Monitoring of hepatitis E virus in wastewater can identify clinically relevant variants
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Monitoring of hepatitis E virus in wastewater can identify clinically relevant variants. / Rau, Fiona; Elsner, Carina; Meister, Toni; Gömer, André ; Kallies, René; Dittmer, Ulf; Steinmann, Eike; Todt, Daniel.
in: LIVER INT, Jahrgang 44, Nr. 3, 03.2024, S. 637-643.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › Kurzpublikation › Forschung › Begutachtung
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TY - JOUR
T1 - Monitoring of hepatitis E virus in wastewater can identify clinically relevant variants
AU - Rau, Fiona
AU - Elsner, Carina
AU - Meister, Toni
AU - Gömer, André
AU - Kallies, René
AU - Dittmer, Ulf
AU - Steinmann, Eike
AU - Todt, Daniel
N1 - Brief Report
PY - 2024/3
Y1 - 2024/3
N2 - Hepatitis E virus (HEV) is prevalent worldwide and can cause persistent infection with severe morbidity. Antiviral treatment approaches can lead to the emergence of viral variants encoding escape mutations that may impede viral clearance. The frequency of these variants remains unknown in the human population as well as environment due to limited comprehensive data on HEV diversity. In this study, we investigated the HEV prevalence and diversity of circulating variants in environmental samples, that is, wastewater and rivers from North-Rhine Westphalia, Germany. HEV prevalence could be determined with 73% of samples tested positive for viral RNA via qRT-PCR. Using high-throughput sequencing, we were able to assess the overall genetic diversity in these samples and identified the presence of clinically relevant variants associated with drug resistance. In summary, monitoring variants from environmental samples could provide valuable insights into estimating HEV prevalence and identifying circulating variants that can impact treatment outcome.
AB - Hepatitis E virus (HEV) is prevalent worldwide and can cause persistent infection with severe morbidity. Antiviral treatment approaches can lead to the emergence of viral variants encoding escape mutations that may impede viral clearance. The frequency of these variants remains unknown in the human population as well as environment due to limited comprehensive data on HEV diversity. In this study, we investigated the HEV prevalence and diversity of circulating variants in environmental samples, that is, wastewater and rivers from North-Rhine Westphalia, Germany. HEV prevalence could be determined with 73% of samples tested positive for viral RNA via qRT-PCR. Using high-throughput sequencing, we were able to assess the overall genetic diversity in these samples and identified the presence of clinically relevant variants associated with drug resistance. In summary, monitoring variants from environmental samples could provide valuable insights into estimating HEV prevalence and identifying circulating variants that can impact treatment outcome.
U2 - 10.1111/liv.15842
DO - 10.1111/liv.15842
M3 - Short publication
C2 - 38291853
VL - 44
SP - 637
EP - 643
JO - LIVER INT
JF - LIVER INT
SN - 1478-3223
IS - 3
ER -