Implantation of alloplastic material increases survival of mice subsequently exposed to polymicrobial sepsis
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Implantation of alloplastic material increases survival of mice subsequently exposed to polymicrobial sepsis. / Kessler, Wolfram; Ebker, Tobias; Koerner, Pia; Poetschke, Christian; Cziupka, Katharina; Traeger, Tobias; Westerholt, Alexandra; Mehmcke, Hendrik; Neher, Friederike; Jack, Robert S; Heidecke, Claus-Dieter; Maier, Stefan.
in: LANGENBECK ARCH SURG, Jahrgang 395, Nr. 2, 02.2010, S. 157-62.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Implantation of alloplastic material increases survival of mice subsequently exposed to polymicrobial sepsis
AU - Kessler, Wolfram
AU - Ebker, Tobias
AU - Koerner, Pia
AU - Poetschke, Christian
AU - Cziupka, Katharina
AU - Traeger, Tobias
AU - Westerholt, Alexandra
AU - Mehmcke, Hendrik
AU - Neher, Friederike
AU - Jack, Robert S
AU - Heidecke, Claus-Dieter
AU - Maier, Stefan
PY - 2010/2
Y1 - 2010/2
N2 - PURPOSE: Major surgery can modulate the immune system and by this the clinical course of following complications. Effects of minor surgical treatments on the immune system and septic complications are poorly understood.MATERIALS AND METHODS: We investigated the effect of a minor surgical procedure--the implantation of an osmotic pump--on the outcome of experimental polymicrobial sepsis (colon ascendens stent-induced peritonitis, CASP) in mice.RESULTS: Animals with pumps implanted 3 days prior to CASP showed an attenuated clinical course of sepsis and increased survival. While measured serum cytokine levels were not affected by the minor surgical stress of pump implantation, splenocyte secretion of IFN-gamma in response to lipopolysaccharide was increased.CONCLUSION: The early implantation of alloplastic material modulates the immune system and leads to an increased survival of a polymicrobial sepsis. Identifying the molecular nature of this effect might point the way to a new therapeutic approach to reduce sepsis mortality.
AB - PURPOSE: Major surgery can modulate the immune system and by this the clinical course of following complications. Effects of minor surgical treatments on the immune system and septic complications are poorly understood.MATERIALS AND METHODS: We investigated the effect of a minor surgical procedure--the implantation of an osmotic pump--on the outcome of experimental polymicrobial sepsis (colon ascendens stent-induced peritonitis, CASP) in mice.RESULTS: Animals with pumps implanted 3 days prior to CASP showed an attenuated clinical course of sepsis and increased survival. While measured serum cytokine levels were not affected by the minor surgical stress of pump implantation, splenocyte secretion of IFN-gamma in response to lipopolysaccharide was increased.CONCLUSION: The early implantation of alloplastic material modulates the immune system and leads to an increased survival of a polymicrobial sepsis. Identifying the molecular nature of this effect might point the way to a new therapeutic approach to reduce sepsis mortality.
KW - Animals
KW - Chemokines
KW - Colon, Ascending
KW - Cytokines
KW - Disease Models, Animal
KW - Down-Regulation
KW - Female
KW - Immunocompetence
KW - Inflammation
KW - Lipopolysaccharides
KW - Mice
KW - Mice, Inbred C57BL
KW - Peritonitis
KW - Prosthesis Implantation
KW - Sepsis
KW - Severity of Illness Index
KW - Single-Blind Method
KW - Spleen
KW - Statistics, Nonparametric
KW - Stents
KW - Survival Rate
KW - Time Factors
KW - Vagotomy
U2 - 10.1007/s00423-009-0463-2
DO - 10.1007/s00423-009-0463-2
M3 - SCORING: Journal article
C2 - 19172290
VL - 395
SP - 157
EP - 162
JO - LANGENBECK ARCH SURG
JF - LANGENBECK ARCH SURG
SN - 1435-2443
IS - 2
ER -