Pinpointing recurrent proviral integration sites in new models for latent HIV-1 infection
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Pinpointing recurrent proviral integration sites in new models for latent HIV-1 infection. / Lange, Ulrike C; Bialek, Julia K; Walther, Thomas; Hauber, Joachim.
in: VIRUS RES, Jahrgang 249, 02.04.2018, S. 69-75.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Pinpointing recurrent proviral integration sites in new models for latent HIV-1 infection
AU - Lange, Ulrike C
AU - Bialek, Julia K
AU - Walther, Thomas
AU - Hauber, Joachim
N1 - Copyright © 2018 Elsevier B.V. All rights reserved.
PY - 2018/4/2
Y1 - 2018/4/2
N2 - HIV infection is characterized by accumulation of proviral sequences within the human host genome. Integration of viral-derived DNA occurs at preferential loci, suggesting a site-specific crosstalk between viral sequences and human genes. We here describe a genome engineering workflow to generate models for HIV-1 infection that for the first time recapitulate proviral integration at selected genomic loci and provide unique tools to study effects of HIV proviral integration site choice. Using this workflow, we have derived two BACH2-HIV-1 reporter models that mimic largely latent integration in the clinically relevant BACH2 gene locus, which has been associated with recurrent integration and HIV-reservoir maintenance in chronically infected patients.
AB - HIV infection is characterized by accumulation of proviral sequences within the human host genome. Integration of viral-derived DNA occurs at preferential loci, suggesting a site-specific crosstalk between viral sequences and human genes. We here describe a genome engineering workflow to generate models for HIV-1 infection that for the first time recapitulate proviral integration at selected genomic loci and provide unique tools to study effects of HIV proviral integration site choice. Using this workflow, we have derived two BACH2-HIV-1 reporter models that mimic largely latent integration in the clinically relevant BACH2 gene locus, which has been associated with recurrent integration and HIV-reservoir maintenance in chronically infected patients.
KW - Journal Article
U2 - 10.1016/j.virusres.2018.03.007
DO - 10.1016/j.virusres.2018.03.007
M3 - SCORING: Journal article
C2 - 29550509
VL - 249
SP - 69
EP - 75
JO - VIRUS RES
JF - VIRUS RES
SN - 0168-1702
ER -