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Implantation of engineered tissue in the rat heart

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Implantation of engineered tissue in the rat heart. / Sill, Bjoern; Alpatov, Ivan V; Pacak, Christina A; Cowan, Douglas B.

in: JOVE-J VIS EXP, Nr. 28, 24.06.2009.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Sill, B, Alpatov, IV, Pacak, CA & Cowan, DB 2009, 'Implantation of engineered tissue in the rat heart', JOVE-J VIS EXP, Nr. 28. https://doi.org/10.3791/1139

APA

Sill, B., Alpatov, I. V., Pacak, C. A., & Cowan, D. B. (2009). Implantation of engineered tissue in the rat heart. JOVE-J VIS EXP, (28). https://doi.org/10.3791/1139

Vancouver

Sill B, Alpatov IV, Pacak CA, Cowan DB. Implantation of engineered tissue in the rat heart. JOVE-J VIS EXP. 2009 Jun 24;(28). https://doi.org/10.3791/1139

Bibtex

@article{ca7682e392dc4e37aaeb7adaebcad9d5,
title = "Implantation of engineered tissue in the rat heart",
abstract = "Rodent surgery is often an important component in assessing the utility of engineered tissues. A wide variety of surgical procedures can be performed in common laboratory rats or mice and these quite frequently serve as an intermediate step between bench-top experiments and large animal testing or human trials. Given that rodents provide an established, cost-effective, and physiologically-relevant model system in which to test novel combinations of scaffolding materials and cells, they are particularly well-suited for cardiovascular tissue engineering studies. Presently, we describe an open-heart surgical procedure to implant engineered tissue containing myogenic progenitor cells in the atrioventricular (AV) groove of a rat heart. These implants are intended to create an electrical conduit between the right atrium and right ventricle with the ultimate goal of providing an alternative treatment to conventional pacemaker implantation in pediatric patients with complete heart block. The engineered tissue is implanted in the AV-groove by means of a thoracotomy. For our purposes, Lewis rats are anesthetized and invasively ventilated to maintain positive airway pressure during the sterile surgical procedure. The approach to the heart is performed by a right thoracotomy through an antero-lateral incision at the 5(th) intercostal space. The tissue construct is fixed in the AV groove using a single 7-0 Prolene suture and positioned between the right ventricle and atrium at the ventral portion of the heart. The epicardium is partially removed to allow direct contact between the recipient myocardial cells and those contained in the engineered tissue. Following implantation, the chest wall is closed in layers, any pneumothorax is evacuated, and the animal is extubated and treated with analgesic.",
keywords = "Animals, Cardiac Surgical Procedures/methods, Heart Block/therapy, Rats, Rats, Inbred Lew, Stem Cells/cytology, Thoracotomy/methods, Tissue Engineering/methods, Tissue Transplantation/methods",
author = "Bjoern Sill and Alpatov, {Ivan V} and Pacak, {Christina A} and Cowan, {Douglas B}",
year = "2009",
month = jun,
day = "24",
doi = "10.3791/1139",
language = "English",
journal = "JOVE-J VIS EXP",
issn = "1940-087X",
publisher = "MYJoVE Corporation",
number = "28",

}

RIS

TY - JOUR

T1 - Implantation of engineered tissue in the rat heart

AU - Sill, Bjoern

AU - Alpatov, Ivan V

AU - Pacak, Christina A

AU - Cowan, Douglas B

PY - 2009/6/24

Y1 - 2009/6/24

N2 - Rodent surgery is often an important component in assessing the utility of engineered tissues. A wide variety of surgical procedures can be performed in common laboratory rats or mice and these quite frequently serve as an intermediate step between bench-top experiments and large animal testing or human trials. Given that rodents provide an established, cost-effective, and physiologically-relevant model system in which to test novel combinations of scaffolding materials and cells, they are particularly well-suited for cardiovascular tissue engineering studies. Presently, we describe an open-heart surgical procedure to implant engineered tissue containing myogenic progenitor cells in the atrioventricular (AV) groove of a rat heart. These implants are intended to create an electrical conduit between the right atrium and right ventricle with the ultimate goal of providing an alternative treatment to conventional pacemaker implantation in pediatric patients with complete heart block. The engineered tissue is implanted in the AV-groove by means of a thoracotomy. For our purposes, Lewis rats are anesthetized and invasively ventilated to maintain positive airway pressure during the sterile surgical procedure. The approach to the heart is performed by a right thoracotomy through an antero-lateral incision at the 5(th) intercostal space. The tissue construct is fixed in the AV groove using a single 7-0 Prolene suture and positioned between the right ventricle and atrium at the ventral portion of the heart. The epicardium is partially removed to allow direct contact between the recipient myocardial cells and those contained in the engineered tissue. Following implantation, the chest wall is closed in layers, any pneumothorax is evacuated, and the animal is extubated and treated with analgesic.

AB - Rodent surgery is often an important component in assessing the utility of engineered tissues. A wide variety of surgical procedures can be performed in common laboratory rats or mice and these quite frequently serve as an intermediate step between bench-top experiments and large animal testing or human trials. Given that rodents provide an established, cost-effective, and physiologically-relevant model system in which to test novel combinations of scaffolding materials and cells, they are particularly well-suited for cardiovascular tissue engineering studies. Presently, we describe an open-heart surgical procedure to implant engineered tissue containing myogenic progenitor cells in the atrioventricular (AV) groove of a rat heart. These implants are intended to create an electrical conduit between the right atrium and right ventricle with the ultimate goal of providing an alternative treatment to conventional pacemaker implantation in pediatric patients with complete heart block. The engineered tissue is implanted in the AV-groove by means of a thoracotomy. For our purposes, Lewis rats are anesthetized and invasively ventilated to maintain positive airway pressure during the sterile surgical procedure. The approach to the heart is performed by a right thoracotomy through an antero-lateral incision at the 5(th) intercostal space. The tissue construct is fixed in the AV groove using a single 7-0 Prolene suture and positioned between the right ventricle and atrium at the ventral portion of the heart. The epicardium is partially removed to allow direct contact between the recipient myocardial cells and those contained in the engineered tissue. Following implantation, the chest wall is closed in layers, any pneumothorax is evacuated, and the animal is extubated and treated with analgesic.

KW - Animals

KW - Cardiac Surgical Procedures/methods

KW - Heart Block/therapy

KW - Rats

KW - Rats, Inbred Lew

KW - Stem Cells/cytology

KW - Thoracotomy/methods

KW - Tissue Engineering/methods

KW - Tissue Transplantation/methods

U2 - 10.3791/1139

DO - 10.3791/1139

M3 - SCORING: Journal article

C2 - 19553905

JO - JOVE-J VIS EXP

JF - JOVE-J VIS EXP

SN - 1940-087X

IS - 28

ER -