Protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies
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Protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies. / Giannou, Anastasios D; Ohm, Birte; Zazara, Dimitra E; Lücke, Jöran; Zhang, Tao; Sabihi, Morsal; Seeger, Philipp; Oh, Jun; Grotelüschen, Rainer; Busch, Philipp; Mann, Oliver; Hackert, Thilo; Izbicki, Jakob R; Yamada, Yoshito; Huber, Samuel; Jungraithmayr, Wolfgang.
in: STAR protocols, Jahrgang 4, Nr. 4, 15.12.2023, S. 102701.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies
AU - Giannou, Anastasios D
AU - Ohm, Birte
AU - Zazara, Dimitra E
AU - Lücke, Jöran
AU - Zhang, Tao
AU - Sabihi, Morsal
AU - Seeger, Philipp
AU - Oh, Jun
AU - Grotelüschen, Rainer
AU - Busch, Philipp
AU - Mann, Oliver
AU - Hackert, Thilo
AU - Izbicki, Jakob R
AU - Yamada, Yoshito
AU - Huber, Samuel
AU - Jungraithmayr, Wolfgang
N1 - Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.
PY - 2023/12/15
Y1 - 2023/12/15
N2 - The transplantation model provides the opportunity to assess the relevance of a molecule of interest for tumor cell extravasation by using a respective genetically modified donor animal. Here, we present a protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies. We describe steps for animal preparation, lung transplantation, and tumor cell injection. We then detail procedures for the direct comparison of tumor cell spreading between the genetically modified left lung and the naive right lung parenchyma. For complete details on the use and execution of this protocol, please refer to Giannou et al. (2023).1.
AB - The transplantation model provides the opportunity to assess the relevance of a molecule of interest for tumor cell extravasation by using a respective genetically modified donor animal. Here, we present a protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies. We describe steps for animal preparation, lung transplantation, and tumor cell injection. We then detail procedures for the direct comparison of tumor cell spreading between the genetically modified left lung and the naive right lung parenchyma. For complete details on the use and execution of this protocol, please refer to Giannou et al. (2023).1.
U2 - 10.1016/j.xpro.2023.102701
DO - 10.1016/j.xpro.2023.102701
M3 - SCORING: Journal article
C2 - 37950863
VL - 4
SP - 102701
JO - STAR protocols
JF - STAR protocols
SN - 2666-1667
IS - 4
ER -