Protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies

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Protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies. / Giannou, Anastasios D; Ohm, Birte; Zazara, Dimitra E; Lücke, Jöran; Zhang, Tao; Sabihi, Morsal; Seeger, Philipp; Oh, Jun; Grotelüschen, Rainer; Busch, Philipp; Mann, Oliver; Hackert, Thilo; Izbicki, Jakob R; Yamada, Yoshito; Huber, Samuel; Jungraithmayr, Wolfgang.

in: STAR protocols, Jahrgang 4, Nr. 4, 15.12.2023, S. 102701.

Publikationen: SCORING: Beitrag in Fachzeitschrift/ZeitungSCORING: ZeitschriftenaufsatzForschungBegutachtung

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@article{d14082aa2ade4190ab2d9f67f2d6a3c8,
title = "Protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies",
abstract = "The transplantation model provides the opportunity to assess the relevance of a molecule of interest for tumor cell extravasation by using a respective genetically modified donor animal. Here, we present a protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies. We describe steps for animal preparation, lung transplantation, and tumor cell injection. We then detail procedures for the direct comparison of tumor cell spreading between the genetically modified left lung and the naive right lung parenchyma. For complete details on the use and execution of this protocol, please refer to Giannou et al. (2023).1.",
author = "Giannou, {Anastasios D} and Birte Ohm and Zazara, {Dimitra E} and J{\"o}ran L{\"u}cke and Tao Zhang and Morsal Sabihi and Philipp Seeger and Jun Oh and Rainer Grotel{\"u}schen and Philipp Busch and Oliver Mann and Thilo Hackert and Izbicki, {Jakob R} and Yoshito Yamada and Samuel Huber and Wolfgang Jungraithmayr",
note = "Copyright {\textcopyright} 2023 The Author(s). Published by Elsevier Inc. All rights reserved.",
year = "2023",
month = dec,
day = "15",
doi = "10.1016/j.xpro.2023.102701",
language = "English",
volume = "4",
pages = "102701",
journal = "STAR protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "4",

}

RIS

TY - JOUR

T1 - Protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies

AU - Giannou, Anastasios D

AU - Ohm, Birte

AU - Zazara, Dimitra E

AU - Lücke, Jöran

AU - Zhang, Tao

AU - Sabihi, Morsal

AU - Seeger, Philipp

AU - Oh, Jun

AU - Grotelüschen, Rainer

AU - Busch, Philipp

AU - Mann, Oliver

AU - Hackert, Thilo

AU - Izbicki, Jakob R

AU - Yamada, Yoshito

AU - Huber, Samuel

AU - Jungraithmayr, Wolfgang

N1 - Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.

PY - 2023/12/15

Y1 - 2023/12/15

N2 - The transplantation model provides the opportunity to assess the relevance of a molecule of interest for tumor cell extravasation by using a respective genetically modified donor animal. Here, we present a protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies. We describe steps for animal preparation, lung transplantation, and tumor cell injection. We then detail procedures for the direct comparison of tumor cell spreading between the genetically modified left lung and the naive right lung parenchyma. For complete details on the use and execution of this protocol, please refer to Giannou et al. (2023).1.

AB - The transplantation model provides the opportunity to assess the relevance of a molecule of interest for tumor cell extravasation by using a respective genetically modified donor animal. Here, we present a protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies. We describe steps for animal preparation, lung transplantation, and tumor cell injection. We then detail procedures for the direct comparison of tumor cell spreading between the genetically modified left lung and the naive right lung parenchyma. For complete details on the use and execution of this protocol, please refer to Giannou et al. (2023).1.

U2 - 10.1016/j.xpro.2023.102701

DO - 10.1016/j.xpro.2023.102701

M3 - SCORING: Journal article

C2 - 37950863

VL - 4

SP - 102701

JO - STAR protocols

JF - STAR protocols

SN - 2666-1667

IS - 4

ER -