Human pluripotent stem cell-derived cardiomyocytes for studying energy metabolism
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Human pluripotent stem cell-derived cardiomyocytes for studying energy metabolism. / Ulmer, Bärbel M; Eschenhagen, Thomas.
in: BBA-MOL CELL RES, Jahrgang 1867, Nr. 3, 03.2020, S. 118471.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Review › Forschung
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TY - JOUR
T1 - Human pluripotent stem cell-derived cardiomyocytes for studying energy metabolism
AU - Ulmer, Bärbel M
AU - Eschenhagen, Thomas
N1 - Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.
PY - 2020/3
Y1 - 2020/3
N2 - Cardiomyocyte energy metabolism is altered in heart failure, and primary defects of metabolic pathways can cause heart failure. Studying cardiac energetics in rodent models has principal shortcomings, raising the question to which extent human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CM) can provide an alternative. As metabolic maturation of CM occurs mostly after birth during developmental hypertrophy, the immaturity of hiPSC-CM is an important limitation. Here we shortly review the physiological drivers of metabolic maturation and concentrate on methods to mature hiPSC-CM with the goal to benchmark the metabolic state of hiPSC-CM against in vivo data and to see how far known abnormalities in inherited metabolic disorders can be modeled in hiPSC-CM. The current data indicate that hiPSC-CM, despite their immature, approximately mid-fetal state of energy metabolism, faithfully recapitulate some basic metabolic disease mechanisms. Efforts to improve their metabolic maturity are underway and shall improve the validity of this model.
AB - Cardiomyocyte energy metabolism is altered in heart failure, and primary defects of metabolic pathways can cause heart failure. Studying cardiac energetics in rodent models has principal shortcomings, raising the question to which extent human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CM) can provide an alternative. As metabolic maturation of CM occurs mostly after birth during developmental hypertrophy, the immaturity of hiPSC-CM is an important limitation. Here we shortly review the physiological drivers of metabolic maturation and concentrate on methods to mature hiPSC-CM with the goal to benchmark the metabolic state of hiPSC-CM against in vivo data and to see how far known abnormalities in inherited metabolic disorders can be modeled in hiPSC-CM. The current data indicate that hiPSC-CM, despite their immature, approximately mid-fetal state of energy metabolism, faithfully recapitulate some basic metabolic disease mechanisms. Efforts to improve their metabolic maturity are underway and shall improve the validity of this model.
KW - Cell Differentiation/genetics
KW - Energy Metabolism/genetics
KW - Humans
KW - Induced Pluripotent Stem Cells/metabolism
KW - Myocytes, Cardiac/metabolism
U2 - 10.1016/j.bbamcr.2019.04.001
DO - 10.1016/j.bbamcr.2019.04.001
M3 - SCORING: Review article
C2 - 30954570
VL - 1867
SP - 118471
JO - BBA-MOL CELL RES
JF - BBA-MOL CELL RES
SN - 0167-4889
IS - 3
ER -