna, Sammy
AU - Wolff, Klaus-Dietrich
AU - Steinstraesser, Lars
PY - 2010/3
Y1 - 2010/3
N2 - We evaluated the use of multilayer human amniotic membrane (HAM) as a grafting material for the repair of mid-palate oronasal fistulas in seven Berlin minipigs. After two weeks, three animals had the fistulas repaired with multilayered HAM grafts, three had them repaired with a collagen-based dermal substitute (INTEGRA((R)), Integra Life Sciences, Plainsboro, NJ, USA), and one fistula was left untreated to serve as a control. Grafts were interposed between the oral and nasal mucosa, traversing the fistulas. After healing for 40 days, the pigs were killed for clinical, histological, and immunohistochemical examination. Two of the three fistulas closed with HAM were successful, the diameter of the third was reduced in size, and there was no change in the diameter of the fistula in the control. This study shows successful closure of oronasal fistulas in minipigs using interposed grafts of cryopreserved HAM, and offers promise as a simple and effective technique for tension-free closure of such fistulas.
AB - We evaluated the use of multilayer human amniotic membrane (HAM) as a grafting material for the repair of mid-palate oronasal fistulas in seven Berlin minipigs. After two weeks, three animals had the fistulas repaired with multilayered HAM grafts, three had them repaired with a collagen-based dermal substitute (INTEGRA((R)), Integra Life Sciences, Plainsboro, NJ, USA), and one fistula was left untreated to serve as a control. Grafts were interposed between the oral and nasal mucosa, traversing the fistulas. After healing for 40 days, the pigs were killed for clinical, histological, and immunohistochemical examination. Two of the three fistulas closed with HAM were successful, the diameter of the third was reduced in size, and there was no change in the diameter of the fistula in the control. This study shows successful closure of oronasal fistulas in minipigs using interposed grafts of cryopreserved HAM, and offers promise as a simple and effective technique for tension-free closure of such fistulas.
KW - Amnion
KW - Animals
KW - Chondroitin Sulfates
KW - Collagen
KW - Humans
KW - Male
KW - Nose Diseases
KW - Oral Fistula
KW - Respiratory Tract Fistula
KW - Skin, Artificial
KW - Swine
KW - Swine, Miniature
U2 - 10.1016/j.bjoms.2009.04.025
DO - 10.1016/j.bjoms.2009.04.025
M3 - SCORING: Journal article
C2 - 19477056
VL - 48
SP - 131
EP - 135
JO - BRIT J ORAL MAX SURG
JF - BRIT J ORAL MAX SURG
SN - 0266-4356
IS - 2
ER -