Neurotrophin-3 induced by tri-iodothyronine in cerebellar granule cells promotes Purkinje cell differentiation
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Neurotrophin-3 induced by tri-iodothyronine in cerebellar granule cells promotes Purkinje cell differentiation. / Lindholm, D; Castrén, E; Tsoulfas, P; Kolbeck, R; Berzaghi, M da P; Leingärtner, A; Heisenberg, C P; Tessarollo, L; Parada, L F; Thoenen, H; Tesarollo, L.
in: J CELL BIOL, Jahrgang 122, Nr. 2, 01.07.1993, S. 443-50.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Neurotrophin-3 induced by tri-iodothyronine in cerebellar granule cells promotes Purkinje cell differentiation
AU - Lindholm, D
AU - Castrén, E
AU - Tsoulfas, P
AU - Kolbeck, R
AU - Berzaghi, M da P
AU - Leingärtner, A
AU - Heisenberg, C P
AU - Tessarollo, L
AU - Parada, L F
AU - Thoenen, H
AU - Tesarollo, L
PY - 1993/7/1
Y1 - 1993/7/1
N2 - Thyroid hormones play an important role in brain development, but the mechanism(s) by which triiodothyronine (T3) mediates neuronal differentiation is poorly understood. Here we demonstrate that T3 regulates the neurotrophic factor, neurotrophin-3 (NT-3), in developing rat cerebellar granule cells both in cell culture and in vivo. In situ hybridization experiments showed that developing Purkinje cells do not express NT-3 mRNA but do express trkC, the putative neuronal receptor for NT-3. Addition of recombinant NT-3 to cerebellar cultures from embryonic rat brain induces hypertrophy and neurite sprouting of Purkinje cells, and upregulates the mRNA encoding the calcium-binding protein, calbindin-28 kD. The present study demonstrates a novel interaction between cerebellar granule neurons and developing Purkinje cells in which NT-3 induced by T3 in the granule cells promotes Purkinje cell differentiation.
AB - Thyroid hormones play an important role in brain development, but the mechanism(s) by which triiodothyronine (T3) mediates neuronal differentiation is poorly understood. Here we demonstrate that T3 regulates the neurotrophic factor, neurotrophin-3 (NT-3), in developing rat cerebellar granule cells both in cell culture and in vivo. In situ hybridization experiments showed that developing Purkinje cells do not express NT-3 mRNA but do express trkC, the putative neuronal receptor for NT-3. Addition of recombinant NT-3 to cerebellar cultures from embryonic rat brain induces hypertrophy and neurite sprouting of Purkinje cells, and upregulates the mRNA encoding the calcium-binding protein, calbindin-28 kD. The present study demonstrates a novel interaction between cerebellar granule neurons and developing Purkinje cells in which NT-3 induced by T3 in the granule cells promotes Purkinje cell differentiation.
KW - Animals
KW - Cell Differentiation
KW - Cells, Cultured
KW - Cerebellum
KW - Hypothyroidism
KW - In Situ Hybridization
KW - Membrane Glycoproteins
KW - Nerve Growth Factors
KW - Neurons
KW - Neurotrophin 3
KW - Purkinje Cells
KW - RNA, Messenger
KW - Rats
KW - Receptor, trkC
KW - Triiodothyronine
M3 - SCORING: Journal article
C2 - 8320266
VL - 122
SP - 443
EP - 450
JO - J CELL BIOL
JF - J CELL BIOL
SN - 0021-9525
IS - 2
ER -