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Limbal BCAM expression identifies a proliferative progenitor population capable of holoclone formation and corneal differentiation

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Limbal BCAM expression identifies a proliferative progenitor population capable of holoclone formation and corneal differentiation. / Sasamoto, Yuzuru; Lee, Catherine A A; Wilson, Brian J; Buerger, Florian; Martin, Gabrielle; Mishra, Ananda; Kiritoshi, Shoko; Tran, Johnathan; Gonzalez, Gabriel; Hildebrandt, Friedhelm; Jo, Vickie Y; Lian, Christine G; Murphy, George F; Ksander, Bruce R; Frank, Markus H; Frank, Natasha Y.

in: CELL REP, Jahrgang 40, Nr. 6, 09.08.2022, S. 111166.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Sasamoto, Y, Lee, CAA, Wilson, BJ, Buerger, F, Martin, G, Mishra, A, Kiritoshi, S, Tran, J, Gonzalez, G, Hildebrandt, F, Jo, VY, Lian, CG, Murphy, GF, Ksander, BR, Frank, MH & Frank, NY 2022, 'Limbal BCAM expression identifies a proliferative progenitor population capable of holoclone formation and corneal differentiation', CELL REP, Jg. 40, Nr. 6, S. 111166. https://doi.org/10.1016/j.celrep.2022.111166

APA

Sasamoto, Y., Lee, C. A. A., Wilson, B. J., Buerger, F., Martin, G., Mishra, A., Kiritoshi, S., Tran, J., Gonzalez, G., Hildebrandt, F., Jo, V. Y., Lian, C. G., Murphy, G. F., Ksander, B. R., Frank, M. H., & Frank, N. Y. (2022). Limbal BCAM expression identifies a proliferative progenitor population capable of holoclone formation and corneal differentiation. CELL REP, 40(6), 111166. https://doi.org/10.1016/j.celrep.2022.111166

Vancouver

Sasamoto Y, Lee CAA, Wilson BJ, Buerger F, Martin G, Mishra A et al. Limbal BCAM expression identifies a proliferative progenitor population capable of holoclone formation and corneal differentiation. CELL REP. 2022 Aug 9;40(6):111166. https://doi.org/10.1016/j.celrep.2022.111166

Bibtex

@article{374fa1ebcfdd4bf9a5602cbc21bf05f0,
title = "Limbal BCAM expression identifies a proliferative progenitor population capable of holoclone formation and corneal differentiation",
abstract = "The corneal epithelium is renowned for high regenerative potential, which is dependent on the coordinated function of its diverse progenitor subpopulations. However, the molecular pathways governing corneal epithelial progenitor differentiation are incompletely understood. Here, we identify a highly proliferative limbal epithelial progenitor subpopulation characterized by expression of basal cell adhesion molecule (BCAM) that is capable of holocone formation and corneal epithelial sheet generation. BCAM-positive cells can be found among ABCB5-positive limbal stem cells (LSCs) as well as among ABCB5-negative limbal epithelial cell populations. Mechanistically, we show that BCAM is functionally required for cellular migration and differentiation and that its expression is regulated by the transcription factor p63. In aggregate, our study identifies limbal BCAM expression as a marker of highly proliferative corneal epithelial progenitor cells and defines the role of BCAM as a critical molecular mediator of corneal epithelial differentiation.",
keywords = "Cell Differentiation, Cells, Cultured, Cornea, Epithelial Cells/metabolism, Epithelium, Corneal, Limbus Corneae/metabolism, Stem Cells/metabolism",
author = "Yuzuru Sasamoto and Lee, {Catherine A A} and Wilson, {Brian J} and Florian Buerger and Gabrielle Martin and Ananda Mishra and Shoko Kiritoshi and Johnathan Tran and Gabriel Gonzalez and Friedhelm Hildebrandt and Jo, {Vickie Y} and Lian, {Christine G} and Murphy, {George F} and Ksander, {Bruce R} and Frank, {Markus H} and Frank, {Natasha Y}",
note = "Published by Elsevier Inc.",
year = "2022",
month = aug,
day = "9",
doi = "10.1016/j.celrep.2022.111166",
language = "English",
volume = "40",
pages = "111166",
journal = "CELL REP",
issn = "2211-1247",
publisher = "Elsevier",
number = "6",

}

RIS

TY - JOUR

T1 - Limbal BCAM expression identifies a proliferative progenitor population capable of holoclone formation and corneal differentiation

AU - Sasamoto, Yuzuru

AU - Lee, Catherine A A

AU - Wilson, Brian J

AU - Buerger, Florian

AU - Martin, Gabrielle

AU - Mishra, Ananda

AU - Kiritoshi, Shoko

AU - Tran, Johnathan

AU - Gonzalez, Gabriel

AU - Hildebrandt, Friedhelm

AU - Jo, Vickie Y

AU - Lian, Christine G

AU - Murphy, George F

AU - Ksander, Bruce R

AU - Frank, Markus H

AU - Frank, Natasha Y

N1 - Published by Elsevier Inc.

PY - 2022/8/9

Y1 - 2022/8/9

N2 - The corneal epithelium is renowned for high regenerative potential, which is dependent on the coordinated function of its diverse progenitor subpopulations. However, the molecular pathways governing corneal epithelial progenitor differentiation are incompletely understood. Here, we identify a highly proliferative limbal epithelial progenitor subpopulation characterized by expression of basal cell adhesion molecule (BCAM) that is capable of holocone formation and corneal epithelial sheet generation. BCAM-positive cells can be found among ABCB5-positive limbal stem cells (LSCs) as well as among ABCB5-negative limbal epithelial cell populations. Mechanistically, we show that BCAM is functionally required for cellular migration and differentiation and that its expression is regulated by the transcription factor p63. In aggregate, our study identifies limbal BCAM expression as a marker of highly proliferative corneal epithelial progenitor cells and defines the role of BCAM as a critical molecular mediator of corneal epithelial differentiation.

AB - The corneal epithelium is renowned for high regenerative potential, which is dependent on the coordinated function of its diverse progenitor subpopulations. However, the molecular pathways governing corneal epithelial progenitor differentiation are incompletely understood. Here, we identify a highly proliferative limbal epithelial progenitor subpopulation characterized by expression of basal cell adhesion molecule (BCAM) that is capable of holocone formation and corneal epithelial sheet generation. BCAM-positive cells can be found among ABCB5-positive limbal stem cells (LSCs) as well as among ABCB5-negative limbal epithelial cell populations. Mechanistically, we show that BCAM is functionally required for cellular migration and differentiation and that its expression is regulated by the transcription factor p63. In aggregate, our study identifies limbal BCAM expression as a marker of highly proliferative corneal epithelial progenitor cells and defines the role of BCAM as a critical molecular mediator of corneal epithelial differentiation.

KW - Cell Differentiation

KW - Cells, Cultured

KW - Cornea

KW - Epithelial Cells/metabolism

KW - Epithelium, Corneal

KW - Limbus Corneae/metabolism

KW - Stem Cells/metabolism

U2 - 10.1016/j.celrep.2022.111166

DO - 10.1016/j.celrep.2022.111166

M3 - SCORING: Journal article

C2 - 35947947

VL - 40

SP - 111166

JO - CELL REP

JF - CELL REP

SN - 2211-1247

IS - 6

ER -