Induction of IL-22-Producing CD4+ T Cells by Segmented Filamentous Bacteria Independent of Classical Th17 Cells
Beteiligte Einrichtungen
Abstract
The intestinal microbiota modulates IL-22 production in the intestine, including the induction of IL-22-producing CD4+ T helper cells. Which specific bacteria are responsible for the induction of these cells is less well understood. Here, we demonstrate through the use of novel gnotobiotic knock-in reporter mice that segmented filamentous bacteria (SFB), which are known for their ability to induce Th17 cells, also induce distinct IL-17A negative CD4+ T cell populations in the intestine. A subset of these cells instead produces IL-22 upon restimulation ex vivo and also during enteric infections. Furthermore, they produce a distinct set of cytokines compared to Th17 cells including the differential expression of IL-17F and IFN-γ. Importantly, genetic models demonstrate that these cells, presumably Th22 cells, develop independently of intestinal Th17 cells. Together, our data identifies that besides Th17, SFB also induces CD4+ T cell populations, which serve as immediate source of IL-22 during intestinal inflammation.
Bibliografische Daten
Originalsprache | Englisch |
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Aufsatznummer | 671331 |
ISSN | 1664-3224 |
DOIs | |
Status | Veröffentlicht - 08.09.2021 |
Anmerkungen des Dekanats
Funding Information:
The project was supported by the Helmholtz Association (VH-NG-933 to TS), by the DFG (STR-1343/1 to TS), the EU (StG337251 to SH) and the ERC (CoG 865466 to SH).
Publisher Copyright:
© Copyright © 2021 Roy, de Oliveira, Galvez, Gronow, Basic, Perez, Gagliani, Bleich, Huber and Strowig.