Protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies
Beteiligte Einrichtungen
Abstract
The transplantation model provides the opportunity to assess the relevance of a molecule of interest for tumor cell extravasation by using a respective genetically modified donor animal. Here, we present a protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies. We describe steps for animal preparation, lung transplantation, and tumor cell injection. We then detail procedures for the direct comparison of tumor cell spreading between the genetically modified left lung and the naive right lung parenchyma. For complete details on the use and execution of this protocol, please refer to Giannou et al. (2023).1.
Bibliografische Daten
Originalsprache | Englisch |
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ISSN | 2666-1667 |
DOIs | |
Status | Veröffentlicht - 15.12.2023 |
Anmerkungen des Dekanats
Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.
PubMed | 37950863 |
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