Regulation of rat mesangial cell growth by diadenosine phosphates
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Regulation of rat mesangial cell growth by diadenosine phosphates. / Heidenreich, Stefan; Tepel, M; Schlüter, H; Harrach, B; Zidek, W; Heidenreich, Stefan.
in: J CLIN INVEST, Jahrgang 95, Nr. 6, 06.1995, S. 2862-7.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Regulation of rat mesangial cell growth by diadenosine phosphates
AU - Heidenreich, Stefan
AU - Tepel, M
AU - Schlüter, H
AU - Harrach, B
AU - Zidek, W
AU - Heidenreich, Stefan
PY - 1995/6
Y1 - 1995/6
N2 - The newly recognized human endogenous vasoconstrictive dinucleotides, diadenosine pentaphosphate (AP5A) and diadenosine hexaphosphate (AP6A), were tested for growth stimulatory effects in rat mesangial cells (MC). Both AP5A and AP6A stimulated growth in micromolar concentrations. The growth stimulatory effect exceeded that of ATP, alpha,beta-methylene ATP, adenosine 5'-O-(3-thio)triphosphate and UTP. Both diadenosine phosphates potentiated the growth response to platelet-derived growth factor, but not to insulin-like growth factor-1. To further elucidate the site of action in the cell cycle, RNA and protein synthesis were assessed. AP5 and AP6A stimulated protein synthesis, but not RNA formation. Furthermore, both agents increased cytosolic free Ca2+ concentration. It is concluded that AP5A and AP6A may play a regulatory role in MC growth as progression factors and possibly modify MC proliferation in glomerular disease.
AB - The newly recognized human endogenous vasoconstrictive dinucleotides, diadenosine pentaphosphate (AP5A) and diadenosine hexaphosphate (AP6A), were tested for growth stimulatory effects in rat mesangial cells (MC). Both AP5A and AP6A stimulated growth in micromolar concentrations. The growth stimulatory effect exceeded that of ATP, alpha,beta-methylene ATP, adenosine 5'-O-(3-thio)triphosphate and UTP. Both diadenosine phosphates potentiated the growth response to platelet-derived growth factor, but not to insulin-like growth factor-1. To further elucidate the site of action in the cell cycle, RNA and protein synthesis were assessed. AP5 and AP6A stimulated protein synthesis, but not RNA formation. Furthermore, both agents increased cytosolic free Ca2+ concentration. It is concluded that AP5A and AP6A may play a regulatory role in MC growth as progression factors and possibly modify MC proliferation in glomerular disease.
KW - Adenosine
KW - Adenosine Triphosphate
KW - Animals
KW - Calcium
KW - Cell Division
KW - Cells, Cultured
KW - DNA
KW - Dinucleoside Phosphates
KW - Drug Synergism
KW - Glomerular Mesangium
KW - In Vitro Techniques
KW - Male
KW - Platelet-Derived Growth Factor
KW - Rats
KW - Rats, Inbred WKY
KW - Uridine Triphosphate
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1172/JCI117992
DO - 10.1172/JCI117992
M3 - SCORING: Journal article
C2 - 7769127
VL - 95
SP - 2862
EP - 2867
JO - J CLIN INVEST
JF - J CLIN INVEST
SN - 0021-9738
IS - 6
ER -