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Low values of 5-hydroxymethylcytosine (5hmC), the "sixth base," are associated with anaplasia in human brain tumors

Standard

Low values of 5-hydroxymethylcytosine (5hmC), the "sixth base," are associated with anaplasia in human brain tumors. / Kraus, Theo F J; Globisch, Daniel; Wagner, Mirko; Eigenbrod, Sabina; Widmann, David; Münzel, Martin; Müller, Markus; Pfaffeneder, Toni; Hackner, Benjamin; Feiden, Wolfgang; Schüller, Ulrich; Carell, Thomas; Kretzschmar, Hans A.

in: INT J CANCER, Jahrgang 131, Nr. 7, 01.10.2012, S. 1577-90.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Kraus, TFJ, Globisch, D, Wagner, M, Eigenbrod, S, Widmann, D, Münzel, M, Müller, M, Pfaffeneder, T, Hackner, B, Feiden, W, Schüller, U, Carell, T & Kretzschmar, HA 2012, 'Low values of 5-hydroxymethylcytosine (5hmC), the "sixth base," are associated with anaplasia in human brain tumors', INT J CANCER, Jg. 131, Nr. 7, S. 1577-90. https://doi.org/10.1002/ijc.27429

APA

Kraus, T. F. J., Globisch, D., Wagner, M., Eigenbrod, S., Widmann, D., Münzel, M., Müller, M., Pfaffeneder, T., Hackner, B., Feiden, W., Schüller, U., Carell, T., & Kretzschmar, H. A. (2012). Low values of 5-hydroxymethylcytosine (5hmC), the "sixth base," are associated with anaplasia in human brain tumors. INT J CANCER, 131(7), 1577-90. https://doi.org/10.1002/ijc.27429

Vancouver

Kraus TFJ, Globisch D, Wagner M, Eigenbrod S, Widmann D, Münzel M et al. Low values of 5-hydroxymethylcytosine (5hmC), the "sixth base," are associated with anaplasia in human brain tumors. INT J CANCER. 2012 Okt 1;131(7):1577-90. https://doi.org/10.1002/ijc.27429

Bibtex

@article{789763180ecd4c4bac4239add52c8314,
title = "Low values of 5-hydroxymethylcytosine (5hmC), the {"}sixth base,{"} are associated with anaplasia in human brain tumors",
abstract = "5-Methylcytosine (5 mC) in genomic DNA has important epigenetic functions in embryonic development and tumor biology. 5-Hydroxymethylcytosine (5 hmC) is generated from 5 mC by the action of the TET (Ten-Eleven-Translocation) enzymes and may be an intermediate to further oxidation and finally demethylation of 5 mC. We have used immunohistochemistry (IHC) and isotope-based liquid chromatography mass spectrometry (LC-MS) to investigate the presence and distribution of 5 hmC in human brain and brain tumors. In the normal adult brain, IHC identified 61.5% 5 hmC positive cells in the cortex and 32.4% 5 hmC in white matter (WM) areas. In tumors, positive staining of cells ranged from 1.1% in glioblastomas (GBMs) (WHO Grade IV) to 8.9% in Grade I gliomas (pilocytic astrocytomas). In the normal adult human brain, LC-MS also showed highest values in cortical areas (1.17% 5 hmC/dG [deoxyguanosine]), in the cerebral WM we measured around 0.70% 5 hmC/dG. levels were related to tumor differentiation, ranging from lowest values of 0.078% 5 hmC/dG in GBMs (WHO Grade IV) to 0.24% 5 hmC/dG in WHO Grade II diffuse astrocytomas. 5 hmC measurements were unrelated to 5 mC values. We find that the number of 5 hmC positive cells and the amount of 5 hmC/dG in the genome that has been proposed to be related to pluripotency and lineage commitment in embryonic stem cells is also associated with brain tumor differentiation and anaplasia.",
keywords = "Adolescent, Adult, Aged, Aged, 80 and over, Anaplasia, Astrocytoma, Brain, Brain Neoplasms, Cerebral Cortex, Child, Child, Preschool, Cytosine, DNA, Epigenesis, Genetic, Female, Humans, Isocitrate Dehydrogenase, Male, Middle Aged, Mutation, Young Adult, Journal Article, Research Support, Non-U.S. Gov't",
author = "Kraus, {Theo F J} and Daniel Globisch and Mirko Wagner and Sabina Eigenbrod and David Widmann and Martin M{\"u}nzel and Markus M{\"u}ller and Toni Pfaffeneder and Benjamin Hackner and Wolfgang Feiden and Ulrich Sch{\"u}ller and Thomas Carell and Kretzschmar, {Hans A}",
note = "Copyright {\textcopyright} 2012 UICC.",
year = "2012",
month = oct,
day = "1",
doi = "10.1002/ijc.27429",
language = "English",
volume = "131",
pages = "1577--90",
journal = "INT J CANCER",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "7",

}

RIS

TY - JOUR

T1 - Low values of 5-hydroxymethylcytosine (5hmC), the "sixth base," are associated with anaplasia in human brain tumors

AU - Kraus, Theo F J

AU - Globisch, Daniel

AU - Wagner, Mirko

AU - Eigenbrod, Sabina

AU - Widmann, David

AU - Münzel, Martin

AU - Müller, Markus

AU - Pfaffeneder, Toni

AU - Hackner, Benjamin

AU - Feiden, Wolfgang

AU - Schüller, Ulrich

AU - Carell, Thomas

AU - Kretzschmar, Hans A

N1 - Copyright © 2012 UICC.

PY - 2012/10/1

Y1 - 2012/10/1

N2 - 5-Methylcytosine (5 mC) in genomic DNA has important epigenetic functions in embryonic development and tumor biology. 5-Hydroxymethylcytosine (5 hmC) is generated from 5 mC by the action of the TET (Ten-Eleven-Translocation) enzymes and may be an intermediate to further oxidation and finally demethylation of 5 mC. We have used immunohistochemistry (IHC) and isotope-based liquid chromatography mass spectrometry (LC-MS) to investigate the presence and distribution of 5 hmC in human brain and brain tumors. In the normal adult brain, IHC identified 61.5% 5 hmC positive cells in the cortex and 32.4% 5 hmC in white matter (WM) areas. In tumors, positive staining of cells ranged from 1.1% in glioblastomas (GBMs) (WHO Grade IV) to 8.9% in Grade I gliomas (pilocytic astrocytomas). In the normal adult human brain, LC-MS also showed highest values in cortical areas (1.17% 5 hmC/dG [deoxyguanosine]), in the cerebral WM we measured around 0.70% 5 hmC/dG. levels were related to tumor differentiation, ranging from lowest values of 0.078% 5 hmC/dG in GBMs (WHO Grade IV) to 0.24% 5 hmC/dG in WHO Grade II diffuse astrocytomas. 5 hmC measurements were unrelated to 5 mC values. We find that the number of 5 hmC positive cells and the amount of 5 hmC/dG in the genome that has been proposed to be related to pluripotency and lineage commitment in embryonic stem cells is also associated with brain tumor differentiation and anaplasia.

AB - 5-Methylcytosine (5 mC) in genomic DNA has important epigenetic functions in embryonic development and tumor biology. 5-Hydroxymethylcytosine (5 hmC) is generated from 5 mC by the action of the TET (Ten-Eleven-Translocation) enzymes and may be an intermediate to further oxidation and finally demethylation of 5 mC. We have used immunohistochemistry (IHC) and isotope-based liquid chromatography mass spectrometry (LC-MS) to investigate the presence and distribution of 5 hmC in human brain and brain tumors. In the normal adult brain, IHC identified 61.5% 5 hmC positive cells in the cortex and 32.4% 5 hmC in white matter (WM) areas. In tumors, positive staining of cells ranged from 1.1% in glioblastomas (GBMs) (WHO Grade IV) to 8.9% in Grade I gliomas (pilocytic astrocytomas). In the normal adult human brain, LC-MS also showed highest values in cortical areas (1.17% 5 hmC/dG [deoxyguanosine]), in the cerebral WM we measured around 0.70% 5 hmC/dG. levels were related to tumor differentiation, ranging from lowest values of 0.078% 5 hmC/dG in GBMs (WHO Grade IV) to 0.24% 5 hmC/dG in WHO Grade II diffuse astrocytomas. 5 hmC measurements were unrelated to 5 mC values. We find that the number of 5 hmC positive cells and the amount of 5 hmC/dG in the genome that has been proposed to be related to pluripotency and lineage commitment in embryonic stem cells is also associated with brain tumor differentiation and anaplasia.

KW - Adolescent

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Anaplasia

KW - Astrocytoma

KW - Brain

KW - Brain Neoplasms

KW - Cerebral Cortex

KW - Child

KW - Child, Preschool

KW - Cytosine

KW - DNA

KW - Epigenesis, Genetic

KW - Female

KW - Humans

KW - Isocitrate Dehydrogenase

KW - Male

KW - Middle Aged

KW - Mutation

KW - Young Adult

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1002/ijc.27429

DO - 10.1002/ijc.27429

M3 - SCORING: Journal article

C2 - 22234893

VL - 131

SP - 1577

EP - 1590

JO - INT J CANCER

JF - INT J CANCER

SN - 0020-7136

IS - 7

ER -