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Plasma levels of neuron specific enolase quantify the extent of neuronal injury in murine models of ischemic stroke and multiple sclerosis

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Plasma levels of neuron specific enolase quantify the extent of neuronal injury in murine models of ischemic stroke and multiple sclerosis. / Gelderblom, Mathias; Daehn, Tristan; Schattling, Benjamin; Ludewig, Peter; Bernreuther, Christian; Arunachalam, Priyadharshini; Matschke, Jakob; Glatzel, Markus; Gerloff, Christian; Friese, Manuel A; Magnus, Tim.

in: NEUROBIOL DIS, Jahrgang 59, 01.11.2013, S. 177-82.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Gelderblom, M, Daehn, T, Schattling, B, Ludewig, P, Bernreuther, C, Arunachalam, P, Matschke, J, Glatzel, M, Gerloff, C, Friese, MA & Magnus, T 2013, 'Plasma levels of neuron specific enolase quantify the extent of neuronal injury in murine models of ischemic stroke and multiple sclerosis', NEUROBIOL DIS, Jg. 59, S. 177-82. https://doi.org/10.1016/j.nbd.2013.07.017

APA

Gelderblom, M., Daehn, T., Schattling, B., Ludewig, P., Bernreuther, C., Arunachalam, P., Matschke, J., Glatzel, M., Gerloff, C., Friese, M. A., & Magnus, T. (2013). Plasma levels of neuron specific enolase quantify the extent of neuronal injury in murine models of ischemic stroke and multiple sclerosis. NEUROBIOL DIS, 59, 177-82. https://doi.org/10.1016/j.nbd.2013.07.017

Vancouver

Gelderblom M, Daehn T, Schattling B, Ludewig P, Bernreuther C, Arunachalam P et al. Plasma levels of neuron specific enolase quantify the extent of neuronal injury in murine models of ischemic stroke and multiple sclerosis. NEUROBIOL DIS. 2013 Nov 1;59:177-82. https://doi.org/10.1016/j.nbd.2013.07.017

Bibtex

@article{687c8e57a23344948bc666e2b03f5703,
title = "Plasma levels of neuron specific enolase quantify the extent of neuronal injury in murine models of ischemic stroke and multiple sclerosis",
abstract = "OBJECTIVE: We aimed at validating a plasma biomarker for neuronal damage that can be used in acute and chronic models of neurological diseases.METHODS: We investigated two different models, middle cerebral artery occlusion followed by reperfusion and MOG35-55-induced experimental autoimmune encephalomyelitis (EAE). In stroke experiments we measured infarct sizes by magnetic resonance imaging and vital stainings and correlated them with plasma levels of neuron specific enolase (NSE) at different time points after reperfusion. Equally, in EAE experiments, we correlated NSE levels with neurological scores and histopathological damage of axons at different time points. We detected plasma NSE levels by ELISA.RESULTS: Plasma NSE levels correlated significantly with stroke size, EAE score and histopathological damage in EAE. Investigations into the dynamics of neuronal loss over time correlated well with the dynamics of NSE levels. NSE even predicted the onset of EAE, before clinical signs were recordable.CONCLUSIONS: Plasma NSE is a valid and simple experimental biomarker that allows quantifying the degree of neuronal injury in a non-invasive approach.",
keywords = "Amyloid beta-Protein Precursor, Animals, Brain, Brain Infarction, Cells, Cultured, Disease Models, Animal, Embryo, Mammalian, Glutamic Acid, Infarction, Middle Cerebral Artery, L-Lactate Dehydrogenase, Magnetic Resonance Imaging, Mice, Mice, Inbred C57BL, Microtubule-Associated Proteins, Multiple Sclerosis, Myelin-Oligodendrocyte Glycoprotein, Neurons, Peptide Fragments, Phosphopyruvate Hydratase, Time Factors",
author = "Mathias Gelderblom and Tristan Daehn and Benjamin Schattling and Peter Ludewig and Christian Bernreuther and Priyadharshini Arunachalam and Jakob Matschke and Markus Glatzel and Christian Gerloff and Friese, {Manuel A} and Tim Magnus",
note = "{\textcopyright} 2013.",
year = "2013",
month = nov,
day = "1",
doi = "10.1016/j.nbd.2013.07.017",
language = "English",
volume = "59",
pages = "177--82",
journal = "NEUROBIOL DIS",
issn = "0969-9961",
publisher = "Academic Press Inc.",

}

RIS

TY - JOUR

T1 - Plasma levels of neuron specific enolase quantify the extent of neuronal injury in murine models of ischemic stroke and multiple sclerosis

AU - Gelderblom, Mathias

AU - Daehn, Tristan

AU - Schattling, Benjamin

AU - Ludewig, Peter

AU - Bernreuther, Christian

AU - Arunachalam, Priyadharshini

AU - Matschke, Jakob

AU - Glatzel, Markus

AU - Gerloff, Christian

AU - Friese, Manuel A

AU - Magnus, Tim

N1 - © 2013.

PY - 2013/11/1

Y1 - 2013/11/1

N2 - OBJECTIVE: We aimed at validating a plasma biomarker for neuronal damage that can be used in acute and chronic models of neurological diseases.METHODS: We investigated two different models, middle cerebral artery occlusion followed by reperfusion and MOG35-55-induced experimental autoimmune encephalomyelitis (EAE). In stroke experiments we measured infarct sizes by magnetic resonance imaging and vital stainings and correlated them with plasma levels of neuron specific enolase (NSE) at different time points after reperfusion. Equally, in EAE experiments, we correlated NSE levels with neurological scores and histopathological damage of axons at different time points. We detected plasma NSE levels by ELISA.RESULTS: Plasma NSE levels correlated significantly with stroke size, EAE score and histopathological damage in EAE. Investigations into the dynamics of neuronal loss over time correlated well with the dynamics of NSE levels. NSE even predicted the onset of EAE, before clinical signs were recordable.CONCLUSIONS: Plasma NSE is a valid and simple experimental biomarker that allows quantifying the degree of neuronal injury in a non-invasive approach.

AB - OBJECTIVE: We aimed at validating a plasma biomarker for neuronal damage that can be used in acute and chronic models of neurological diseases.METHODS: We investigated two different models, middle cerebral artery occlusion followed by reperfusion and MOG35-55-induced experimental autoimmune encephalomyelitis (EAE). In stroke experiments we measured infarct sizes by magnetic resonance imaging and vital stainings and correlated them with plasma levels of neuron specific enolase (NSE) at different time points after reperfusion. Equally, in EAE experiments, we correlated NSE levels with neurological scores and histopathological damage of axons at different time points. We detected plasma NSE levels by ELISA.RESULTS: Plasma NSE levels correlated significantly with stroke size, EAE score and histopathological damage in EAE. Investigations into the dynamics of neuronal loss over time correlated well with the dynamics of NSE levels. NSE even predicted the onset of EAE, before clinical signs were recordable.CONCLUSIONS: Plasma NSE is a valid and simple experimental biomarker that allows quantifying the degree of neuronal injury in a non-invasive approach.

KW - Amyloid beta-Protein Precursor

KW - Animals

KW - Brain

KW - Brain Infarction

KW - Cells, Cultured

KW - Disease Models, Animal

KW - Embryo, Mammalian

KW - Glutamic Acid

KW - Infarction, Middle Cerebral Artery

KW - L-Lactate Dehydrogenase

KW - Magnetic Resonance Imaging

KW - Mice

KW - Mice, Inbred C57BL

KW - Microtubule-Associated Proteins

KW - Multiple Sclerosis

KW - Myelin-Oligodendrocyte Glycoprotein

KW - Neurons

KW - Peptide Fragments

KW - Phosphopyruvate Hydratase

KW - Time Factors

U2 - 10.1016/j.nbd.2013.07.017

DO - 10.1016/j.nbd.2013.07.017

M3 - SCORING: Journal article

C2 - 23932916

VL - 59

SP - 177

EP - 182

JO - NEUROBIOL DIS

JF - NEUROBIOL DIS

SN - 0969-9961

ER -