Molecular cytogenetic monitoring from CD34+ peripheral blood cells in myelodysplastic syndromes
Standard
Molecular cytogenetic monitoring from CD34+ peripheral blood cells in myelodysplastic syndromes : first results from a prospective multicenter German diagnostic study. / Braulke, Friederike; Jung, Klaus; Schanz, Julie; Götze, Katharina; Müller-Thomas, Catharina; Platzbecker, Uwe; Germing, Ulrich; Brümmendorf, Tim H; Bug, Gesine; Ottmann, Oliver; Giagounidis, Aristoteles A N; Stadler, Michael; Hofmann, Wolf-Karsten; Schafhausen, Philippe; Lübbert, Michael; Schlenk, Richard F; Blau, Igor W; Ganster, Christina; Pfeiffer, Sebastian; Shirneshan, Katayoon; Metz, Michael; Detken, Sven; Seraphin, Jörg; Jentsch-Ullrich, Kathleen; Böhme, Angelika; Schmidt, Burkhard; Trümper, Lorenz; Haase, Detlef.
in: Leukemia research, Jahrgang 37, Nr. 8, 01.08.2013, S. 900-6.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Molecular cytogenetic monitoring from CD34+ peripheral blood cells in myelodysplastic syndromes
T2 - first results from a prospective multicenter German diagnostic study
AU - Braulke, Friederike
AU - Jung, Klaus
AU - Schanz, Julie
AU - Götze, Katharina
AU - Müller-Thomas, Catharina
AU - Platzbecker, Uwe
AU - Germing, Ulrich
AU - Brümmendorf, Tim H
AU - Bug, Gesine
AU - Ottmann, Oliver
AU - Giagounidis, Aristoteles A N
AU - Stadler, Michael
AU - Hofmann, Wolf-Karsten
AU - Schafhausen, Philippe
AU - Lübbert, Michael
AU - Schlenk, Richard F
AU - Blau, Igor W
AU - Ganster, Christina
AU - Pfeiffer, Sebastian
AU - Shirneshan, Katayoon
AU - Metz, Michael
AU - Detken, Sven
AU - Seraphin, Jörg
AU - Jentsch-Ullrich, Kathleen
AU - Böhme, Angelika
AU - Schmidt, Burkhard
AU - Trümper, Lorenz
AU - Haase, Detlef
N1 - Copyright © 2013 Elsevier Ltd. All rights reserved.
PY - 2013/8/1
Y1 - 2013/8/1
N2 - The gold standard of cytogenetic analysis in myelodysplastic syndromes (MDS) is conventional chromosome banding (CCB) analysis of bone marrow (BM) metaphases. Most aberrations can also be detected by fluorescence-in situ-hybridization (FISH). For this prospective multicenter German diagnostic study (www.clinicaltrials.gov: #NCT01355913) 360 patients, as yet, were followed up to 3 years by sequential FISH analyses of immunomagnetically enriched CD34+ peripheral blood (PB) cells using comprehensive FISH probe panels, resulting in a total number of 19,516 FISH analyses. We demonstrate that CD34+ PB FISH correlates significantly with CCB analysis and represents a feasible method for a reliable non-invasive cytogenetic monitoring from PB.
AB - The gold standard of cytogenetic analysis in myelodysplastic syndromes (MDS) is conventional chromosome banding (CCB) analysis of bone marrow (BM) metaphases. Most aberrations can also be detected by fluorescence-in situ-hybridization (FISH). For this prospective multicenter German diagnostic study (www.clinicaltrials.gov: #NCT01355913) 360 patients, as yet, were followed up to 3 years by sequential FISH analyses of immunomagnetically enriched CD34+ peripheral blood (PB) cells using comprehensive FISH probe panels, resulting in a total number of 19,516 FISH analyses. We demonstrate that CD34+ PB FISH correlates significantly with CCB analysis and represents a feasible method for a reliable non-invasive cytogenetic monitoring from PB.
KW - Adult
KW - Aged
KW - Aged, 80 and over
KW - Antigens, CD34
KW - Antineoplastic Combined Chemotherapy Protocols
KW - Azacitidine
KW - Chromosome Aberrations
KW - Chromosome Banding
KW - Female
KW - Follow-Up Studies
KW - Germany
KW - Humans
KW - In Situ Hybridization, Fluorescence
KW - Male
KW - Middle Aged
KW - Myelodysplastic Syndromes
KW - Prospective Studies
KW - Thalidomide
KW - Time Factors
KW - Treatment Outcome
U2 - 10.1016/j.leukres.2013.03.019
DO - 10.1016/j.leukres.2013.03.019
M3 - SCORING: Journal article
C2 - 23623559
VL - 37
SP - 900
EP - 906
JO - LEUKEMIA RES
JF - LEUKEMIA RES
SN - 0145-2126
IS - 8
ER -