Quality control of inclusion bodies in Escherichia coli.
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Quality control of inclusion bodies in Escherichia coli. / Jürgen, Britta; Breitenstein, Antje; Urlacher, Vlada; Büttner, Knut; Lin, Hongying; Hecker, Michael; Schweder, Thomas; Neubauer, Peter.
in: MICROB CELL FACT, Jahrgang 9, 2010, S. 41.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Quality control of inclusion bodies in Escherichia coli.
AU - Jürgen, Britta
AU - Breitenstein, Antje
AU - Urlacher, Vlada
AU - Büttner, Knut
AU - Lin, Hongying
AU - Hecker, Michael
AU - Schweder, Thomas
AU - Neubauer, Peter
PY - 2010
Y1 - 2010
N2 - Bacterial inclusion bodies (IBs) are key intermediates for protein production. Their quality affects the refolding yield and further purification. Recent functional and structural studies have revealed that IBs are not dead-end aggregates but undergo dynamic changes, including aggregation, refunctionalization of the protein and proteolysis. Both, aggregation of the folding intermediates and turnover of IBs are influenced by the cellular situation and a number of well-studied chaperones and proteases are included. IBs mostly contain only minor impurities and are relatively homogenous.
AB - Bacterial inclusion bodies (IBs) are key intermediates for protein production. Their quality affects the refolding yield and further purification. Recent functional and structural studies have revealed that IBs are not dead-end aggregates but undergo dynamic changes, including aggregation, refunctionalization of the protein and proteolysis. Both, aggregation of the folding intermediates and turnover of IBs are influenced by the cellular situation and a number of well-studied chaperones and proteases are included. IBs mostly contain only minor impurities and are relatively homogenous.
U2 - 10.1186/1475-2859-9-41
DO - 10.1186/1475-2859-9-41
M3 - SCORING: Zeitschriftenaufsatz
VL - 9
SP - 41
JO - MICROB CELL FACT
JF - MICROB CELL FACT
SN - 1475-2859
ER -