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Purification and functional characterization of the human beta 2-adrenergic receptor produced in baculovirus-infected insect cells.

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Purification and functional characterization of the human beta 2-adrenergic receptor produced in baculovirus-infected insect cells. / Reiländer, H; Boege, F; Vasudevan, S; Maul, G; Hekman, M; Dees, C; Hampe, Wolfgang; Helmreich, E J; Michel, H.

in: FEBS LETT, Jahrgang 282, Nr. 2, 2, 1991, S. 441-444.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Reiländer, H, Boege, F, Vasudevan, S, Maul, G, Hekman, M, Dees, C, Hampe, W, Helmreich, EJ & Michel, H 1991, 'Purification and functional characterization of the human beta 2-adrenergic receptor produced in baculovirus-infected insect cells.', FEBS LETT, Jg. 282, Nr. 2, 2, S. 441-444. <http://www.ncbi.nlm.nih.gov/pubmed/1645288?dopt=Citation>

APA

Reiländer, H., Boege, F., Vasudevan, S., Maul, G., Hekman, M., Dees, C., Hampe, W., Helmreich, E. J., & Michel, H. (1991). Purification and functional characterization of the human beta 2-adrenergic receptor produced in baculovirus-infected insect cells. FEBS LETT, 282(2), 441-444. [2]. http://www.ncbi.nlm.nih.gov/pubmed/1645288?dopt=Citation

Vancouver

Reiländer H, Boege F, Vasudevan S, Maul G, Hekman M, Dees C et al. Purification and functional characterization of the human beta 2-adrenergic receptor produced in baculovirus-infected insect cells. FEBS LETT. 1991;282(2):441-444. 2.

Bibtex

@article{1d31415c3e07442199c23d300da6578e,
title = "Purification and functional characterization of the human beta 2-adrenergic receptor produced in baculovirus-infected insect cells.",
abstract = "A human cDNA fragment bearing the complete coding region for the beta 2-adrenergic receptor was introduced into the genome of Autographa california nuclear polyhedrosis virus under the control of the polyhedrin promoter. Binding studies using [125I]iodocyanopindolol showed that Sf9 insect cells infected with the recombinant virus expressed approximately 1 x 10(6) beta 2-adrenergic receptors on their cell surface. Photoaffinity labeling of whole cells and membranes revealed a molecular weight of approximately 46,000 for the expressed receptor. The receptor produced in insect cells is glycosylated but the extent and pattern differ from that of the receptor from human tissue. The heterologously expressed receptor was purified by alprenolol affinity chromatography, and was able to activate isolated Gs-protein.",
author = "H Reil{\"a}nder and F Boege and S Vasudevan and G Maul and M Hekman and C Dees and Wolfgang Hampe and Helmreich, {E J} and H Michel",
year = "1991",
language = "Deutsch",
volume = "282",
pages = "441--444",
journal = "FEBS LETT",
issn = "0014-5793",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Purification and functional characterization of the human beta 2-adrenergic receptor produced in baculovirus-infected insect cells.

AU - Reiländer, H

AU - Boege, F

AU - Vasudevan, S

AU - Maul, G

AU - Hekman, M

AU - Dees, C

AU - Hampe, Wolfgang

AU - Helmreich, E J

AU - Michel, H

PY - 1991

Y1 - 1991

N2 - A human cDNA fragment bearing the complete coding region for the beta 2-adrenergic receptor was introduced into the genome of Autographa california nuclear polyhedrosis virus under the control of the polyhedrin promoter. Binding studies using [125I]iodocyanopindolol showed that Sf9 insect cells infected with the recombinant virus expressed approximately 1 x 10(6) beta 2-adrenergic receptors on their cell surface. Photoaffinity labeling of whole cells and membranes revealed a molecular weight of approximately 46,000 for the expressed receptor. The receptor produced in insect cells is glycosylated but the extent and pattern differ from that of the receptor from human tissue. The heterologously expressed receptor was purified by alprenolol affinity chromatography, and was able to activate isolated Gs-protein.

AB - A human cDNA fragment bearing the complete coding region for the beta 2-adrenergic receptor was introduced into the genome of Autographa california nuclear polyhedrosis virus under the control of the polyhedrin promoter. Binding studies using [125I]iodocyanopindolol showed that Sf9 insect cells infected with the recombinant virus expressed approximately 1 x 10(6) beta 2-adrenergic receptors on their cell surface. Photoaffinity labeling of whole cells and membranes revealed a molecular weight of approximately 46,000 for the expressed receptor. The receptor produced in insect cells is glycosylated but the extent and pattern differ from that of the receptor from human tissue. The heterologously expressed receptor was purified by alprenolol affinity chromatography, and was able to activate isolated Gs-protein.

M3 - SCORING: Zeitschriftenaufsatz

VL - 282

SP - 441

EP - 444

JO - FEBS LETT

JF - FEBS LETT

SN - 0014-5793

IS - 2

M1 - 2

ER -