Microtubules Modulate F-actin Dynamics during Neuronal Polarization
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Microtubules Modulate F-actin Dynamics during Neuronal Polarization. / Zhao, Bing; Meka, Praveen; Scharrenberg, Robin; König, Theresa; Schwanke, Birgit; Kobler, Oliver; Windhorst, Sabine; Kreutz, Michael R; Mikhaylova, Marina; Calderon de Anda, Froylan.
in: SCI REP-UK, Jahrgang 7, Nr. 1, 29.08.2017, S. 9583.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Microtubules Modulate F-actin Dynamics during Neuronal Polarization
AU - Zhao, Bing
AU - Meka, Praveen
AU - Scharrenberg, Robin
AU - König, Theresa
AU - Schwanke, Birgit
AU - Kobler, Oliver
AU - Windhorst, Sabine
AU - Kreutz, Michael R
AU - Mikhaylova, Marina
AU - Calderon de Anda, Froylan
PY - 2017/8/29
Y1 - 2017/8/29
N2 - Neuronal polarization is reflected by different dynamics of microtubule and filamentous actin (F-actin). Axonal microtubules are more stable than those in the remaining neurites, while dynamics of F-actin in axonal growth cones clearly exceed those in their dendritic counterparts. However, whether a functional interplay exists between the microtubule network and F-actin dynamics in growing axons and whether this interplay is instrumental for breaking cellular symmetry is currently unknown. Here, we show that an increment on microtubule stability or number of microtubules is associated with increased F-actin dynamics. Moreover, we show that Drebrin E, an F-actin and microtubule plus-end binding protein, mediates this cross talk. Drebrin E segregates preferentially to growth cones with a higher F-actin treadmilling rate, where more microtubule plus-ends are found. Interruption of the interaction of Drebrin E with microtubules decreases F-actin dynamics and arrests neuronal polarization. Collectively the data show that microtubules modulate F-actin dynamics for initial axon extension during neuronal development.
AB - Neuronal polarization is reflected by different dynamics of microtubule and filamentous actin (F-actin). Axonal microtubules are more stable than those in the remaining neurites, while dynamics of F-actin in axonal growth cones clearly exceed those in their dendritic counterparts. However, whether a functional interplay exists between the microtubule network and F-actin dynamics in growing axons and whether this interplay is instrumental for breaking cellular symmetry is currently unknown. Here, we show that an increment on microtubule stability or number of microtubules is associated with increased F-actin dynamics. Moreover, we show that Drebrin E, an F-actin and microtubule plus-end binding protein, mediates this cross talk. Drebrin E segregates preferentially to growth cones with a higher F-actin treadmilling rate, where more microtubule plus-ends are found. Interruption of the interaction of Drebrin E with microtubules decreases F-actin dynamics and arrests neuronal polarization. Collectively the data show that microtubules modulate F-actin dynamics for initial axon extension during neuronal development.
KW - Journal Article
U2 - 10.1038/s41598-017-09832-8
DO - 10.1038/s41598-017-09832-8
M3 - SCORING: Journal article
C2 - 28851982
VL - 7
SP - 9583
JO - SCI REP-UK
JF - SCI REP-UK
SN - 2045-2322
IS - 1
ER -