Interaction of the TGGCA-binding protein with upstream sequences is required for efficient transcription of mouse mammary tumor virus
Standard
Interaction of the TGGCA-binding protein with upstream sequences is required for efficient transcription of mouse mammary tumor virus. / Miksicek, R; Borgmeyer, U; Nowock, J.
in: EMBO J, Jahrgang 6, Nr. 5, 05.1987, S. 1355-60.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Interaction of the TGGCA-binding protein with upstream sequences is required for efficient transcription of mouse mammary tumor virus
AU - Miksicek, R
AU - Borgmeyer, U
AU - Nowock, J
PY - 1987/5
Y1 - 1987/5
N2 - A high-affinity binding site for the TGGCA-binding protein, also known as nuclear factor I, has previously been shown to reside within the mouse mammary tumor virus (MMTV) long terminal repeat. We have introduced mutations into this binding site to test the importance of this ubiquitous nuclear protein in MMTV transcription. Mutations which abolish the binding of the TGGCA protein in vitro are shown to impair strongly glucocorticoid-induced transcription from this promoter in vivo. These data demonstrate that the TGGCA-binding protein is a multifunctional DNA-binding protein, capable of serving a transcriptional role in the case of MMTV, in addition to its known involvement in the replication of adenovirus.
AB - A high-affinity binding site for the TGGCA-binding protein, also known as nuclear factor I, has previously been shown to reside within the mouse mammary tumor virus (MMTV) long terminal repeat. We have introduced mutations into this binding site to test the importance of this ubiquitous nuclear protein in MMTV transcription. Mutations which abolish the binding of the TGGCA protein in vitro are shown to impair strongly glucocorticoid-induced transcription from this promoter in vivo. These data demonstrate that the TGGCA-binding protein is a multifunctional DNA-binding protein, capable of serving a transcriptional role in the case of MMTV, in addition to its known involvement in the replication of adenovirus.
KW - Animals
KW - Base Sequence
KW - CCAAT-Enhancer-Binding Proteins
KW - Cell Line
KW - Cell Nucleus
KW - DNA-Binding Proteins
KW - Genes, Viral
KW - Humans
KW - Liver
KW - Mammary Tumor Virus, Mouse
KW - Mice
KW - NFI Transcription Factors
KW - Nuclear Proteins
KW - Protein Binding
KW - Proto-Oncogenes
KW - Transcription Factors
KW - Transcription, Genetic
KW - Y-Box-Binding Protein 1
M3 - SCORING: Journal article
C2 - 3038519
VL - 6
SP - 1355
EP - 1360
JO - EMBO J
JF - EMBO J
SN - 0261-4189
IS - 5
ER -