Reappearance of effector T cells is associated with recovery from COVID-19
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Reappearance of effector T cells is associated with recovery from COVID-19. / Odak, Ivan; Barros-Martins, Joana; Bošnjak, Berislav; Stahl, Klaus; David, Sascha; Wiesner, Olaf; Busch, Markus; Hoeper, Marius M; Pink, Isabell; Welte, Tobias; Cornberg, Markus; Stoll, Matthias; Goudeva, Lilia; Blasczyk, Rainer; Ganser, Arnold; Prinz, Immo; Förster, Reinhold; Koenecke, Christian; Schultze-Florey, Christian R.
in: EBIOMEDICINE, Jahrgang 57, 102885, 07.2020.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - Reappearance of effector T cells is associated with recovery from COVID-19
AU - Odak, Ivan
AU - Barros-Martins, Joana
AU - Bošnjak, Berislav
AU - Stahl, Klaus
AU - David, Sascha
AU - Wiesner, Olaf
AU - Busch, Markus
AU - Hoeper, Marius M
AU - Pink, Isabell
AU - Welte, Tobias
AU - Cornberg, Markus
AU - Stoll, Matthias
AU - Goudeva, Lilia
AU - Blasczyk, Rainer
AU - Ganser, Arnold
AU - Prinz, Immo
AU - Förster, Reinhold
AU - Koenecke, Christian
AU - Schultze-Florey, Christian R
N1 - Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.
PY - 2020/7
Y1 - 2020/7
N2 - BACKGROUND: Elucidating the role of T cell responses in COVID-19 is of utmost importance to understand the clearance of SARS-CoV-2 infection.METHODS: 30 hospitalized COVID-19 patients and 60 age- and gender-matched healthy controls (HC) participated in this study. We used two comprehensive 11-colour flow cytometric panels conforming to Good Laboratory Practice and approved for clinical diagnostics.FINDINGS: Absolute numbers of lymphocyte subsets were differentially decreased in COVID-19 patients according to clinical severity. In severe disease (SD) patients, all lymphocyte subsets were reduced, whilst in mild disease (MD) NK, NKT and γδ T cells were at the level of HC. Additionally, we provide evidence of T cell activation in MD but not SD, when compared to HC. Follow up samples revealed a marked increase in effector T cells and memory subsets in convalescing but not in non-convalescing patients.INTERPRETATION: Our data suggest that activation and expansion of innate and adaptive lymphocytes play a major role in COVID-19. Additionally, recovery is associated with formation of T cell memory as suggested by the missing formation of effector and central memory T cells in SD but not in MD. Understanding T cell-responses in the context of clinical severity might serve as foundation to overcome the lack of effective anti-viral immune response in severely affected COVID-19 patients and can offer prognostic value as biomarker for disease outcome and control.FUNDING: Funded by State of Lower Saxony grant 14-76,103-184CORONA-11/20 and German Research Foundation, Excellence Strategy - EXC2155"RESIST"-Project ID39087428, and DFG-SFB900/3-Project ID158989968, grants SFB900-B3, SFB900-B8.
AB - BACKGROUND: Elucidating the role of T cell responses in COVID-19 is of utmost importance to understand the clearance of SARS-CoV-2 infection.METHODS: 30 hospitalized COVID-19 patients and 60 age- and gender-matched healthy controls (HC) participated in this study. We used two comprehensive 11-colour flow cytometric panels conforming to Good Laboratory Practice and approved for clinical diagnostics.FINDINGS: Absolute numbers of lymphocyte subsets were differentially decreased in COVID-19 patients according to clinical severity. In severe disease (SD) patients, all lymphocyte subsets were reduced, whilst in mild disease (MD) NK, NKT and γδ T cells were at the level of HC. Additionally, we provide evidence of T cell activation in MD but not SD, when compared to HC. Follow up samples revealed a marked increase in effector T cells and memory subsets in convalescing but not in non-convalescing patients.INTERPRETATION: Our data suggest that activation and expansion of innate and adaptive lymphocytes play a major role in COVID-19. Additionally, recovery is associated with formation of T cell memory as suggested by the missing formation of effector and central memory T cells in SD but not in MD. Understanding T cell-responses in the context of clinical severity might serve as foundation to overcome the lack of effective anti-viral immune response in severely affected COVID-19 patients and can offer prognostic value as biomarker for disease outcome and control.FUNDING: Funded by State of Lower Saxony grant 14-76,103-184CORONA-11/20 and German Research Foundation, Excellence Strategy - EXC2155"RESIST"-Project ID39087428, and DFG-SFB900/3-Project ID158989968, grants SFB900-B3, SFB900-B8.
KW - Adult
KW - Aged
KW - Aged, 80 and over
KW - Betacoronavirus/immunology
KW - Biomarkers
KW - CD4-Positive T-Lymphocytes/cytology
KW - CD8-Positive T-Lymphocytes/cytology
KW - COVID-19
KW - Coronavirus Infections/immunology
KW - Female
KW - Humans
KW - Immunologic Memory/immunology
KW - Lymphocyte Activation/immunology
KW - Lymphocyte Count
KW - Male
KW - Middle Aged
KW - Pandemics
KW - Pneumonia, Viral/immunology
KW - Prognosis
KW - SARS-CoV-2
KW - Severity of Illness Index
KW - Young Adult
U2 - 10.1016/j.ebiom.2020.102885
DO - 10.1016/j.ebiom.2020.102885
M3 - SCORING: Journal article
C2 - 32650275
VL - 57
JO - EBIOMEDICINE
JF - EBIOMEDICINE
SN - 2352-3964
M1 - 102885
ER -