Integrin β1 controls VE-cadherin localization and blood vessel stability
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Integrin β1 controls VE-cadherin localization and blood vessel stability. / Yamamoto, Hiroyuki; Ehling, Manuel; Kato, Katsuhiro; Kanai, Kenichi; van Lessen, Max; Frye, Maike; Zeuschner, Dagmar; Nakayama, Masanori; Vestweber, Dietmar; Adams, Ralf H.
in: NAT COMMUN, Jahrgang 6, 10.03.2015, S. 6429.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Integrin β1 controls VE-cadherin localization and blood vessel stability
AU - Yamamoto, Hiroyuki
AU - Ehling, Manuel
AU - Kato, Katsuhiro
AU - Kanai, Kenichi
AU - van Lessen, Max
AU - Frye, Maike
AU - Zeuschner, Dagmar
AU - Nakayama, Masanori
AU - Vestweber, Dietmar
AU - Adams, Ralf H
PY - 2015/3/10
Y1 - 2015/3/10
N2 - Angiogenic blood vessel growth requires several distinct but integrated cellular activities. Endothelial cell sprouting and proliferation lead to the expansion of the vasculature and give rise to a highly branched, immature plexus, which is subsequently reorganized into a mature and stable network. Although it is known that integrin-mediated cell-matrix interactions are indispensable for embryonic angiogenesis, little is known about the function of integrins in different steps of vascular morphogenesis. Here, by investigating the integrin β1-subunit with inducible and endothelial-specific gene targeting in the postnatal mouse retina, we show that β1 integrin promotes endothelial sprouting but is a negative regulator of proliferation. In maturing vessels, integrin β1 is indispensable for proper localization of VE-cadherin and thereby cell-cell junction integrity. The sum of our findings establishes that integrin β1 has critical functions in the growing and maturing vasculature, and is required for the formation of stable, non-leaky blood vessels.
AB - Angiogenic blood vessel growth requires several distinct but integrated cellular activities. Endothelial cell sprouting and proliferation lead to the expansion of the vasculature and give rise to a highly branched, immature plexus, which is subsequently reorganized into a mature and stable network. Although it is known that integrin-mediated cell-matrix interactions are indispensable for embryonic angiogenesis, little is known about the function of integrins in different steps of vascular morphogenesis. Here, by investigating the integrin β1-subunit with inducible and endothelial-specific gene targeting in the postnatal mouse retina, we show that β1 integrin promotes endothelial sprouting but is a negative regulator of proliferation. In maturing vessels, integrin β1 is indispensable for proper localization of VE-cadherin and thereby cell-cell junction integrity. The sum of our findings establishes that integrin β1 has critical functions in the growing and maturing vasculature, and is required for the formation of stable, non-leaky blood vessels.
KW - Animals
KW - Antigens, CD
KW - Blotting, Western
KW - Brain
KW - Cadherins
KW - Cell Proliferation
KW - Endothelium
KW - Gene Targeting
KW - Image Processing, Computer-Assisted
KW - Immunohistochemistry
KW - Immunoprecipitation
KW - Integrin beta1
KW - Intercellular Junctions
KW - Mice
KW - Microscopy, Electron
KW - Morphogenesis
KW - Neovascularization, Physiologic
KW - RNA, Small Interfering
KW - Real-Time Polymerase Chain Reaction
KW - Retinal Vessels
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1038/ncomms7429
DO - 10.1038/ncomms7429
M3 - SCORING: Journal article
C2 - 25752958
VL - 6
SP - 6429
JO - NAT COMMUN
JF - NAT COMMUN
SN - 2041-1723
ER -