Redifferentiation of chondrocytes and cartilage formation under intermittent hydrostatic pressure.
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Redifferentiation of chondrocytes and cartilage formation under intermittent hydrostatic pressure. / Heyland, Jan; Wiegandt, Katharina; Goepfert, Christiane; Nagel-Heyer, Stefanie; Ilinich, Eduard; Schumacher, Udo; Pörtner, Ralf.
in: BIOTECHNOL LETT, Jahrgang 28, Nr. 20, 20, 2006, S. 1641-1648.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Redifferentiation of chondrocytes and cartilage formation under intermittent hydrostatic pressure.
AU - Heyland, Jan
AU - Wiegandt, Katharina
AU - Goepfert, Christiane
AU - Nagel-Heyer, Stefanie
AU - Ilinich, Eduard
AU - Schumacher, Udo
AU - Pörtner, Ralf
PY - 2006
Y1 - 2006
N2 - Since articular cartilage is subjected to varying loads in vivo and undergoes cyclic hydrostatic pressure during periods of loading, it is hypothesized that mimicking these in vivo conditions can enhance synthesis of important matrix components during cultivation in vitro. Thus, the influence of intermittent loading during redifferentiation of chondrocytes in alginate beads, and during cartilage formation was investigated. A statistically significant increased synthesis of glycosaminoglycan and collagen type II during redifferentiation of chondrocytes embedded in alginate beads, as well as an increase in glycosaminoglycan content of tissue-engineered cartilage, was found compared to control without load. Immunohistological staining indicated qualitatively a high expression of collagen type II for both cases.
AB - Since articular cartilage is subjected to varying loads in vivo and undergoes cyclic hydrostatic pressure during periods of loading, it is hypothesized that mimicking these in vivo conditions can enhance synthesis of important matrix components during cultivation in vitro. Thus, the influence of intermittent loading during redifferentiation of chondrocytes in alginate beads, and during cartilage formation was investigated. A statistically significant increased synthesis of glycosaminoglycan and collagen type II during redifferentiation of chondrocytes embedded in alginate beads, as well as an increase in glycosaminoglycan content of tissue-engineered cartilage, was found compared to control without load. Immunohistological staining indicated qualitatively a high expression of collagen type II for both cases.
M3 - SCORING: Zeitschriftenaufsatz
VL - 28
SP - 1641
EP - 1648
JO - BIOTECHNOL LETT
JF - BIOTECHNOL LETT
SN - 0141-5492
IS - 20
M1 - 20
ER -