IL-17 production by CSF lymphocytes as a biomarker for cerebral vasculitis
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IL-17 production by CSF lymphocytes as a biomarker for cerebral vasculitis. / Thom, Vivien; Schmidt, Sabrina; Gelderblom, Mathias; Hackbusch, Romy ; Kolster, Manuela; Schuster, Simon; Thomalla, Götz; Keminer, Oliver; Pleß, Ole; Bernreuther, Christian; Glatzel, Markus; Wegscheider, Karl; Gerloff, Christian; Magnus, Tim; Tolosa, Eva.
in: NEUROL-NEUROIMMUNOL, Jahrgang 3, Nr. 2, 21.03.2016, S. e214.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - IL-17 production by CSF lymphocytes as a biomarker for cerebral vasculitis
AU - Thom, Vivien
AU - Schmidt, Sabrina
AU - Gelderblom, Mathias
AU - Hackbusch, Romy
AU - Kolster, Manuela
AU - Schuster, Simon
AU - Thomalla, Götz
AU - Keminer, Oliver
AU - Pleß, Ole
AU - Bernreuther, Christian
AU - Glatzel, Markus
AU - Wegscheider, Karl
AU - Gerloff, Christian
AU - Magnus, Tim
AU - Tolosa, Eva
PY - 2016/3/21
Y1 - 2016/3/21
N2 - OBJECTIVE: To explore the possibility of using interleukin-17 (IL-17) production by CD4+ T cells in the CSF as a potential biomarker for cerebral vasculitis in stroke patients.METHODS: In this consecutive case study, we performed prospective analysis of CSF and blood in patients admitted to a university medical center with symptoms of stroke and suspected cerebral vasculitis. Flow cytometry was performed for intracellular detection of inflammatory cytokines in peripheral blood lymphocytes and expanded T cells from CSF.RESULTS: CSF CD4+ lymphocytes from patients with cerebral vasculitis showed significantly higher levels of the proinflammatory cytokine IL-17 compared to patients with stroke not due to vasculitis or with other, noninflammatory neurologic diseases. There was no difference in the production of interferon-γ in the CSF and no overall differences in the relative frequencies of peripheral immune cells.CONCLUSIONS: Intracellular IL-17 in CSF cells is potentially useful in discriminating cerebral vasculitis as a rare cause in patients presenting with ischemic stroke.CLASSIFICATION OF EVIDENCE: This study provides Class II evidence that an increased proportion of IL-17-producing CD4+ cells in CSF of patients presenting with stroke symptoms is indicative of cerebral vasculitis (sensitivity 73%, 95% confidence interval [CI] 39-94%; specificity 100%, 95% CI 74%-100%).
AB - OBJECTIVE: To explore the possibility of using interleukin-17 (IL-17) production by CD4+ T cells in the CSF as a potential biomarker for cerebral vasculitis in stroke patients.METHODS: In this consecutive case study, we performed prospective analysis of CSF and blood in patients admitted to a university medical center with symptoms of stroke and suspected cerebral vasculitis. Flow cytometry was performed for intracellular detection of inflammatory cytokines in peripheral blood lymphocytes and expanded T cells from CSF.RESULTS: CSF CD4+ lymphocytes from patients with cerebral vasculitis showed significantly higher levels of the proinflammatory cytokine IL-17 compared to patients with stroke not due to vasculitis or with other, noninflammatory neurologic diseases. There was no difference in the production of interferon-γ in the CSF and no overall differences in the relative frequencies of peripheral immune cells.CONCLUSIONS: Intracellular IL-17 in CSF cells is potentially useful in discriminating cerebral vasculitis as a rare cause in patients presenting with ischemic stroke.CLASSIFICATION OF EVIDENCE: This study provides Class II evidence that an increased proportion of IL-17-producing CD4+ cells in CSF of patients presenting with stroke symptoms is indicative of cerebral vasculitis (sensitivity 73%, 95% confidence interval [CI] 39-94%; specificity 100%, 95% CI 74%-100%).
U2 - 10.1212/NXI.0000000000000214
DO - 10.1212/NXI.0000000000000214
M3 - SCORING: Journal article
C2 - 27144213
VL - 3
SP - e214
JO - NEUROL-NEUROIMMUNOL
JF - NEUROL-NEUROIMMUNOL
SN - 2332-7812
IS - 2
ER -
